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Plasmid#218197PurposeBacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF2DepositorInsertEndoF2
UseTagsCBD-HisExpressionBacterialMutationcodon optimized Ecoli, residues 45-335PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Prepro-3x-Beta-Amyloid
Plasmid#181730PurposeGalactose inducible expression of Prepro-3x-Beta-AmyloidDepositorInsertPrepro-3x-Beta-Amyloid
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Kar2-Beta-Amyloid
Plasmid#181707PurposeGalactose inducible expression of Kar2-Beta-AmyloidDepositorInsertKar2-Beta-Amyloid
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NI_G1333-DddA11-N
Plasmid#191609PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NN_G1333-DddA11-N
Plasmid#191610PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_HD_G1333-DddA11-C
Plasmid#191599PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NG_G1333-DddA11-C
Plasmid#191600PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NI_G1333-DddA11-C
Plasmid#191601PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NN_G1333-DddA11-C
Plasmid#191602PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_HD_G1333-DddA11-N
Plasmid#191607PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
E3_pENTR_L3-L2_NG_G1333-DddA11-N
Plasmid#191608PurposeptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
UseOtherTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1_polio_VP12A
Plasmid#212988PurposeEncodes Poliovirus type I Mahoney VP12A optimized for expression in mammalian transcription/translation extractsDepositorInsertPoliovirus type I Mahoney VP12A
UseMammalian cell free protein expressionTagsHA tagExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pNHA_OROV_NSs
Plasmid#208934PurposeExpresses N-terminally HA-tagged Oropouche orthobunyavirus NSs protein from CMV promoter in mammalian cellsDepositorInsertNSs
UseTagsHAExpressionMammalianMutationDeleted 69 bp at 5' end (including ATG) and …PromoterCMVAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-NPHP1
Plasmid#209769PurposeHuman NPHP1 (Nephrocystin 1) gene is inserted into pPB-PGK-destination (Plasmid #60436).DepositorInsertNPHP1 (NPHP1 Synthetic, Human)
UsePiggybac transposonTagsHA tagExpressionMammalianMutationPromoterCAGAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR8GW-HA-Wnk
Plasmid#203967PurposeHA-Wnk basic constructDepositorInsertWnk (most similar to RO variant) (Wnk Fly)
UseUnspecifiedTagsHAExpressionMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR3cDual-Fray
Plasmid#203977PurposeEnrty clone for Gateway cloningDepositorInsertFrayed (fray Fly)
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEXfrt-SaCas9-U6-sgNtsr1(guide_2)
Plasmid#205417PurposeMutagenesis of Ntsr1, second guideDepositorInsertNtsr1 (Ntsr1 Mouse)
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-POWV_NS2B-GS-NS3
Plasmid#203471PurposeExpresses POWV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 markerDepositorInsertPOWV NS2B-GS-NS3 (POLY )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-LGTV_NS2B-GS-NS3
Plasmid#203468PurposeExpresses LGTV NS2B-NS3 protease (with GS linker) from a GAL10 promoter with a URA3 markerDepositorInsertLGTV NS2B-GS-NS3 (POLY )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only