We narrowed to 4,380 results for: 1-Oct;

1. lentiCRISPR v2-Nr4a1sgRNA2

   Plasmid

   #160946

   Purpose

   Guide RNA 2 to generate Nr4a1 knockout by CRISPR

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Nr4a1 gRNA 2 (Nr4a1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-nAChr-Alpha6-CFP

   Plasmid

   #50482

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Tags

   CFP fusion in M3-M4 loop (after residue A405)

   Expression

   Mammalian

   Mutation

   Gly-Ala-Gly flexible linker flanking the CFP open…

   Promoter

   CMV

   Available Since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

3. TFORF2275

   Plasmid

   #142006

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SLC22A1 (SLC22A1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2276

   Plasmid

   #142007

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SLC22A1 (SLC22A1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF2274

   Plasmid

   #142005

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SLC22A1 (SLC22A1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBK-CMV-hNHERF2

   Plasmid

   #47801

   Purpose

   Expresses human NHERF2 in mammalian cells

   Depositor

   Randy Hall

   Article

   Lau et al Biochemistry. 2001 Jul 24;40(29):8572-80

   Insert

   NHERF2 (NHERF2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX303-ZIM3-KRAB-dCas9

   Plasmid

   #154472

   Purpose

   Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ZIM3 (ZIM3 Synthetic)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Includes ZIM3 aa 1-100

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.1 (-) + codon optimized Brn3a

   Plasmid

   #86829

   Purpose

   mammalian expression of the transcription factor Brn3a

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   Brn3a (POU4F1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEmerald-CD81-10

   Plasmid

   #54031

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   He et al PLoS Pathog. 2013;9(10):e1003701. doi: 1

   Insert

   CD81 (CD81 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. CMV-mGFP-pA(AV222)

    Plasmid

    #117858

    Purpose

    This plasmid expresses membrane anchored GFP to facility axon morphology study.

    Depositor

    C. Ron Yu

    Article

    Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

    Insert

    mGFP
    

    Use

    Adenoviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCold-TF-hPLEKHM1

    Plasmid

    #64146

    Purpose

    For protein expression in E.coli

    Depositor

    Tamotsu Yoshimori

    Article

    Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

    Insert

    PLEKHM1 (PLEKHM1 Human)
    

    Tags

    6xHis-tag and Trigger Factor (TF)

    Expression

    Bacterial

    Promoter

    cspA

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

    Plasmid

    #121944

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    U6 promoters

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX303-ABI1-dCas9

    Plasmid

    #154474

    Purpose

    Expresses ABI1 fused to the N terminus of dCas9

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ABI1 (ABI1 Mustard Weed)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Includes ABI1 aa 126-423, D143A

    Promoter

    CMV

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. N-Myc-53BP1 WT pLPC-Puro

    Plasmid

    #19836

    Depositor

    Titia de Lange

    Article

    Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

    Insert

    p53 Binding Protein 1 (TP53BP1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Available Since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDJSVT86

    Plasmid

    #108965

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al J Biol Chem. 2017 Dec 8;292(49):20240-20

    Type

    Empty backbone

    Tags

    OmpA 1-27, 6His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-UBE2E1 C131A in pcDNA3.1

    Plasmid

    #118214

    Purpose

    Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    UBE2E1 (UBE2E1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed cysteine 131 to alanine

    Available Since

    Oct. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28b-LanCL2 (6xHis-LanCL2)

    Plasmid

    #73407

    Purpose

    Expresses His tagged LanCL2

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    LanCL2 (LANCL2 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. p3×FLAG-CMV-14-LanCL2

    Plasmid

    #73391

    Purpose

    Expresses FLAG tagged LanCL2

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    LanCL2 (LANCL2 Human)
    

    Tags

    3x FLAG tagged

    Expression

    Mammalian

    Available Since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC57-GSX2-tTA-BleoR

    Plasmid

    #161748

    Purpose

    Tet-ON tTA plasmid for the c-terminal targeting of human GSX2 locus

    Depositor

    Elena Cattaneo

    Article

    Besusso et al Stem Cell Res. 2020 Dec;49:102016. doi:

    Insert

    Advanced tTA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    endogenous GSX2 promoter

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. KG#59

    Plasmid

    #110929

    Purpose

    Expresses proteins pan-neuronally in C. elegans (entire nervous system)

    Depositor

    Kenneth Miller

    Article

    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

    Inserts

    rab-3 promoter
    unc-54 3' control region
    

    Expression

    Bacterial

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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