We narrowed to 2,617 results for: pCas

Showing: 381 - 400 of 2617 results

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pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

Plasmid

#110302

Purpose

Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

Depositor

Chris Kok-Lung Chan

Publication

Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p53-binding protein 1 (TP53BP1 Human)

Expression

Mammalian

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-TUBB

Plasmid

#66942

Purpose

CRISPaint target selector TUBB

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA TUBB

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-ACTG1

Plasmid

#66943

Purpose

CRISPaint target selector ACTG1

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA ACTG1

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCast

Plasmid

#200169

Purpose

E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

Depositor

Yong-Su Jin

Publication

Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

June 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCas9CR5-NG

Plasmid

#120228

Purpose

pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.

Depositor

Christopher Reisch

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9-NG

Use

Synthetic Biology

Mutation

Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…

Promoter

pTet

Available since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG-SsAPOBEC3B

Plasmid

#198536

Purpose

Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Publication

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-SsAPOBEC3B

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6d

Plasmid

#215822

Purpose

Express CBE6d (with SpCas9) in mammalian cells

Depositor

David Liu

Publication

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CBE6d-SpCas9-2xUGI (cas9 )

Expression

Mammalian

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-eSpCas9_sgAAVS1

Plasmid

#199213

Purpose

All-in-one plasmid encoding eSpCas9 and sgRNA targeting the AAVS1 site in human cells.

Depositor

Gabor Balazsi

Publication

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AAVS1-gRNA

Use

CRISPR

Promoter

human U6

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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Mammalian_SpCas9_Target-AID3S

Plasmid

#188649

Purpose

Expresses SpCas9_Target-AID3S in mammalian cells

Depositor

Keiji Nishida

Publication

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nCas9-AID3S-UGI

Tags

UGI

Expression

Mammalian

Mutation

D10A for SpCas9

Promoter

pCMV

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-QF#7

Plasmid

#46125

Depositor

Christopher Potter

Publication

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7

Expression

Insect

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pCasper4-QF#7-hsp70

Plasmid

#46135

Depositor

Christopher Potter

Publication

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7

Expression

Insect

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SpCas9-CBE6c-V106W

Plasmid

#215826

Purpose

Express CBE6c V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Publication

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CBE6c V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pRha-ABE8e-SpCas9-NG

Plasmid

#201190

Purpose

Expresses the base editor ABE8e-SpCas9-NG in bacterial cells

Depositor

Jonathan Yen

Publication

Mayuranathan et al Nat Genet. 2023 Jul;55(7):1210-1220. doi

Insert

ABE8e-SpCas9-NG

Tags

8xHIS and BP-NLS

Expression

Bacterial

Promoter

pRhaBAD

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g10

Plasmid

#80793

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

Depositor

Yonglun Luo

Publication

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9 ABE-Umax

Plasmid

#222138

Purpose

Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Publication

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9 (cas9 Synthetic)

Use

CRISPR; Zebrafish expression

Tags

bipartite NLS

Promoter

T3

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-SpCas9-2A-GFP-noITR

Plasmid

#118415

Purpose

Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

Depositor

Mark Denham

Publication

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SpCas9-CBE6d-V106W

Plasmid

#215827

Purpose

Express CBE6d V106W (with SpCas9) in mammalian cells

Depositor

David Liu

Publication

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CBE6d V106W-SpCas9-2xUGI (cas9 )

Use

In vitro transcription template

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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NLS-SpCas9mSA-His

Plasmid

#182951

Purpose

Express SpCas9mSA in bacteria

Depositor

Wen Xue

Publication

Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

Insert

NLS-SpCas9mSA-His

Use

CRISPR

Expression

Bacterial

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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G1397 DddAtox-N–dSpCas9

Plasmid

#157835

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Publication

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

bpNLS–G1397 DddAtox-N–dSpCas9–UGI–UGI–bpNLS

Use

CRISPR

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-deSpCas9

Plasmid

#92114

Purpose

Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Publication

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dead/inactive eSpCas9 with FLAG tag

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

D10A, H840A, K848A, K1003A, R1060A

Promoter

Cbh

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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