We narrowed to 18,458 results for: Met

1. plentiCRISPR-v2 Puro SPNS1_sg2

   Plasmid

   #218531

   Purpose

   sgRNA targeting human SPNS1

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   SPNS1 (SPNS1 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. plentiCRISPR-v2 Puro SPNS1_sg1

   Plasmid

   #218530

   Purpose

   sgRNA targeting human SPNS1

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   SPNS1 (SPNS1 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKG-GW1-SRT1

   Plasmid

   #203171

   Purpose

   Yeast expression vector for yeast SRT1

   Depositor

   William Sessa

   Article

   Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

   Insert

   SRT1 (SRT1 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   TDH3

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. 2x3APWWP_K299E_D333A-IFP2.0C

   Plasmid

   #215753

   Purpose

   Expresses binding-deficient K299E/D333A mutant of 2xDNMT3A PWWP domain fused to C-terminal part of IFP2.0 as negative control for H3K36me2/3 detection in BiAD sensors

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   2x3APWWP_K299E_D333A-IFP2.0C
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCF702

   Plasmid

   #209650

   Purpose

   Suicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicus

   Depositor

   Bastian Molitor

   Article

   Fink et al Appl Environ Microbiol. 2023 Jul 26;89(7

   Inserts

   Downstream homologous region
   thermostable neomycin phosphotransferase gene
   Upstream homologous region
   

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TLNRD1_35-345_eGFP

   Plasmid

   #208881

   Purpose

   Express eGFP-TLNRD1 lacking N and Cterm domains (TLNRD1 amino acids 35-345) in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   TLNRD1 (TLNRD1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   amino acid sequence 35-345

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. mito-PDCD10-mScarlet-I

   Plasmid

   #208887

   Purpose

   Express mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   CCM3 (PDCD10 Human)
   

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   PDCD10 targeted to the mitonchondria

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TLNRD1_5H_eGFP

   Plasmid

   #208861

   Purpose

   Express eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   TLNRD1 5 helix bundle (TLNRD1 Human)
   

   Expression

   Mammalian

   Mutation

   Deleted amino acid 142-273

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA

   Plasmid

   #174897

   Purpose

   ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   sf GFP
   

   Expression

   Mammalian

   Mutation

   150TAA in sfGFP

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA

    Plasmid

    #174898

    Purpose

    opal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TGA in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKG-GW1-RER2

    Plasmid

    #203170

    Purpose

    Yeast expression vector for yeast RER2

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    RER2 (RER2 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKG-GW1-GlcisPT

    Plasmid

    #203172

    Purpose

    Yeast expression vector for Giardia cis-prenyltransferase

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Insert

    UPPS
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-RAPH1Δ536-587

    Plasmid

    #194865

    Purpose

    Express eGFP-RAPH1 lacking amino acids 536-587 in mammalian cells. This constructs does not localise to filopodia anymore.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    RAPH1 with amino acids 536-587 deleted

    Promoter

    CMV

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSFDuet-1_VNp-mNeongreen-Uricase

    Plasmid

    #182417

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen-uricase fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-uricase
    

    Tags

    Vesicle Nucleating peptide (VNp) & mNeongreen

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSFDuet-1_mNeongreen-StefinA

    Plasmid

    #182418

    Purpose

    Bacterial expression of mNeongreen-stefinA fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    mNeongreen-stefin (CSTA Human)
    

    Tags

    mNeongreen

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNHT7::UMSBP::sNluc2_ORF1

    Plasmid

    #186765

    Purpose

    An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Promoter

    T7

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNHT7::ENO::sNluc2

    Plasmid

    #186770

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) with enolase 5' and 3' UTRs

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNHT7::RPL15::sNluc0

    Plasmid

    #186771

    Purpose

    An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0) with RPL15 5' and 3' UTRs

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNHT7::RPL15::hNluc

    Plasmid

    #186772

    Purpose

    An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with RPL15 5' and 3' UTRs

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNHT7::YB1::hNluc

    Plasmid

    #186774

    Purpose

    An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with y-box binding protein 5' and 3' UTRs

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    Nanoluciferase
    

    Promoter

    T7

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge