We narrowed to 5,829 results for: pCas

1. pSpCas9 (PX165)

   Plasmid

   #48137

   Purpose

   Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas9-polyA

   Plasmid

   #72602

   Purpose

   express hCas9 byT7 promoter with polyadenine tails

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

   Insert

   Cas9-polyadenine
   

   Tags

   81-bp polyadenylation

   Promoter

   T7

   Available Since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-DsRed2

   Plasmid

   #15777

   Depositor

   Edward Callaway

   Article

   Wickersham et al Neuron. 2007 Mar 1. 53(5):639-47.

   Insert

   DsRed2
   

   Expression

   Mammalian

   Available Since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAD29_hIRG1_4-461_pvp008

   Plasmid

   #124843

   Purpose

   Expresses human CAD (Irg1) in E.coli

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (ACOD1 Human)
   

   Tags

   StrepTag and TEV site

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-3 and 462-end.

   Promoter

   T7

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG/ATP9A-HA

   Plasmid

   #209213

   Purpose

   Mammalian expression of ATP9A

   Depositor

   Hye-Won Shin

   Article

   Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

   Insert

   ATP9A (ATP9A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-rtTA

   Plasmid

   #163601

   Purpose

   Expresses rtTA in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   rtTA
   

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-luciferase

   Plasmid

   #55764

   Purpose

   luciferase reporter for in vivo bioluminescence imaging

   Depositor

   Snorri Thorgeirsson

   Article

   Lee et al Cancer Res. 2010 Nov 1;70(21):8264-9. do

   Insert

   CMV enhancer/chicken beta actin promoter
   

   Expression

   Mammalian

   Promoter

   CMV enhancer/chicken beta actin promoter

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. dSpCas9-VPR

   Plasmid

   #205247

   Purpose

   In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.

   Depositor

   Rasmus Bak

   Article

   Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

   Insert

   dSpCas9-VPR
   

   Use

   CRISPR

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCambia-PUP-IT

   Plasmid

   #186478

   Purpose

   Expresses POI-PUP-IT fusion in Plants

   Depositor

   Jen Sheen

   Article

   Ye et al Nature. 2022 Sep 14. pii: 10.1038/s41586

   Insert

   FLAG-Pup(E)
   

   Tags

   Triple Flag Tag

   Expression

   Plant

   Promoter

   35S

   Available Since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-Flpe

    Plasmid

    #13787

    Purpose

    Mammalian expression of enhanced form of Flp recombinase with C-terminal Myc tag

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Insert

    Flpe
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9-CBE6b

    Plasmid

    #215820

    Purpose

    Express CBE6b (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6b-SpCas9-2xUGI (cas9 )
    

    Expression

    Mammalian

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-CFP

    Plasmid

    #11179

    Purpose

    Mammalian expression vector for expression of CFP

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

    Insert

    ECFP from Aequorea victoria
    

    Expression

    Mammalian

    Available Since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-SnoopCatcher

    Plasmid

    #72322

    Purpose

    Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a-SnoopCatcher
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed G842T and D848G of S. pneumoniae adhesin …

    Promoter

    T7

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. 2X_pX458_pSpCas9(BB)-2A-GFP

    Plasmid

    #172221

    Purpose

    Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-Fab_8D3_2_H-H6

    Plasmid

    #176075

    Purpose

    Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    His6 tagged heavy chain of Fab_8D3_2
    

    Tags

    His tag

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Fab_8D3_2_L

    Plasmid

    #176076

    Purpose

    Express light chain of Fab_8D3_2 in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    Light chain of Fab_8D3_2
    

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-Brainbow3.0

    Plasmid

    #45176

    Depositor

    Joshua Sanes

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Insert

    pCAG-Brainbow3.0
    

    Expression

    Mammalian

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCasAb-apr

    Plasmid

    #121998

    Purpose

    Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas9-Geminin

    Plasmid

    #109401

    Purpose

    Expresses Cas9 fusion to Geminin degron domain

    Depositor

    Jean-Paul Concordet

    Article

    Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

    Insert

    Geminin (GMNN Human)
    

    Mutation

    Geminin degron fragment

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAP03-acc(3)IV

    Plasmid

    #69862

    Purpose

    pCAP03-acc(3)IV is a upgraded version of the pCAP01(Plasmid #59981)

    Depositor

    Bradley Moore

    Article

    Tang et al ACS Chem Biol. 2015 Oct 21.

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Nov. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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