We narrowed to 9,264 results for: pho

1. pDULE-ABK

   Plasmid

   #49086

   Purpose

   A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments

   Depositor

   Peter Schultz

   Article

   Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.

   Inserts

   tRNA synthetase
   tRNA for a photocrosslinking amino acid
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   L274M, C313A, Y349F

   Promoter

   GlnRS and lpp

   Available since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEM-HE-h_bPAC_cmyc

   Plasmid

   #28134

   Purpose

   optogenetic tool for light modulation of cellular cAMP

   Depositor

   Peter Hegemann

   Article

   Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

   Insert

   humanized photoactivated adenylyl cyclase
   

   Use

   In vitro transcription

   Tags

   Myc

   Expression

   Mutation

   Promoter

   Available since

   June 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. B23(NPM)

   Plasmid

   #34553

   Depositor

   Mary Dasso

   Article

   Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

   Insert

   B23/nucleophosmin (NPM1 Human)
   

   Use

   Tags

   DsRed

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET16b-MYPT1(1-314)

   Plasmid

   #224893

   Purpose

   Bacterial expression of MYPT1(1-314)

   Depositor

   Mathieu Bollen

   Article

   Claes et al Cell Chem Biol. 2023 Dec 21;30(12):1666-

   Insert

   myosin phosphatase targeting subunit 1 (PPP1R12A )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CaMP0.4-FHS-paCaMKII-WPRE3

   Plasmid

   #165429

   Purpose

   Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   FHS-paCaMKII
   

   Use

   AAV

   Tags

   FHS(Flag, HisX6, Strep) tag

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII0.4

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTfR-PATagRFP

   Plasmid

   #31946

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

   Insert

   PATagRFP
   

   Use

   Tags

   TfR

   Expression

   Mammalian

   Mutation

   R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

   Promoter

   CMV

   Available since

   Sept. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD/HisD-PATagRFP

   Plasmid

   #31945

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

   Insert

   PATagRFP
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

   Promoter

   araB

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVSVG-PATagRFP

   Plasmid

   #31947

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

   Insert

   PATagRFP
   

   Use

   Tags

   VSVG

   Expression

   Mammalian

   Mutation

   R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

   Promoter

   CMV

   Available since

   Sept. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-tdTomato-P2A-paCaMKII(K42M)

   Plasmid

   #165433

   Purpose

   Expresses tdTomato and photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   tdTomato-P2A-paCaMKII(K42M)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   K42M: Kinase dead mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PDK1-FLAG-LOV*

    Plasmid

    #202205

    Purpose

    Expresses the photoproximity labeling catalyst LOV* fused to PDK1 in mammalian cells

    Depositor

    Thomas Muir

    Article

    Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

    Insert

    [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (PDK1 Human)
    

    Use

    Tags

    FLAG and LOV*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ensconsin-18-mEos2

    Plasmid

    #99230

    Purpose

    Mammalian expression of the photoconvertible protein mEos2 as an Ensconsin fusion.

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    mEos2
    

    Use

    Tags

    Ensconsin (MAP7)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CRY2-CreN

    Plasmid

    #75268

    Purpose

    The second half of the photo-activatable cre recombinase

    Depositor

    Michael Bruchas

    Article

    Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

    Insert

    second half of the cre recombinase fused to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgRNA3_GAL4UAS-Luciferase reporter

    Plasmid

    #64159

    Purpose

    Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA3 for GAL4UAS-Luciferase reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. RapR-Shp2-mVenus-flag

    Plasmid

    #188655

    Purpose

    Rapamycin regulated Shp2 phosphatase with mVenus and flag tag

    Depositor

    Andrei Karginov

    Article

    Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    Shp2
    

    Use

    Tags

    Flag and mVenus

    Expression

    Mammalian

    Mutation

    iFKBP insertion at V406 with deletion of residues…

    Promoter

    CMV

    Available since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJT11

    Plasmid

    #98308

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

    Insert

    TRPL3ClLIS1>TRPL41B
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. MmSphk2

    Plasmid

    #118598

    Purpose

    Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice

    Depositor

    Kevin Lynch

    Article

    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

    Insert

    Mus musculus Sphingosine Kinase 2 (Sphk2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, 10

    Available since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. UAS::PTPRFb-DN-GFP

    Plasmid

    #37111

    Depositor

    Alvaro Sagasti

    Article

    Wang et al Curr Biol. 2012 Mar 6;22(5):373-82. Epub

    Insert

    UAS::PTPRFb-DN-GFP (ptprfb Zebrafish)
    

    Use

    Tol2 gateway expression vector

    Tags

    EGFP

    Expression

    Mutation

    Deleted aa1386-1909 corresponding to phosphatase …

    Promoter

    10xUAS

    Available since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. Dronpa-Mito-7

    Plasmid

    #57288

    Purpose

    Localization: Mitochondria, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Article

    Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    Dronpa

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pETM41-EcLonA

    Plasmid

    #38330

    Depositor

    Florian Freimoser

    Article

    Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

    Insert

    Lon protease fragment (lon Escherichia coli)
    

    Use

    Tags

    His, MBP, and TEV

    Expression

    Bacterial

    Mutation

    Polyphosphate binding fragment of Lon protease. …

    Promoter

    T7

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

20. sgRNA1_GAL4UAS-Luciferase reporter

    Plasmid

    #64157

    Purpose

    Photoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1 for GAL4UAS-Luciferase reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge