We narrowed to 7,531 results for: Ski

1. pHS1861

   Plasmid

   #205974

   Purpose

   FLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas8-HNH
   

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-MBP-TEVpCS-ADGRL2 CTF-FLAG

   Plasmid

   #217707

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRL2 (ADGRL2 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-824

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-5X1 Flag hDPY30

   Plasmid

   #15556

   Depositor

   Kai Ge

   Article

   Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

   Insert

   DPY30 (DPY30 Human)
   

   Tags

   Flag and GST

   Expression

   Bacterial

   Available Since

   Aug. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pGEX Stat5B-SH2

   Plasmid

   #46516

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Stat5B (STAT5B Human)
   

   Tags

   AviTag, GST, and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 593-692

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. PVT1-luciferase

   Plasmid

   #113350

   Purpose

   evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

   Depositor

   Ralf Jauch

   Article

   Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

   Insert

   PVT1-enhancer (PVT1 Human)
   

   Use

   Luciferase

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDule2-IBBN (G2)

   Plasmid

   #85501

   Purpose

   Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

   Insert

   IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Expression

   Bacterial

   Mutation

   Y32G L65E F108W Q109M D158S R162K

   Promoter

   lpp (constitutive)

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEY26

   Plasmid

   #191031

   Purpose

   cho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   cho-1(3.3-2.6k) (cho-1 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEY39

   Plasmid

   #191043

   Purpose

   acr-2(2.1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

   Depositor

   Oliver Hobert, Eviatar Yemini

   Article

   Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

   Insert

   acr-2(2.1k) (acr-2 Nematode)
   

   Expression

   Worm

   Mutation

   None

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-flex-GFP-shRNA-scramble

   Plasmid

   #182503

   Purpose

   Expresses scramble shRNA for control expts. Flexed cassette driven by pan neuronal gene promoter

   Depositor

   William Wisden

   Article

   Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

   Insert

   pan neuronal promoter driving flexed GFP-shRNA scramble from mir 30 cassette
   

   Use

   AAV, Cre/Lox, and RNAi

   Expression

   Mammalian

   Promoter

   hSyn promoter

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-51b(+)-Caprola_09

    Plasmid

    #194670

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_09 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_09
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMCL-HA-MAPKK1-G1C [delta(44-51)/S218E/S222D]

    Plasmid

    #40807

    Depositor

    Natalie Ahn

    Article

    Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta(44-51), S218E, S222D

    Promoter

    CMV

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA SF-HERC2 F1

    Plasmid

    #55784

    Purpose

    Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1-969aa)

    Depositor

    David Chan

    Article

    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

    Insert

    3xFLAG tagged HERC2 (1-969aa) (HERC2 Human)
    

    Tags

    3xFLAG and STREP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pCS2 Smad1 EVE GM

    Plasmid

    #27997

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    Human Smad1 EVE Gsk3 mutant (SMAD1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S463E S465E S210A T202A S191A S198A

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pRK5 GFP RagB Q99L

    Plasmid

    #112749

    Purpose

    express GFP-RagB (Q99L mutant)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    GFP-RagB (Q99L) (RRAGB Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Q99L

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX ShcD-SH2

    Plasmid

    #46488

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    ShcD (SHC4 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 512-623

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHS1543

    Plasmid

    #205963

    Purpose

    NZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    DinG HNH Cascade with His14-tagged Csf3
    

    Tags

    14xHis on Csf3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHS1871

    Plasmid

    #205976

    Purpose

    U6-JAGBLH010000220 Cas8-HNH DR-BpiI-DR

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH DR-BpiI-DR
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti CRISPR sgMTHFD2_1

    Plasmid

    #106313

    Purpose

    Express Cas9 and sgRNA targeting MTHFD2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD2 (MTHFD2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX SH2-B-SH2

    Plasmid

    #46479

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SH2-B (SH2B1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 515-639

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPHR-iRFP713

    Plasmid

    #103818

    Purpose

    Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    PHR-iRFP713 (CRY2 Mustard Weed)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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