We narrowed to 4,564 results for: HRE
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Plasmid#224568PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)ExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
FoxO1 KR
Plasmid#66166PurposeExpresses FoxO1 with three Lys residues surrounding Ser-253 that are known to be sites of acetylation were replaced by arginine residues.DepositorInsertFoxO1 KR (Foxo1 Mouse)
UseRetroviralTagsExpressionMammalianMutationK242R, K245R and K262RPromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP-EGFP-mouse Plaat3 (WT)
Plasmid#167987PurposeExpresses EGFP-Plaat3 in mammalian cellsDepositorInsertHrasls3 (Plaat3 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
FoxO1 KA
Plasmid#66164PurposeExpresses FoxO1 with three Lys residues surrounding Ser-253 that are known to be sites of acetylation were replaced by alanine residues.DepositorInsertFoxO1 KA (Foxo1 Mouse)
UseRetroviralTagsExpressionMammalianMutationK242A, K245A and K262APromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
FoxO1 KQ
Plasmid#66165PurposeExpresses FoxO1 with three Lys residues surrounding Ser-253 that are known to be sites of acetylation were replaced by glutamine residues.DepositorInsertFoxO1 KQ (Foxo1 Mouse)
UseRetroviralTagsExpressionMammalianMutationK242Q, K245Q and K262QPromoterAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3
Plasmid#62957PurposeTriplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.DepositorInsertT2A-Gal4 in three phases
UseTagsExpressionMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
AlkB-D135T
Plasmid#160433PurposeA plasmid for expression in E. coli to produce His-tag fusion protein AlkB with Asp at position 135 mutated to ThrDepositorInsertE. coli AlkB
UseTagsHis-tagExpressionBacterialMutationChanged Aspartic acid 135 to Threonine; deleted t…PromoterT7Available sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSF107mT-GATEWAY-3'-3HA
Plasmid#67616PurposeA pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technologyDepositorTypeEmpty backboneUseIn vitro transcription or translation off of an s…Tagsthree inframe 3' HA tagsExpressionMammalianMutationPromoterCMV and SP6Available sinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
Plasmid#159849PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
XE118 CAMKII-T286D-CS2+
Plasmid#16736DepositorInsertCAMKII-T286D (Camk2a Rat)
UseXenopus expressionTagsExpressionMutationthreonine 286 mutated to aspartatePromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLEG(R1-R3) #BGV149
Plasmid#48956PurposeLentiviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
AMPK(T>A)-SPARK
Plasmid#226256Purposephospho-null mutant of AMPK-SPARKDepositorInsertAMPK(T>A)-SPARK
UseTagsExpressionMammalianMutationsubstituted Threonine 20 with AlaninePromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
p CIneo-RL-Let7-3xBulgeB-mut
Plasmid#115369PurposeExpression vector to produce humanized Renilla luciferase with three mutated (seed-sequence mutations) partially complementary binding sites for human let-7 miRNA in the 3'UTRDepositorInsertRenilla Luciferase
UseLuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
YFP-Cdc42(N17)
Plasmid#11400DepositorInsertcell division cycle 42 (CDC42 Human)
UseTagsYFPExpressionMammalianMutationObtained with Threonine 17 changed to Asparagine.PromoterAvailable sinceFeb. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd g4+g10+g18 (FWA)
Plasmid#115487PurposeCRISPR Cas9 SunTag system to target NtDRMcd to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-RQ/TG
Plasmid#25369DepositorInsertLRRK2 (LRRK2 Human)
UseTags2XMycExpressionMammalianMutationThreonine 1343 mutated to Glycine Arginine 139…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
FLAG-OTUB1 T134R-pcDNA3.1
Plasmid#118211PurposeExpresses FLAG-OTUB1 T134R mutant in mammalian cellsDepositorInsertOTUB1 (OTUB1 Human)
UseTags3x FLAGExpressionMammalianMutationchanged threonine 134 to argininePromoterAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1V5-His Snk/Plk2 T239D
Plasmid#22220DepositorInsertSnk/Plk2 (PLK2 Human)
UseTagsExpressionMammalianMutationsingle amino acid substitution mutant, threonine …PromoterAvailable sinceAug. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
YFP-Rac1(N17)
Plasmid#11395DepositorInsertras-related C3 botulinum toxin substrate 1 (RAC1 Human)
UseTagsYFPExpressionMammalianMutationObtained with Threonine 17 mutated to Asparagine.PromoterAvailable sinceFeb. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-Xyl-DocI
Plasmid#58711PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertXyl-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationchanged Threonin 120 to Cysteine in Xylanase Doma…PromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only