We narrowed to 9,088 results for: ef1

Showing: 3981 - 4000 of 9088 results

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AsdCas12a KRAB

Plasmid

#188502

Purpose

Expresses FLAG tagged AsdCas12a KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D908A

Promoter

EF1a

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-EAMP

Plasmid

#138265

Purpose

mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.

Depositor

Xiao Wang

Article

Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

Insert

iCas9 target-mCherry

Use

CRISPR

Expression

Mammalian

Promoter

EF1a-HTLV

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-RT003-GPM

Plasmid

#163383

Purpose

To express sGFP module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

GFP-PDGFRtm-2A-mCherry (GPM)

Use

Lentiviral

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

EF1a

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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SaCas9

Plasmid

#188494

Purpose

Expresses FLAG tagged SaCas9

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-dAPEX2

Plasmid

#117173

Purpose

Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

dAPEX2

Use

AAV

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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alpha7nACHR_Nacho_mCherry

Plasmid

#182939

Purpose

alpha7 nACHR expression in mammalian cell lines

Depositor

Robert Brenner

Article

Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

Insert

NACH7 (CHRNA7 Human)

Use

Synthesized

Expression

Mammalian

Promoter

EF1alpha

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pWPXL-Sox9

Plasmid

#36979

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 6, 2012

Availability

Academic Institutions and Nonprofits only

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pAC1749

Plasmid

#127983

Purpose

AMA1 plasmid with Aspergillus optimized Cpf1, hygB selection marker, and USER cassette (PacI/Nt.BbvCI)

Depositor

Uffe Mortensen

Article

Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

Insert

Cpf1

Use

CRISPR; Fungal expression

Tags

SV40 NLS

Expression

Bacterial

Mutation

codon optimized for Aspergillus nidulans

Promoter

Aspergillus nidulans tef1 promoter

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEmpty_Control

Plasmid

#162607

Purpose

Empty control for Cas9 experiments and to clone new guide RNAs for paper

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-RT001-LVB

Plasmid

#163381

Purpose

Second generation lentiviral vector to express anti-GFP(LaG17) module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

LaG17-VEEGFRtm-BFP (LVB)

Use

Lentiviral

Tags

BFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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PB-CT3G-ERP2

Plasmid

#175497

Purpose

All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE3G-mCMV

Available Since

Oct. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-MCS-3AID-HA-2A-mCherryBSD

Plasmid

#225700

Purpose

Empty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinR

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Tags

T2A-mCherry

Expression

Mammalian

Promoter

pEF1A

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-NLS-dPguCas13b-3xmRuby3-NLS

Plasmid

#132406

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPguCas13b

Use

Lentiviral

Expression

Mammalian

Mutation

H151A; H1121A

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV SOCS-3

Plasmid

#11486

Depositor

Ronald Kahn

Article

Ueki et al Mol Cell Biol. 2004 Jun . 24(12):5434-46

Insert

SOCS-3 (Socs3 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pLuc-3'UTR

Plasmid

#42094

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

ZEB1 3UTR (ZEB1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pFUSE-rabbitFc1-IL2ss-VHH(G08)-His-Myc

Plasmid

#209857

Purpose

Plasmid coding for a nanobody (termed G08) against FGFR3 (mus musculus) with x6 HisTag, x3 myc, and Fabbit FC gamma 1

Depositor

Tim Brown

Inserts

IL2-ss
VHH (G08 variant)
Rabbit Fc Gamma 1

Tags

x3 MycTag and x6 HisTag

Expression

Mammalian

Promoter

hEF1-HTLV promoter

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEBB HA cIAP1

Plasmid

#38232

Depositor

Colin Duckett

Article

Csomos et al Biochem J. 2009 Apr 28;420(1):83-91.

Insert

cIAP1 (BIRC2 Human)

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

July 20, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) MID51 4xMyc Hisx6

Plasmid

#44598

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

MiD51 (Mief1 Mouse)

Tags

4xMyc and 6xHis

Expression

Mammalian

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_lbMicroID2-HA

Plasmid

#188522

Purpose

Encodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cells

Depositor

James Londino

Article

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

lbMicroID2

Use

Transposon mediated gene integration

Tags

HA

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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EdTP

Plasmid

#24311

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

EF1alpha-dnhTcf4 // SV40-PuroR (TCF7L2 Human)

Use

Lentiviral

Tags

FLAG

Mutation

31 first amino acids of hTcf4 have been deleted.

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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