We narrowed to 9,116 results for: Ott

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6xHis-SNAP-PI(3,4,5)P3 Probe

Plasmid

#211514

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

BTK1-PH

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)

Plasmid

#71814

Purpose

Expresses high specificity SpCas9. Px330-like plasmid.

Depositor

Feng Zhang

Article

Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

Has Service

Cloning Grade DNA

Insert

enhanced specificity Cas9 (1.1)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

K848A, K1003A, & R1060A

Promoter

CBh

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-Vinculin-N-21

Plasmid

#55160

Purpose

Localization: Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Vinculin (VCL Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pET15b-RvLEAMshort

Plasmid

#218122

Purpose

Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

Depositor

Ci Ji Lim

Article

Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

Insert

RvLEAMshort

Tags

6xHIS

Expression

Bacterial

Mutation

Truncated form (58-181aa)

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Soma-jGCaMP8m

Plasmid

#239670

Purpose

Cre-dependent expression of Soma-jGCaMP8m under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8m

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV CaMKII:RiboL1-jGCaMP8s

Plasmid

#239667

Purpose

Expresses soma-targeted (ribosome tethered via RiboL1) jGCaMP8s under CaMKII promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8s

Use

AAV

Tags

6xHis and RiboL1

Expression

Mammalian

Promoter

CaMKII

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-RspCas13d

Plasmid

#108305

Purpose

Expresses E. coli codon optimized RspCas13d in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

RspCas13d

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_EmGFP

Plasmid

#113892

Purpose

Mammalian (inducible) expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

Emerald GFP

Use

Lentiviral

Promoter

CMV-MIE-TetO2

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-SNAP-tag

Plasmid

#167269

Purpose

Overexpression of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SNAP-tag

Tags

His x10

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-RiboL1-jGCaMP8s

Plasmid

#192611

Purpose

AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8s.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-Axon-jRex-GECO

Plasmid

#172714

Purpose

AAV transfer plasmid for neuronal expression of axon-targeted jRex-GECO.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Axon-jRex-GECO

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Soma-jGCaMP8s

Plasmid

#239669

Purpose

Cre-dependent expression of Soma-jGCaMP8s under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8s

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn:FLEX-Soma-jGCaMP8f

Plasmid

#239671

Purpose

Cre-dependent expression of Soma-jGCaMP8f under hSyn promoter.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

Soma-jGCaMP8f

Use

AAV

Tags

6xHis and EE-RR

Expression

Mammalian

Promoter

hSyn

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pY095

Plasmid

#84744

Purpose

Expresses huLbCpf1-T2A-GFP and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huLbCpf1
GFP

Use

CRISPR

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462)

Plasmid

#48141

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pY026

Plasmid

#84741

Purpose

Expresses huAsCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huAsCpf1

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pGGAselect

Plasmid

#195714

Purpose

pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 24, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pIAeBlueHPV16LR

Plasmid

#185894

Purpose

In vivo gene amplification (HapAmp) of AeBlue + HPV16 L1 expression module at RPL25 locus

Depositor

Claudia Vickers

Article

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)

Expression

Yeast

Mutation

None

Available Since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 VDAC1-Linker-GFP

Plasmid

#211734

Purpose

Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the C-terminus of VDAC1 with a flexible linker.

Depositor

Roland Malli

Article

Pilic et al PLoS One. 2024 Oct 18;19(10):e0311107. d

Insert

Voltage-dependent anion channel 1 (VDAC1 )

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEThT-RvLEAM

Plasmid

#90033

Purpose

To express His-tagged RvLEAM in bacteria

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

RvLEAM

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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