We narrowed to 7,454 results for: ski

1. pHS1477

   Plasmid

   #205970

   Purpose

   MWEE01000013 Cas5-HNH operon (E. coli codon optimized) with His14-tagged Cse2 in pET45b(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas5-HNH all proteins, with His-tagged Cas11
   

   Use

   Tags

   14xHis on Cas11

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)

   Plasmid

   #167268

   Purpose

   Overexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   HOB
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSET-pr-mEos3.1 (mEosFP3.1-A69T)

   Plasmid

   #99221

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEos3.1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A69T for primed conversion applications

   Promoter

   T7

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA pDEST40 Cas9-HMGB1

   Plasmid

   #183195

   Purpose

   Expression clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   HMGB1
   

   Use

   CRISPR

   Tags

   Cas9WT

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO.1P shFXN_2

   Plasmid

   #102971

   Purpose

   Suppression of FXN

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shFXN_2 (FXN Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHS1516

   Plasmid

   #205979

   Purpose

   FLAG-SV40 NLS-Cas7 from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas7 from MWEE01000013, Cas5-HNH
   

   Use

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. CD58-CD4d3+4-bio

   Plasmid

   #73113

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding CD58 with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   CD58 (CD58 Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

8. HA-MBP-TEVpCS-ADGRL4 CTF-FLAG

   Plasmid

   #217709

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRL4 (ADGRL4 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-406

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYY1704

   Plasmid

   #198421

   Purpose

   pBs3:RedNTF:tNOS. A pGreen based plasmid expressing the INTACT reporter RedNTF after the promoter of the pepper Bs3 gene (pBs3). The plasmid has spectinomycin resistance.

   Depositor

   Yuan You

   Article

   You et al Nat Plants. 2023 Jan;9(1):128-141. doi:

   Insert

   RedNTF
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pBs3

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-51b(+)-Caprola_14

    Plasmid

    #194675

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_14 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_14
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-51b(+)-Caprola_15

    Plasmid

    #194676

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_15 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_15
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX Yes-SH2

    Plasmid

    #46532

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Yes (YES1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 152-261

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBS-mHopX

    Plasmid

    #16425

    Depositor

    Jonathan Epstein

    Article

    Chen et al Cell. 2002 Sep 20. 110(6):713-23.

    Insert

    Hopx (hop Mouse)
    

    Use

    In situ template

    Tags

    Expression

    Mutation

    None

    Promoter

    Available since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX Txk-SH2

    Plasmid

    #46526

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Txk (Txk Mouse)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 138-258

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDule-IBBN (G2)

    Plasmid

    #85500

    Purpose

    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

    Insert

    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32G L65E F108W Q109M D158S R162K

    Promoter

    lpp (constitutive)

    Available since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-MBP-TEVpCS-ADGRB2 CTF-FLAG

    Plasmid

    #217682

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRB2 (BAI2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-911

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHS1068

    Plasmid

    #205962

    Purpose

    NZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promoters

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    DinG HNH proteins and CRISPR array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac (proteins), pJ23119 (crRNA)

    Available since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pInducer10-UBC9-sh4

    Plasmid

    #168988

    Purpose

    Expresses RFP cDNA and UBC9 shRNA 4 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Ubiquitin Conjugating Enzyme 9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubc promoter

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMM9-MAPKK2-KW71 [delta(48-55)/S222D/S226D]

    Plasmid

    #40806

    Depositor

    Natalie Ahn

    Article

    Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

    Insert

    MAP2K2 (MAP2K2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta(48-55) + S222D + S226D

    Promoter

    MSV-LTR

    Available since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pGEX Sh3bp2-SH2

    Plasmid

    #46484

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Sh3bp2 (SH3BP2 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 450-561

    Promoter

    Tac

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge