We narrowed to 24,461 results for: nes

1. pSLIK_Zeo_3xflag_Luciferase

   Plasmid

   #136533

   Purpose

   Lentiviral expression vector for an inducible 3xflag-tagged Luciferase

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   Luciferase
   

   Use

   Lentiviral; Destinatioin vector for gateway cloni…

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGB Pnos:Luciferase:Tnos (GB0112)

   Plasmid

   #68217

   Purpose

   Transcriptional unit for Firefly Luciferase Protein plant expression driven by the Nos promoter

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Luc
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-3xflagGREB1b

   Plasmid

   #112222

   Purpose

   mammalian expression of flag tagged GREB1b

   Depositor

   Craig Burd

   Article

   Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

   Insert

   GREB1b (GREB1 Human)
   

   Use

   Tags

   3x flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEN_TT Tgfbr3-HA

   Plasmid

   #58497

   Purpose

   Gateway entry vector for an inducible HA-tagged mouse Tgfbr3

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

   Insert

   Tgfbr3_HA (Tgfbr3 Mouse)
   

   Use

   Entry vector

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2014

   Availability

   Academic Institutions and Nonprofits only

5. hCMV-IE1:Lucia:bgH

   Plasmid

   #135293

   Purpose

   Transcriptional unit for constitutive expression of Lucia luciferase in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:Lucia:bGH
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSU314

   Plasmid

   #169696

   Purpose

   Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.

   Depositor

   Nara Figueroa

   Article

   Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

   Insert

   1xHA epitope tag adjacent to FRT-flanked cat (CamR) gene
   

   Use

   Tags

   1xHA tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOpLexA::mini35s (GB0733)

   Plasmid

   #68180

   Purpose

   Provides the 4xLexA Operator fused to CaMV 35s minimal promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   4xOpLexA:mini35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXylA-RgTALsyn

   Plasmid

   #100948

   Purpose

   Expression of Tyrosine Ammonia Lyase from constitutive XylA promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Insert

   RgTALsyn
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   XylA (Constitutive)

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGB1omega2_SF (GB0466)

   Plasmid

   #68243

   Purpose

   Twister plasmid to swap inserts from an omega1 or omega1R vector to any alpha level vector

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Solanum lycopersicum intergenic region
   

   Use

   Synthetic Biology; Unspecified

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGB1alpha2_SF (GB0107)

    Plasmid

    #68233

    Purpose

    Twister plasmid to swap inserts from an alpha1 or alpha1R vector to any omega level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSU312

    Plasmid

    #169694

    Purpose

    Template plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    1xFLAG epitope tag adjacent to FRT-flanked aph (KanR) gene
    

    Use

    Tags

    1xFLAG tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSU313

    Plasmid

    #169695

    Purpose

    Template plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    1xFLAG epitope tag adjacent to FRT-flanked cat (CamR) gene
    

    Use

    Tags

    1xFLAG tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-HA-mIFITM1-C49,50,83,103A

    Plasmid

    #58421

    Purpose

    Expresses murine IFITM1-C49,50,83,103A with an N-terminal HA tag in mammalian cells (cysteine-less mutant that is not palmitoylated).

    Depositor

    Jacob Yount

    Article

    Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

    Insert

    IFITM1 (Ifitm1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Cysteines 49, 50, 83, and 103 to Alanine

    Promoter

    CMV IE

    Available since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

14. AAV-CAG-DIO-mAPX

    Plasmid

    #79908

    Purpose

    Adeno-associated virus plasmid, Cre-dependent FLEX switch, mAPX

    Depositor

    Joshua Sanes

    Article

    Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

    Insert

    mAPX
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTHsp18.2 (GB0035)

    Plasmid

    #68186

    Purpose

    Provides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtHsp18.2 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBBK12 pCAS-Tyr-[gRNA: 2=FF21] (SplitKanR, AmpR)

    Plasmid

    #178996

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF21 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)

    Plasmid

    #178995

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Nano-lantern-H2B/pcDNA3

    Plasmid

    #51971

    Purpose

    mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to Histone H2B

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern
    

    Use

    Tags

    human H2B

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pOpLacI:mini35ss (GB0534)

    Plasmid

    #68179

    Purpose

    Provides the 6xLacI Operon fused to CaMV 35S minimal promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    6xOpLacI:mini35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. A337C

    Plasmid

    #24432

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C

    Promoter

    Available since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only