We narrowed to 34,848 results for: CaS;

1. zCREST3:Cas9green; EGF_gRNA

   Plasmid

   #199333

   Purpose

   pDEL158; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 EGF sgRNA

   Depositor

   William Talbot

   Article

   Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

   Inserts

   zCREST3
   Cas9
   nrg1 EGF sgRNA
   

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFREE2-SpCas9-sgRNA

   Plasmid

   #179582

   Purpose

   Expresses spCas9 and gRNA that targets ColE1 plasmids for curing

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-AaCas12b-2AeGFP

   Plasmid

   #121945

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AaCas12b
   

   Use

   Tags

   3xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pShoCAST-sgRNA_entry (BO1)

   Plasmid

   #181786

   Purpose

   Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac and J23119

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. RCAS(B)-Flag-PLZF

   Plasmid

   #34887

   Depositor

   Peter Vogt

   Article

   Shi et al Int J Cancer. 2009 Oct 1;125(7):1558-65.

   Insert

   PLZF (PLZF, ZBTB16 Human)
   

   Use

   Retroviral; Avian expression

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pRS415_pGal-nCas9(H840A)

   Plasmid

   #79616

   Purpose

   Expresses nCas9(H840A) in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H840A for nickase Cas9

   Promoter

   pGal1

   Available Since

   Sept. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMBP-LbCas12a D832A

   Plasmid

   #113517

   Purpose

   Expresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nuclease

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   LbCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   D832A

   Promoter

   T7

   Available Since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAT15547_PEmax-SuperFi-Cas9

   Plasmid

   #184377

   Purpose

   Mammalian expression of SuperFi-Cas9 PEmax prime editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   PEmax-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS and c-myc NLS

   Expression

   Mammalian

   Mutation

   H840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V101…

   Promoter

   CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUGW Cas9-BFP

   Plasmid

   #127396

   Purpose

   Cas9 expression

   Depositor

   Xiaowei Zhuang

   Article

   Wang et al Proc Natl Acad Sci U S A. 2019 May 28;11

   Insert

   Cas9
   

   Use

   Lentiviral

   Tags

   BFP

   Expression

   Mutation

   Promoter

   SVVF

   Available Since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. beta2 (px330:2-guideCas9)

    Plasmid

    #169435

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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