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We narrowed to 35,392 results for: CaS;

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Showing: 10051 - 10100 of 35392 results

  1. pXZ13lacZalphaKanR

    Plasmid
    #160230
    Purpose
    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.
    Depositor
    Ian Duncan
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Oct. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    160230-plasmid-map-sequence-id-314765

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  2. pEGFP-rUSP19CYT-CAmut

    Plasmid
    #155250
    Purpose
    for mammalian overexpression of the mutated CYT isoform of rat USP19 fused to GFP at N-terminus
    Depositor
    Simon Wing
    Insert
    USP19 (Cytoplasmic isoform) CA mutant (Usp19 Rat)
    Expression
    Mammalian
    Mutation
    Cys(545) to Ala
    Promoter
    CMV
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155250-plasmid-map-sequence-id-311949

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  3. pEGFP-rUSP19ER-CAmut

    Plasmid
    #155249
    Purpose
    for mammalian overexpression of the mutated ER isoform of rat USP19 fused to GFP at N-terminus
    Depositor
    Simon Wing
    Insert
    USP19 (ER isoform) CA mutant (Usp19 Rat)
    Expression
    Mammalian
    Mutation
    Cys(545) to Ala
    Promoter
    CMV
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    155249-plasmid-map-sequence-id-311947

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  4. pDD376

    Plasmid
    #137022
    Purpose
    SapTrap mKate2-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized mKate2
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized mKate2
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137022-plasmid-map-sequence-id-274081

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  5. pRARB.1.0-gDNA

    Plasmid
    #132471
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    RARB (RARB Human)
    Use
    CRISPR
    Available Since
    Dec. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    132471-plasmid-map-sequence-id-267178

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  6. UC16m

    Plasmid
    #121038
    Purpose
    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    gQi Cas9 gRNA UC part
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121038-plasmid-map-sequence-id-236383

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  7. UC17m

    Plasmid
    #121039
    Purpose
    MoClo golden gate assembly DE part for gV1 gRNA (guide RNA for S. pyogenes Cas9; sequence from doi: [10.15252/msb.20145735]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    gV1 Cas9 gRNA UC part
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121039-plasmid-map-sequence-id-236352

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  8. UCT1m

    Plasmid
    #121041
    Purpose
    MoClo golden gate assembly DE part for gQi gRNA (guide RNA for S. pyogenes Cas9; sequence from DOI: 10.1016/j.cell.2013.02.022). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    UCT-part guide RNA (Stanley Qi sequence)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121041-plasmid-map-sequence-id-245418

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  9. C114m

    Plasmid
    #121013
    Purpose
    MoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9, with bbsI cut sites removed synonymously). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9 with no bbsI or BsaI sites
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121013-plasmid-map-sequence-id-245424

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  10. pSB700-TTN-Puro

    Plasmid
    #128323
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (human cells)
    Depositor
    Alejandro Chavez
    Insert
    gRNA against human TTN for activation
    Expression
    Mammalian
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128323-plasmid-map-sequence-id-255024

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  11. pICSL4723_ Pi21

    Plasmid
    #126892
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126892-plasmid-map-sequence-id-249677

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  12. pICSL4723_ CUL3

    Plasmid
    #126891
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126891-plasmid-map-sequence-id-250181

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  13. pICSL4723_ PLDbeta1

    Plasmid
    #126890
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126890-plasmid-map-sequence-id-250163

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  14. pICSL4723_ WRKY28_1

    Plasmid
    #126887
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126887-plasmid-map-sequence-id-249685

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  15. pICSL4723_ Rac5

    Plasmid
    #126885
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126885-plasmid-map-sequence-id-249710

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  16. pICSL4723_ Rac4

    Plasmid
    #126884
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126884-plasmid-map-sequence-id-249713

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  17. pENTRY4_ Dja6_2

    Plasmid
    #126895
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126895-plasmid-map-sequence-id-250173

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  18. pENTRY4_ Rac4

    Plasmid
    #126896
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126896-plasmid-map-sequence-id-250171

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  19. pENTRY4_ CUL3

    Plasmid
    #126903
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126903-plasmid-map-sequence-id-250107

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  20. pENTRY4_ WRKY28_1

    Plasmid
    #126899
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    126899-plasmid-map-sequence-id-250041

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  21. pENTRY4_ WRKY28_2

    Plasmid
    #126900
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    126900-plasmid-map-sequence-id-249965

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  22. pENTRY4_ PLDbeta1

    Plasmid
    #126902
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126902-plasmid-map-sequence-id-249806

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  23. pENTRY4_ Dja6_1

    Plasmid
    #126894
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126894-plasmid-map-sequence-id-249699

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  26. MLM3636

    Plasmid
    #43860
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter
    Depositor
    Keith Joung
    Insert
    None
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    43860-plasmid-map-sequence-id-194368

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  27. pDD362

    Plasmid
    #137023
    Purpose
    SapTrap HaloTag-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized Halo Tag
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized Halo Tag
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137023-plasmid-map-sequence-id-274041

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  28. plenti guide Thy1.1

    Plasmid
    #128063
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128063-plasmid-map-sequence-id-255154

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  29. LentiCROP-PuroR-P2A-GFP-U6

    Plasmid
    #200029
    Purpose
    Lentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAs
    Depositor
    Jay Shendure
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    PuroR-P2A-GFP
    Expression
    Mammalian
    Available Since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    200029-plasmid-map-sequence-id-393200

