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MTK3a_013

Plasmid

#123765

Purpose

Encodes msGFP as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

msfGFP

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK2_015

Plasmid

#123707

Purpose

Encodes pSynTF (miniCMV core) as a Type 2 part to be used in the MTK sytem

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSynTF

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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cmv 16 E7 C91S

Plasmid

#13689

Depositor

Karl Munger

Article

Gonzalez et al J Virol. 2001 Aug . 75(16):7583-91.

Insert

HPV 16 E7 C91S (E7 Human)

Expression

Mammalian

Mutation

aa 91 from C to S

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
MXS_H2B

Plasmid

#62432

Purpose

MXS_chaining vector with H2B

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Histone 2B with leading Kozak sequence

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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p185 pK7-HDGF (GFP)

Plasmid

#11058

Depositor

Ramesh Shivdasani

Article

Tou et al Mol Cell Biol. 2004 Apr . 24(8):3132-9.

Insert

HDGF (Hdgf Rat)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
MTK3_004

Plasmid

#123720

Purpose

Encodes hph (hygromycin B phosphotransferase) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Hygro Resistance

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3_005

Plasmid

#123721

Purpose

Encodes ble (Zeocin resistance) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Zeocin Resistance

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-08

Plasmid

#119987

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mClover3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mClover3

Use

Pcr template

Tags

mClover3

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pPD546 ZF42x6-Compact_EYFP

Plasmid

#138860

Purpose

EYFP reporter vector for the ZF42x6-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF42x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF42x6-Compact

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPD530 VP16-ZF42

Plasmid

#138760

Purpose

Constitutive expression of VP16-ZF42 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF42

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD542 ZF14x6-Compact_EYFP

Plasmid

#138856

Purpose

EYFP reporter vector for the ZF14x6-Compact promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF14x6-Compact

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF14x6-Compact

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPD531 VP16-ZF54

Plasmid

#138762

Purpose

Constitutive expression of VP16-ZF54 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF54

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pPD527 VP16-ZF14

Plasmid

#138756

Purpose

Constitutive expression of VP16-ZF14 under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VP16-ZF14

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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MXS_SV40pA

Plasmid

#62426

Purpose

MXS_chaining vector with SV40pA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

SV40 poly A

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MTK2_026

Plasmid

#123718

Purpose

Encodes crippled pSFFV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pSFFVc

Expression

Mammalian

Mutation

Crippled Kozac sequence

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBW810_pCAG-PV7-loxN-F14-mCherry-BGHpA

Plasmid

#87586

Purpose

PV7 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mCherry

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pMaCTag-14

Plasmid

#119993

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeptune2.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mNeptune2.5

Use

Pcr template

Tags

mNeptune2.5

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch13_102010574-gRNA-for

Plasmid

#81218

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch13 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pMAD-Gate3

Plasmid

#25942

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

Insert

Tags

VP16 Fusion Protein

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pMBD-Gate3

Plasmid

#25959

Depositor

Kamil Onder

Article

Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

Insert

Tags

GAL4 Fusion Protein

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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MXS_CMVenhancer

Plasmid

#62419

Purpose

MXS_chaining vector with CMVenhancer

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

CMV enhancer

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_MCS2

Plasmid

#62396

Purpose

MXS_chaining vector with MCS2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

EcoRV-MluI-XhoI-EcoRI-BglII-KpnI-SalI MCS

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAG144 SLC23A2 3'UTR

Plasmid

#12002

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N9 3'UTR (SLC23A2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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MTK1_018

Plasmid

#123681

Purpose

Encodes ConL8 as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L8

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBSKΔB-CBh-Cas9n-pA

Plasmid

#82617

Purpose

Expresses Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

SpCas9n

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3b_029

Plasmid

#123807

Purpose

Encodes Tet-On 3G transactivator protein as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TET3G

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MXS_EF1a

Plasmid

#62421

Purpose

MXS_chaining vector with EF1a

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

EF1a Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAG199 QKI 3'UTR

Plasmid

#12011

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

QKI 3'UTR (QKI Human)

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pMaCTag-24

Plasmid

#120003

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with TAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TAP

Use

Pcr template

Tags

TAP

Promoter

None

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-10

Plasmid

#119989

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mKOk

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKOk

Use

Pcr template

Tags

mKOk

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge