We narrowed to 39,099 results for: Eras

1. EV-D68 2A protease; 6xHis-MBP-TEV tag, low expression levels

   Plasmid

   #204803

   Purpose

   Bacterial expresison of Enterovirus D68 2A protease

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   D68 2A protease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving

   Plasmid

   #204811

   Purpose

   Bacterial expresison of Enterovirus A71 2A protease

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   A71 2A protease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. iF60 PB-LoxStopLox-Zim3-dCas9-mScarlet-puro-BFP

   Plasmid

   #204729

   Purpose

   Cre-inducible CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. iF57 PB-FrtStopFrt-Zim3-dCas9-mScarlet-puro-BFP

   Plasmid

   #204727

   Purpose

   Frt-inducible CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. iE65 PB-Zim3-XTEN-dCas9-mApple-puro-BFP

   Plasmid

   #204724

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pED1a6

   Plasmid

   #134787

   Purpose

   Luciferase reporter for quadruplet codon TAGA

   Depositor

   Ahmed Badran

   Article

   DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

   Insert

   luxAB-357-TAGA
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   357-TAGA

   Promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pED1a20

   Plasmid

   #134788

   Purpose

   Luciferase reporter for quadruplet codon CAGG

   Depositor

   Ahmed Badran

   Article

   DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

   Insert

   luxAB-357-CAGG
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   357-CAGG

   Promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pED1a34

   Plasmid

   #134789

   Purpose

   Luciferase reporter for quadruplet codon AGGG

   Depositor

   Ahmed Badran

   Article

   DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

   Insert

   luxAB-357-AGGG
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   357-AGGG

   Promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pED1a36

   Plasmid

   #134790

   Purpose

   Luciferase reporter for quadruplet codon CGGT

   Depositor

   Ahmed Badran

   Article

   DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

   Insert

   luxAB-357-CGGT
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   357-CGGT

   Promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pED1a39

    Plasmid

    #134791

    Purpose

    Luciferase reporter for quadruplet codon TACA

    Depositor

    Ahmed Badran

    Article

    DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

    Insert

    luxAB-357-TACA
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    357-TACA

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pED1a71

    Plasmid

    #134792

    Purpose

    Luciferase reporter for quadruplet codon AGGA

    Depositor

    Ahmed Badran

    Article

    DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

    Insert

    luxAB-357-AGGA
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    357-AGGA

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pED1a89

    Plasmid

    #134793

    Purpose

    Luciferase reporter for quadruplet codon CGGC

    Depositor

    Ahmed Badran

    Article

    DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

    Insert

    luxAB-357-CGGC
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    357-CGGC

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAcrVIA4

    Plasmid

    #164863

    Purpose

    Inducible expression of AcrVIA4 in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    AcrVIA4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAcrVIA7

    Plasmid

    #164864

    Purpose

    Inducible expression of AcrVIA7 in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

    Insert

    AcrVIA7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGAT

    Plasmid

    #112587

    Purpose

    Used for expression of protein in E. coli as Thrombin cleavable 6His-tag-GST fusions with a blunt end restriction site immediately after thrombin recognition sequence

    Depositor

    Marko Hyvönen, Johan Peränen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Use

    Tags

    6His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTS36.6

    Plasmid

    #106264

    Purpose

    Expression of luc, ekivR, esyn1, mutated P2A

    Depositor

    Vera Meyer

    Article

    Schuetze et al Microb Cell Fact. 2017 Sep 25;16(1):162.

    Insert

    luc-P2A*-ekivR-P2A*-esyn1
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mutation

    P2A: changed final proline to alanine

    Promoter

    Tet-On

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PP318

    Plasmid

    #78941

    Purpose

    Expresses GFP. Figure 3. Strain 5.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJOG292

    Plasmid

    #80586

    Purpose

    empty vector (lacZ) for 2nd generation recipient assembly

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJOG294

    Plasmid

    #80587

    Purpose

    dummy module (ccdB) for 2nd generation recipient assembly

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    ccdB
    

    Use

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PP297

    Plasmid

    #78937

    Purpose

    Expresses GFP. Figure 3. Strain 3.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. PP298

    Plasmid

    #78938

    Purpose

    Expresses GFP. Figure 3. Strain 4.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PP299

    Plasmid

    #78939

    Purpose

    Expresses GFP. Figure 3. Strain 6.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PP300

    Plasmid

    #78940

    Purpose

    Expresses GFP. Figure 3. Strain 7.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PP296

    Plasmid

    #78936

    Purpose

    Expresses GFP. Figure 3. Strain 2.

    Depositor

    Timothy Lu

    Article

    Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. -833pSia-Luc

    Plasmid

    #32989

    Depositor

    Sergei Sokol

    Article

    Fan et al Proc Natl Acad Sci U S A. 1998 May 12;95

    Insert

    Siamois promoter (sia1.L Frog)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    Siamois

    Available since

    Jan. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

26. pMDLg/pRRE

    Plasmid

    #12251

    Purpose

    3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)

    Depositor

    Didier Trono

    Article

    Dull et al J Virol. 1998 Nov . 72(11):8463-71.

    Insert

    HIV-1 GAG/POL
    

    Use

    Lentiviral; Packaging

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p6XHis-T7(P266L)

    Plasmid

    #174866

    Purpose

    Expresses a T7 RNA Polymerase that produces long RNA transcripts in high yield and generates fewer abortive transcripts.

    Depositor

    Anna Pyle

    Article

    Chillon et al Methods Enzymol. 2015;558:3-37. doi: 10.

    Insert

    T7 RNA polymerase
    

    Use

    Tags

    6XHis-tag

    Expression

    Bacterial

    Mutation

    P266L

    Promoter

    lac promoter

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PPRE X3-TK-luc

    Plasmid

    #1015

    Purpose

    PPAR reporter. 3xDR1 sites upstream of a luciferase reporter.

    Depositor

    Bruce Spiegelman

    Article

    Kim et al Proc Natl Acad Sci U S A 1998 Apr 14;95(

    Insert

    PPRE
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. iE64 PB-N-term_mycNLS-Zim3-XTEN-dCas9-mScarlet-puro-BFP

    Plasmid

    #204723

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PB-TO-MYOD1-shOct4

    Plasmid

    #182309

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    MYOD1-shOct4 (MYOD1 Human)
    puro-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    shOct4

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge