We narrowed to 8,036 results for: Dos;

1. mcherry-Myo 9b R1695M

   Plasmid

   #134958

   Purpose

   Expression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.

   Depositor

   Martin Bähler

   Article

   Hemkemeyer et al J Biol Chem. 2021 Jan-Jun;296:100136. do

   Insert

   Myo 9b (rat) with 3' UTR. GAP minus mutant R1695M (Myo9b Rat)
   

   Use

   Tags

   mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRC/CMV-TYK2-VSV

   Plasmid

   #139344

   Purpose

   Expression of TYK2 protein

   Depositor

   Sandra Pellegrini

   Article

   Li et al PLoS One. 2020 Jan 21;15(1):e0225289. do

   Insert

   TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
   

   Use

   Tags

   VSV

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO-Intron-CterFlag-MePCE

   Plasmid

   #113549

   Purpose

   Mammalian expression plasmid that can be used with the Flp-In T-REx system.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   MePCE (MEPCE Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3_ERBB2-V5

   Plasmid

   #214612

   Purpose

   ERBB2 receptor for ERBB2/ERBB3 heterodimer split TEV recuitment assay

   Depositor

   Michael Wehr

   Article

   Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Flag-Tet3 CD Mut

   Plasmid

   #72222

   Purpose

   Express flag-tagged mouse Tet3 catalytic domain - catalytic dead mutant.

   Depositor

   Yi Zhang

   Article

   Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

   Insert

   Ten-Eleven Translocation 3
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   The plasmid expresses TET3 catalytic domain (cata…

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pZDonor-Nm-Cas9-gRNA-hU6

   Plasmid

   #61366

   Purpose

   empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-FLEX-VAMP2:HRP

   Plasmid

   #60659

   Purpose

   Labels synaptic vesicles for electron microscopy

   Depositor

   Scott Sternson

   Article

   Atasoy et al Nat Neurosci. 2014 Dec;17(12):1830-9. do

   Insert

   VAMP2:HRP
   

   Use

   AAV and Cre/Lox

   Tags

   Myc

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCAG-N-CretrcintG

   Plasmid

   #69572

   Purpose

   One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

   Insert

   kozak consensus - N-CretrcintG
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU3-gRNA

   Plasmid

   #53063

   Purpose

   Transcription of guide RNA (gRNA) in rice cells

   Depositor

   Caixia Gao

   Article

   Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

   Insert

   OsU3 promoter + gRNA scaffold
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   rice U3

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-C-CreintG

    Plasmid

    #69573

    Purpose

    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do

    Insert

    kozak consensus - C-CreintG
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-TetOn-Cdt1-mCherry

    Plasmid

    #193760

    Purpose

    Doxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    Cdt1-mCherry (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mutation

    WT

    Promoter

    TRE

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-AcrIIA17_Efa-NLS(SV40) (KAC91)

    Plasmid

    #134333

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Efa with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA17_Efa
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. plenti hSyn PGK1-HALO

    Plasmid

    #220910

    Purpose

    Neuronal specific expression of the fusion construct PGK1-HALO

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    PGK1-HALO (PGK1 Human)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC841 - pAAV minP secNLuc

    Plasmid

    #82498

    Purpose

    Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

    Depositor

    Brandon Harvey

    Article

    Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

    Insert

    secNLuc
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    c-Fos minimal promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103_PDCD6_EF-hand

    Plasmid

    #110009

    Purpose

    Protein expression and purification of PDCD6_EF-hand

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PDCD6_EF-hand (PDCD6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. tdKatushka2-VASP-5

    Plasmid

    #56049

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 586, Emission: 632

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Insert

    VASP
    

    Use

    Tags

    tdKatushka2

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. PXPR007 sgGFP-1

    Plasmid

    #74960

    Purpose

    Cas9 + sgGFP-1 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. mVenusN-Cavin3

    Plasmid

    #108889

    Purpose

    Expression of Cavin3 fused to the N-terminus of mVenus for bimolecular fluorescence complementation

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    Cavin3 (altered codon usage) (CAVIN3 Synthetic, Human)
    

    Use

    Tags

    mVenus1-155

    Expression

    Mammalian

    Mutation

    altered codon usage

    Promoter

    Available since

    March 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)

    Plasmid

    #78509

    Purpose

    Construct for expressing CD45R0 (extracellular domain) His10 in Sf9

    Depositor

    Ron Vale

    Article

    Su et al Science. 2016 Apr 29;352(6285):595-9. do

    Insert

    CD45R0Ex (PTPRC Human)
    

    Use

    Tags

    His10 and SNAPf

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-EGFP-C1-HsDCP2-E148Q_U

    Plasmid

    #147650

    Purpose

    Mammalian Expression of HsDCP2-E148Q

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsDCP2-E148Q (DCP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    two silent mutations compared to the sequence giv…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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