We narrowed to 24,450 results for: ina

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M3D395A-M14-dCas9

Plasmid

#134780

Purpose

Expression of fusion protein M3D395A-M14-dCas9 in Mammalian cells

Depositor

Shu-Bing Qian

Article

Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

Insert

METTL3/METTL14 methyltransferase domain and inactive dCas9

Expression

Mammalian

Mutation

D395A

Promoter

CMV

Available Since

Feb. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5-hPPT1

Plasmid

#85815

Purpose

Expression of hPPT in mammalian cells

Depositor

Sandra Hofmann

Article

Lu et al Mol Genet Metab. 2010 Apr;99(4):374-8. d

Insert

human PPT1 (PPT1 Human)

Expression

Mammalian

Available Since

Jan. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2 Chfr (1014)

Plasmid

#61853

Purpose

mammalian expresion of Chfr fused to EGFP

Depositor

Jonathon Pines

Article

Matsusaka et al J Cell Biol. 2004 Aug 16;166(4):507-16.

Insert

Chfr (CHFR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only
Dead Streptavidin-SpyTag

Plasmid

#59548

Purpose

pET21-DTag encodes Dead streptavidin (negligible biotin binding) bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin tetramers

Depositor

Mark Howarth

Article

Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

Insert

Dead Streptavidin-SpyTag

Tags

SpyTag

Expression

Bacterial

Mutation

The construct lacks final K of SpyTag

Promoter

T7

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
pDONR223-TRIB2

Plasmid

#23589

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TRIB2 (TRIB2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
LZRS-Mek2-K101A (KA7)

Plasmid

#21191

Depositor

Paul Khavari

Insert

Mek2 (MAP2K2 Human)

Use

Retroviral

Mutation

K101A (catalytically inactive)

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
pAG654 pFAST

Plasmid

#172861

Purpose

Expresses pFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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LAMP-mChF-AIP

Plasmid

#61524

Purpose

Expression of Homo sapiens lysosomal-associated membrane protein 1 (LAMP1) tagged with mCherry and AIP

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

lysosomal-associated membrane protein 1 (LAMP1 Human)

Tags

AIP and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Barkor

Plasmid

#24402

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Barkor (ATG14 Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only
pAd-PV-NES-DsRed

Plasmid

#17286

Depositor

Anton Bennett

Article

Rodrigues et al J Biol Chem. 2007 Jun 8. 282(23):17061-8

Insert

PV-NES (Pvalb Rat)

Use

Adenoviral

Tags

DSR and Myc

Expression

Mammalian

Available Since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only
ND4 left TALE–G1397 DddAtox N

Plasmid

#158094

Purpose

expresses the right-side ND4 TALE–DddAtox half in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

SOD2 MTS–3xHA–ND4 left TALE–G1397 DddAtox-N–1xUGI

Expression

Mammalian

Mutation

WT

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-eEF2K

Plasmid

#110160

Purpose

Expression of human eEF2K in mammalian cells

Depositor

Daniele Guardavaccaro

Article

Kruiswijk et al Sci Signal. 2012 Jun 5;5(227):ra40. doi:

Insert

eEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA P1

Plasmid

#80943

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA P1 for Pd-l1 binding.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCW-GFP-CtIP-T847E

Plasmid

#71111

Purpose

mammalian expession of GFP-CtlP T847E mutation

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

T847E

Promoter

TRE promoter, Tet ON

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE

Plasmid

#118273

Purpose

pAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoter

Depositor

Naoshige Uchida

Article

Menegas et al Nat Neurosci. 2018 Sep 3. pii: 10.1038/s

Insert

GCaMP6f

Use

AAV

Tags

6xHis (N terminal on insert), T7 epitope, and Xpr…

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCD5-H1MI15-CO-GCN4-TEV-sfGFP-TS

Plasmid

#158741

Purpose

Expresses influenza A virus trimeric hemagglutinin A/H1/MI/15 fused to a sfGFP

Depositor

Robert de Vries

Article

van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

Insert

Hemagglutinin A/MI/45/15 H1N1

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

AA61-469

Promoter

CMV

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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ND4 right TALE–G1397 DddAtox C

Plasmid

#158097

Purpose

expresses the right-side ND4 TALE–DddAtox half in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA tox-C–1xUGI

Expression

Mammalian

Mutation

WT

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pKK-TEV-mClover3

Plasmid

#105795

Purpose

Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-mClover3

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pQHA-USP11 CS puroR

Plasmid

#46750

Purpose

Retroviral vector that expresses catalytically inactive form of HA-tagged human USP11

Depositor

Goedele Maertens, Gordon Peters

Article

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP11 (USP11 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C318S--catalytically inactive

Promoter

CMV

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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Lenti-Cre

Plasmid

#198712

Purpose

For lentiviral expression of Cre recombinase in mammalian cells.

Depositor

Tyler Jacks

Article

Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

Insert

Pgk-Cre

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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