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Plasmid#214682PurposeLentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)DepositorInsertdgRNA_Chr1
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Venus_hs_NC_chr2
Plasmid#214683PurposeLentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 2 (negative control)DepositorInsertdgRNA_Chr2
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Venus_hs_TP73
Plasmid#214685PurposeLentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human TP73DepositorInsertdgRNA_TP73 (TP73 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_BFP_hs_NC_chr1
Plasmid#214686PurposeLentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)DepositorInsertdgRNA_Chr1
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_BFP_hs_TP73
Plasmid#214687PurposeLentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human TP73DepositorInsertdgRNA_TP73 (TP73 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOliSpec5'
Plasmid#228551PurposeTemplate for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmidDepositorInsertTruncated 5' region of aadA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOliSpec3'
Plasmid#228550PurposeTemplate for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.DepositorInsertTruncated 3' region of aadA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE-miRFP703_PGK-Puro
Plasmid#216667PurposeExpresses tTa-inducible miRFP703, with puromycin resistanceDepositorInsertsmiRFP703
PuroR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti puro HA-TUBA1B
Plasmid#227587PurposeLentiviral plasmid expressing human TUBA1B proteinDepositorInsertTUBA1B (TUBA1B Human)
UseLentiviralTagsHAExpressionMutationPromoterCMVAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-phSyn-flex-SypHaloTag
Plasmid#172280PurposeCre-dependent HaloTag labeling of neuronal presynaptic terminals from the synapsin promoter.DepositorInsertsynaptophysin-HaloTag
UseAAV and Cre/LoxTagsHaloTagExpressionMammalianMutationPromoterrat synapsinAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
MDH2_EC
Plasmid#224146PurposeIPTG induces expression of C. elegans protein MDH-2 with a N-terminal His tag.DepositorInsertMDH-2 (mdh-2 Nematode)
UseTagsHis tag, Xpress tag, enterokinase cleaveage siteExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIND4-GFP-MmXYRS
Plasmid#222745PurposeExpresses sfGFP and aaRS-tRNA pair for incorporating haloalkane crosslinkerDepositorInsertssfGFP
MmXYRS
Mm Pyl tRNA
UseTags6xHisExpressionMutationContains the following substitutions: V346A, W348…PromoterrrnBAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p1-GST-Ubiquitin
Plasmid#223236PurposeBacterial expression construct for GST-Tagged Ubiquitin production and purificationDepositorInsertUbiquitin
UseTagsExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
H2B-Electra2
Plasmid#218929PurposeElectra2 fused to human H2B protein for nuclear localizationDepositorInsertH2B-Electra2 (H2B Entacmaea quadricolor, Synthetic, Human)
UseSynthetic BiologyTagsElectra2 and H2BExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
LvPolyUb
Plasmid#218982PurposeL.variegatus polyubiquitin-C promoterDepositorInsertL.variegatus polyubiquitinC promoter
UseSea urchinTagsExpressionMutationPromoterL.variegatus polyubiquitinC promoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pet28a+_Yjjg
Plasmid#220228PurposeBacterial expression of Yjjgin E. coli BL21_DE3 (pLys) or similarDepositorInsertPyrimidine 5'-nucleotidase YjjG (yjjG E.coli)
UseTagsHistagExpressionBacterialMutationPromoterT7Available sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-spacer-H1
Plasmid#218399Purpose3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
MUT_S1-H1,NR
Plasmid#218395Purpose3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice siteDepositorInsertModified CHO DHFR minigene
UseTagsExpressionMammalianMutationPromotertetracycline-responsive promoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
1167A
Plasmid#218233PurposeHDR plasmid for inserting Ceratitis capitata transformer female intron into the white pupae geneDepositorInsertputative metabolite transport protein
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Flag-RBM24
Plasmid#208621Purposefor E. coli expression of Flag tagged human RBM24 (ENST00000379052)DepositorInserthuman RBM24 (ENST00000379052) coding region (RBM24 Human)
UseTags6xHis and FlagExpressionBacterialMutationPromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only