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  30. pCMV-ABE8eWQ

    Plasmid
    #161815
    Purpose
    Expresses ABE8eWQ in mammalian cells
    Depositor
    Sangsu Bae
    Insert
    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available Since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    161815-plasmid-map-sequence-id-320654

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  31. pXR003_puroR

    Plasmid
    #219819
    Purpose
    hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    219819-plasmid-map-sequence-id-440390

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  32. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid
    #226429
    Purpose
    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.
    Depositor
    Alon Goren
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226429-plasmid-map-sequence-id-458099

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  33. pET-MBP-NLS-Geo_st

    Plasmid
    #87703
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS
    Depositor
    Jennifer Doudna
    Insert
    GeoCas9
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87703-plasmid-map-sequence-id-176992

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  35. pZDonor rosa26 LSL-mMALAT1

    Plasmid
    #140691
    Purpose
    generate mouse MALAT1 conditional transgenic mice using CRISPR system targeting mouse Rosa 26 region
    Depositor
    Jianjun Zhao
    Insert
    mouse MALAT1 (Malat1 Mouse)
    Use
    Mouse Targeting
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140691-plasmid-map-sequence-id-299282

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  36. pZFX-donor

    Plasmid
    #112366
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZFX with GFP
    Depositor
    Kevin White
    Insert
    ZFX homology arms flanking EGFP-IRES-Neo cassette (ZFX Human)
    Use
    CRISPR
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112366-plasmid-map-sequence-id-229714

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  37. pAAV-dSa-VPR

    Plasmid
    #99651
    Purpose
    Expresses tripartite VP64-p65-RTA activator fused to C term. of dead Sa Cas9
    Depositor
    George Church
    Insert
    dCas9
    Use
    AAV
    Tags
    VP64-p65-RTA activator
    Mutation
    dead Cas9
    Available Since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    99651-plasmid-map-sequence-id-191797

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  38. pBHM2641

    Plasmid
    #229525
    Purpose
    Carries OsTIR1-t2a-mNG-AID*, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Use
    CRISPR
    Tags
    t2a-mNeonGreen-AID*
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Promoter
    TEF1
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229525-plasmid-map-sequence-id-459550

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  39. pcU6.3_gRNA_c1

    Plasmid
    #221381
    Purpose
    Chicken-specific U6 sgRNA expression mini-vector with 10x capture sequence 1
    Depositor
    James Briscoe, Tatjana Sauka-Spengler
    Type
    Empty backbone
    Use
    CRISPR
    Promoter
    G. gallus U6.3
    Available Since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221381-plasmid-map-sequence-id-454411

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  40. pDD288

    Plasmid
    #137017
    Purpose
    mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    mNG-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized mNG
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137017-plasmid-map-sequence-id-274039

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  41. pB-CAG-PE2-NatMX

    Plasmid
    #158567
    Purpose
    Piggybac vector that allows for the generation of PE2-expressing stable cell lines
    Depositor
    Alejandro Chavez
    Insert
    PE2
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    158567-plasmid-map-sequence-id-309882

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  42. bU6-sgSik1_1st-mU6-sgSik2-hU6-sgSik3_1st

    Plasmid
    #177232
    Purpose
    Expresses Sik1(bU6), Sik2 (mU6) and Sik3 (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_1st/sgSik2/sgSik3_1st
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    177232-plasmid-map-sequence-id-363978

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  43. AAVS1_Puro_H2BK_mNG_hSLBP(18-108)

    Plasmid
    #191529
    Purpose
    Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    H2BK_mNG_hSLBP(18-108)
    Use
    CRISPR and Synthetic Biology
    Tags
    mNeonGreen (mNG)
    Expression
    Mammalian
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191529-plasmid-map-sequence-id-378106

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  44. bU6-sgMark2-mU6-sgMark3-hU6-sgMark4

    Plasmid
    #177235
    Purpose
    Expresses Mark2 (bU6), Mark3 (mU6) and Mark4 (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgMark2/sgMark3/sgMark4
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    177235-plasmid-map-sequence-id-352696

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  45. bU6-sgNeo1-mU6-sgNT-hU6-sgNeo2

    Plasmid
    #177231
    Purpose
    Expresses neomycin gRNA's ( bU6 and hU6 ), non-targeting gRNA ( mU6 ) and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgNT1/sgNeo2
    Use
    Lentiviral
    Promoter
    bU6/mU6/hU6
    Available Since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    177231-plasmid-map-sequence-id-352660

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  47. ipUSEPR-sg-Hs-PCNA

    Plasmid
    #188686
    Purpose
    control sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPCNA (PCNA Human)
    Use
    CRISPR and Lentiviral
    Mutation
    WT
    Available Since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188686-plasmid-map-sequence-id-375116

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  48. pPITX2-donor

    Plasmid
    #176372
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    PITX2 (PITX2 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176372-plasmid-map-sequence-id-347400

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  49. pDD291

    Plasmid
    #137019
    Purpose
    mKate2-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    mKate2-GLO^SEC^3xFlag + ccdB
    Use
    CRISPR and Cre/Lox
    Tags
    3xFlag and C. elegans GLO-optimized mKate2
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137019-plasmid-map-sequence-id-274037

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  50. TC9nickpAB

    Plasmid
    #71311
    Purpose
    used as a template for in vitro transcription of Cas9 nickase mRNA after linearizing with BbsI
    Depositor
    Karl Degenhardt
    Insert
    Cas9 nickase
    Use
    In vitro transcription
    Tags
    NLS
    Mutation
    D10A nickase
    Promoter
    T7
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    71311-plasmid-map-sequence-id-131863

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Showing: 10051 - 10100 of 35392 results