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LRT_RTctrl

Plasmid

#214074

Purpose

Luminescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

Firefly luciferase

Expression

Mammalian

Mutation

Linker sequence present between firefly luciferas…

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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LRT

Plasmid

#214071

Purpose

Luminescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

Firefly luciferase

Expression

Mammalian

Mutation

Linker sequence present between firefly luciferas…

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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LRTX_ctrl

Plasmid

#214073

Purpose

Luminescent reporter system, NMD negative control. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

Firefly luciferase

Expression

Mammalian

Mutation

Remnant of linker sequence present at the end of …

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTPH412

Plasmid

#185724

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-gp41-8N

Use

CRISPR

Expression

Bacterial

Mutation

ecTadA(8e) R26G

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-SH3BP1

Plasmid

#136647

Purpose

Expression of SHBP1-GST fusion protein in E. coli

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1 (SH3BP1 Human)

Expression

Bacterial

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pscALPS_puro

Plasmid

#128504

Purpose

Encodes puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

Puromycin Resistance

Use

Lentiviral

Expression

Mammalian

Promoter

Cyclophilin A promoter

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTn7xTS-mPlum

Plasmid

#117392

Purpose

Tn7 tagging vector pTn7xTS with mPlum expression scaffold

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

mPlum

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available Since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-PXN

Plasmid

#112957

Purpose

In vivo visualization of focal adhesions (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

PXN

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDONRPEX18Km-SceI-pheS

Plasmid

#111610

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Article

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pSBDEST.N

Plasmid

#79468

Purpose

Promoterless, Sleeping Beauty-compatible Gateway destination vector, Neomycin

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Type

Empty backbone

Use

Promoterless gateway destination vector

Promoter

none

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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RCAS(A) mPax1 (CT#1039)

Plasmid

#13902

Depositor

Cliff Tabin

Article

Brent et al Cell. 2003 Apr 18. 113(2):235-48.

Insert

Pax1 (Pax1 Mouse)

Use

Retroviral; Avian expression

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pDONR223 SARS-CoV2 TEV-ORF3A

Plasmid

#154416

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF3A (ORF3a SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of o…

Mutation

Many synonymous changes due to codon optimization

Available Since

Sept. 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV2 TEV-NSP12

Plasmid

#154410

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of n…

Mutation

Many synonymous changes due to codon optimization

Available Since

July 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV2 TEV-NSP1

Plasmid

#154400

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector, with insert of n…

Mutation

Many synonymous changes due to codon optimization

Available Since

July 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 NSP5 Cys146Ala_nostop

Plasmid

#154030

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP5

Use

Gateway-compatible entry vector, with mutagenized…

Mutation

Many synonymous changes due to codon optimization

Available Since

June 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3 PM-Ras-LOCKR-S

Plasmid

#216498

Purpose

FRET-based reporter for Ras activity, plasma membrane localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

PM-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pOGG001

Plasmid

#113978

Purpose

Level 0 module. nptII promoter for constitutive gene expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

nptII promoter

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-rxRFP1.2

Plasmid

#67839

Purpose

redox-sensitive red fluorescent protein rxRFP1.2

Depositor

Huiwang Ai

Article

Fan et al Anal Bioanal Chem. 2016 Jan 12.

Insert

rxRFP1.2

Tags

His Tag

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
KIF5C(1-560)-mCitrine

Plasmid

#61676

Purpose

Single mCitrine-labeled, truncated kinesin-1

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KHC(1-560)-mCitrine (Kif5c Rat)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pBY2807

Plasmid

#23222

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

Gateway-SnAvi

Expression

Bacterial, Insect, and Mamm…

Available Since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only