We narrowed to 9,777 results for: 158

1. pJK_ToeRepG2_N02_switch

   Plasmid

   #132742

   Purpose

   T7 RNAP-driven expression of second-generation toehold repressor index 2 switch RNA with a GFP-ASV reporter

   Depositor

   Alexander Green, Peng Yin

   Article

   Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

   Insert

   ToeRepG2_N02_switch
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pXPR_003 sgMSLN

   Plasmid

   #193588

   Purpose

   MSLN knockout

   Depositor

   William Hahn

   Article

   Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

   Insert

   sgMSLN (MSLN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA5/FRT-ChemoL-ATP

   Plasmid

   #193831

   Purpose

   CMV overexpression of ChemoL-ATP in mammalian cell lines

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoL-ATP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   EGFP:A206K; HT7: L271E

   Promoter

   CMV

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p2T-CMV-evoCjCas9-eAID-BlastR

   Plasmid

   #194062

   Purpose

   Mammalian expression of evoCjCas9 eAID

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-eAID
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSCL.028

   Plasmid

   #184965

   Purpose

   Test effect of extending -Eco1 a1/a2 in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: RT and ncRNA(extended), a1/a2 length: 27
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   human codon optimized RT, a1/a2 length extended t…

   Promoter

   Gal7

   Available since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p426 46Q GPD

   Plasmid

   #1182

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 46Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pCMV-BE4-AmAPOBEC1

   Plasmid

   #138342

   Purpose

   Mammalian expression plasmid for BE4-AmABOBEC1

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-AmAPOBEC1
   

   Use

   Synthetic Biology

   Tags

   BP-NLS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CMV-AsCas12f-v4.1

   Plasmid

   #204636

   Purpose

   Expression of AsCas12f v4.1 in mammalian cells

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

   Insert

   AsCas12f-v4.1
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   D169K, N199K, N328G, D364R

   Promoter

   CMV

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a_Pro-IL-36α

   Plasmid

   #214343

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL36A (IL36A Human)
   

   Use

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKH159_FLAG-APEX2-YIPF4 WT

    Plasmid

    #214978

    Purpose

    Flag-APEX2 tagged YIPF4

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    FLAG-APEX2-YIPF4 (YIPF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV- CAG-h2BmRuby2FLAG

    Plasmid

    #116872

    Purpose

    Nuclear localized mRuby2FLAG

    Depositor

    Loren Looger

    Article

    Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

    Insert

    H2BmRuby2FLAG
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-LbCpf1-U6_FlipArray

    Plasmid

    #109349

    Purpose

    Lentiviral vector to express Cpf1 from EFS promoter, as well as a Cpf1 FlipArray from U6 promoter.

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Commun. 2018 May 15;9(1):1903. doi:

    Insert

    U6-FlipArray
    

    Use

    CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p426 25Q GPD

    Plasmid

    #1181

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 25Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

14. p426 103Q GAL

    Plasmid

    #1188

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 103Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pPB-R1R2_EF1aVP64dCas9p300_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113344

    Purpose

    Expresses VP64-dCas9-p300 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    VP64-dCas9-p300core-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-LbCas12a

    Plasmid

    #213743

    Purpose

    For circular RNA-mediated prime editor using LbCas12a in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    LbCas12a
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBWB424

    Plasmid

    #140638

    Purpose

    RSF1010-Cas9-vanAB gRNA. Vector with constitutive Cas9 and gRNA targeting vanAB locus.

    Depositor

    Keith Tyo

    Article

    Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPB-R1R2_EF1adCas9p300_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113342

    Purpose

    Expresses dCas9-p300 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    dCas9-p300core-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYZ_3WJrep_N19_switch

    Plasmid

    #132723

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 19 switch RNA with a GFP-ASV reporter

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N19_switch
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-BE4-RrA3F

    Plasmid

    #138340

    Purpose

    Mammalian expression plasmid for BE4-RrA3F

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-RrA3F
    

    Use

    Tags

    BP-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p426 25Q GAL

    Plasmid

    #1187

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 25Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

22. FRB-polySH33-EGFP

    Plasmid

    #175792

    Purpose

    Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    NCK1 (NCK1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-51b(+) ChemoG 3.2

    Plasmid

    #193803

    Purpose

    Protein production of ChemoG3.2 in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG 3.2
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    EGFP:A206K, T225R; HT7: L271E

    Promoter

    T7

    Available since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p426 72Q GPD

    Plasmid

    #1183

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 72Q (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

25. pMSCV-Myc-Caspase-4_W267N

    Plasmid

    #214311

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    W267N

    Promoter

    MESV

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. nGR2175-gs10-NLS-VPR

    Plasmid

    #188264

    Purpose

    Plasmid ensures constitutive expression of the N-terminal fragment of split GR2 (aa 1-175), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    nGR2175
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FKBP-CD28TMD-NTEVp(WT)

    Plasmid

    #137826

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(WT)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAR3040-spitz

    Plasmid

    #38341

    Depositor

    Norbert Perrimon

    Article

    Rutledge et al Genes Dev. 1992 Aug;6(8):1503-17.

    Insert

    spitz (spi Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

29. pLX311 LacZ

    Plasmid

    #117731

    Purpose

    Open reading frame vector encoding LacZ

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    LacZ
    

    Use

    Lentiviral

    Tags

    2X V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJK_ToeRepG2_N02_trigger

    Plasmid

    #132745

    Purpose

    T7 RNAP-driven expression of second-generation toehold repressor index 2 trigger RNA

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    ToeRepG2_N02_trigger
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge