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pLV-U6-gRNA scaffold-UbC-mCherry-PuroR

Plasmid

#232183

Purpose

Lentiviral plasmid for cloning an spCas9 gRNA with puromycin resistance and mCherry

Depositor

Charles Gersbach

Article

Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6, hUbC

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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retro-gRNA-mRFP1

Plasmid

#112914

Purpose

Retrovirus expressing mRFP1 with BBSI cloning sites for sgRNA

Depositor

Christophe Benoist, Diane Mathis

Article

Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

Insert

mRFP1

Use

Retroviral

Promoter

EF1a

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-c/o NL4-3 Env delta CT

Plasmid

#139285

Purpose

HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

codon optimized HIV-1 Env delta cytoplasmic tail

Expression

Mammalian

Mutation

deletion of cytoplasmic tail after residue 710

Promoter

CMV IE

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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myc-KIF5C

Plasmid

#127618

Purpose

N-terminal myc-tagged mouse KIF5C

Depositor

Josef Kittler

Article

Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

Insert

KIF5C (Kif5c Mouse)

Tags

myc

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pLJM1 FRB-myc-RagA

Plasmid

#112740

Purpose

express FRB-myc-RagA

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

FRB-myc-RagA (RRAGA Human)

Use

Lentiviral

Tags

FRB, myc

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLJM1 FRB-myc-RagC

Plasmid

#112741

Purpose

express FRB-myc-RagC

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

FRB-myc-RagC (RRAGC Human)

Use

Lentiviral

Tags

FRB, myc

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pDOXOFF (STOP)99xGly

Plasmid

#211349

Purpose

DOX-regulated lentiviral expression of 99xGly-EGFP-3xHA lacking initiation codon

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

(STOP)99xGly-EGFP-3xHA

Use

Lentiviral

Tags

EGFP-3xHA

Mutation

Start (ATG) codon replaced with stop (TAG) codon

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-Lck-mCherry-ibARK(D110A)

Plasmid

#241242

Purpose

Negative control viral expression vecotor for plasma membrane-tethered ibARK in astrocytes

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

Lck-mCherry-ibARK(D110A)

Use

AAV

Mutation

D110A

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pUTKan-3xHA-GFP-TOM5

Plasmid

#130674

Purpose

Ubiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial membrane of planta

Depositor

Andreas Weber

Article

Kuhnert et al Plant Physiol. 2020 Feb;182(2):692-706.

Insert

Translocase of outer mitochondrial membrane 5 (TOM5 Mustard Weed)

Tags

3xHA and sGFP

Expression

Plant

Promoter

Ubiquitin10

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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RAM-d2TTA-TRE-hM4Di-mCherry-WPREpA

Plasmid

#223670

Purpose

AAV expression of hM4Di from RAM promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

hM4Di

Use

AAV

Tags

mCherry

Promoter

pRAM and TRE

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.dTomato.miR-125b-2

Plasmid

#169302

Purpose

Constitutive overexpression of miR-125b-2 with dTomato as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-125b-2 (MIR125B2 Human)

Use

Lentiviral

Tags

dTomato

Promoter

SFFV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

Plasmid

#138145

Purpose

Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

PspCas13b

Use

Lentiviral

Tags

NLS

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pDF0317 HvsCas7-11 mammalian expression

Plasmid

#172511

Purpose

Encodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

HvsCas7-11 mammalian expression

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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MAC3-FGFR1

Plasmid

#222611

Purpose

Expression of MAC3-tagged FGFR1

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

FGFR1 (FGFR1 Human)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCDH-SIRT2-Flag

Plasmid

#102624

Purpose

Expresses full length SIRT2 isoform 1 in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

SIRT2 (SIRT2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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hTPST2

Plasmid

#11253

Depositor

Hyeryun Choe

Article

Choe et al Cell. 2003 Jul 25. 114(2):161-70.

Insert

TPST2 (TPST2 Human)

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-IRES-Puro GFP-3xFLAG

Plasmid

#201636

Purpose

Over-expression of 3xFLAG-tagged GFP

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

GFP

Use

Lentiviral

Tags

3xFLAG

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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SiC-V2-Cas9G7

Plasmid

#133043

Purpose

Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.

Depositor

Sriram Neelamegham

Article

Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

Insert

Tet repressor

Use

Lentiviral

Tags

Cerulean

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET-30a-METTL17-8XHIS

Plasmid

#215705

Purpose

Express METTL17-8XHIS in C41(DE3) cells

Depositor

Vamsi Mootha

Article

Ast et al Mol Cell. 2024 Jan 18;84(2):359-374.e8.

Insert

METTL17 (METTL17 Human)

Tags

HIS

Expression

Bacterial

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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CRY2PHR(W349R)-mCherry

Plasmid

#75370

Purpose

Expresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CRY2 residues 1-498 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Mutation

W349R

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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