We narrowed to 14,172 results for: cas9
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Plasmid#230861PurposeA truncated version of tracr-L. A 79 nt in the upper stem extension of tracr-L is replaced with 5'-GAAA-3' to generate Δtracr-L.DepositorInsertTruncated version of tracr-L
UseCRISPRExpressionPlantPromoterOsU3Available SinceJuly 15, 2026AvailabilityAcademic Institutions and Nonprofits only -
D10A-Cas9
Plasmid#239383PurposeFor circular RNA-mediated inverse prime editor using nCas9-D10A in HEK294T cellsDepositorInsertnCas9-D10A
UseCRISPRTagsBPNLS and SV40 NLSExpressionMammalianMutationD10A in Cas9PromoterCMVAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Hygro
Plasmid#167899PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EBFP2
Plasmid#167897PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Puro
Plasmid#167903PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable SinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_Blasto
Plasmid#167896PurposePiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmidDepositorInsertdCas9-KRAB-MeCP2
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-Cas9
Plasmid#120352PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonExpressionMammalianPromotertet-on and CBhAvailable SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCF826-recipient_U6-sgRNA-EFS-Cas9-P2A-mCherry2
Plasmid#211688PurposeU6-sgRNA-EFS-Cas9-P2A-mCherry2DepositorInsertSpyCas9 and guide RNA recipient vector
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRE3G-ZIM3-Cas9-P2A-GFP-PGK-Blasti
Plasmid#239604PurposeDoxycycline inducible CRISPRgenee construct with a blasticidin resistanceDepositorAvailable SinceSept. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-CBE6b-IVT
Plasmid#215834PurposeTemplate for in vitro transcription of CBE6b (with SpCas9)DepositorInsertCBE6b-SpCas9-2xUGI
UseIn vitro transcription templateAvailable SinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAct-Fok1:dCas9
Plasmid#62211PurposeExpresses Fok1:dCas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous high specificity genome engineering in Drosophila melanogaster.DepositorInsertFok1-dCas9
ExpressionInsectPromoteract5CAvailable SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
SadCas9 VPR
Plasmid#188514PurposeExpresses FLAG tagged SadCas9 VPRDepositorInsertdCas9
UseCRISPR and LentiviralExpressionMammalianMutationD10A/N580APromoterEF1aAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMA-SpCas9-g7
Plasmid#80790PurposeBsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the arrayDepositorInsertCRISPR gRNA expression cassette (for SpCas9)
UseCRISPRTagsnaExpressionMammalianPromoterU6Available SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMA-SpCas9-g6
Plasmid#80789PurposeBsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the arrayDepositorInsertCRISPR gRNA expression cassette (for SpCas9)
UseCRISPRTagsnaExpressionMammalianPromoterU6Available SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-G6
Plasmid#73437PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.DepositorInsertRepressor G6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceMarch 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX551-CMV-CjCas9
Plasmid#107035PurposeAAV plasmid expressing CjCas9 under control of CMV promoterDepositorInsertCjCas9
UseAAV and CRISPRExpressionMammalianAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC147-pCR8-dCas9VP64
Plasmid#48219PurposedCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP64 activation domain
UseCRISPR and Gateway CloningTagsHA Tag and VP64MutationD10A;H840AAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-APOBEC-nCas9-Ung
Plasmid#198552PurposeExpresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-APOBEC-nCas9-Ung
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
KRAB-dSpCas9
Plasmid#205248PurposeIn vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.DepositorInsertKRAB-dSpCas9
UseCRISPRAvailable SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEC1.2/EC1.1_spCas9_GFP
Plasmid#161933PurposeCRISPR plasmid with spCas9 expressed under egg cell promoter - GFP seed selectionDepositorTypeEmpty backboneUseCRISPRAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only