We narrowed to 39,988 results for: ANT

1. pmTurquoise2-T2A-Venus(L68V)

   Plasmid

   #60494

   Purpose

   Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   mTurquoise2-T2A-mVenus(L68V)
   

   Tags

   T2A peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3-EF1aH c-Met

   Plasmid

   #86498

   Purpose

   Mammalian expression of human c-Met

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   Met (MET Human)
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5-HA-SLC38A9.1

   Plasmid

   #71869

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Jan 9;347(6218):188-94. do

   Insert

   SLC38A9.1 (SLC38A9 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

4. plc242-GFP-WDR24

   Plasmid

   #87050

   Purpose

   mammalian expression of WDR24

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   WDR24 (WDR24 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. MLNR-DuET

   Plasmid

   #213339

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   MLNR (MLNR Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pM-CBE

   Plasmid

   #196293

   Purpose

   35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP

   Depositor

   Zhenghe Li

   Article

   Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

   Insert

   Full length TSWV M antigenome encoding CBE in place of viral GP
   

   Use

   CRISPR

   Tags

   3×flag

   Expression

   Plant

   Promoter

   duplicated cauliflower mosaic virus 35S promoter

   Available Since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4-myc-HLA-A2

   Plasmid

   #135504

   Purpose

   Mammalian expression of myc-tagged HLA-A*02:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*02:01 (HLA-A Human)
   

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDM304_DPAKa(GBD)-YFP

   Plasmid

   #128699

   Purpose

   expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

   Depositor

   Igor Weber

   Article

   Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

   Insert

   GBD domain (aa 731-890) of DPAKa
   

   Use

   Dictyostelium discoideum expression vector

   Tags

   YFP

   Mutation

   none

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDM358_DPAKa(GBD)-YFP

   Plasmid

   #128700

   Purpose

   expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

   Depositor

   Igor Weber

   Article

   Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

   Insert

   GBD domain (aa 731-890) of DPAKa
   

   Use

   Dictyostelium discoideum expression vector

   Tags

   YFP

   Mutation

   none

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDM326_DPAKa(GBD)-YFP

    Plasmid

    #128701

    Purpose

    expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

    Depositor

    Igor Weber

    Article

    Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

    Insert

    GBD domain (aa 731-890) of DPAKa
    

    Use

    Dictyostelium discoideum expression vector

    Tags

    YFP

    Mutation

    none

    Available Since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-myc-HLA-A3

    Plasmid

    #135505

    Purpose

    Mammalian expression of myc-tagged HLA-A*03:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*03:01 (HLA-A Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB502-omega

    Plasmid

    #74845

    Purpose

    Gateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    2xCaMV35S enhancer and omega

    Available Since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmTurquoise2-Tubulin

    Plasmid

    #36202

    Purpose

    In vivo visualization of microtubules (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    Alpha-tubulin (TUBA1B Human)
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGWB402-omega

    Plasmid

    #74797

    Purpose

    Gateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    2xCaMV35S enhancer and omega

    Available Since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmScarlet-HSP70

    Plasmid

    #163790

    Purpose

    Expresses mScarlet-Hsp70 in mammalian cells

    Depositor

    Vincent Timmerman

    Article

    Adriaenssens et al Sci Rep. 2020 May 29;10(1):8755. doi: 10

    Insert

    HSP70 (HSPA1A Human)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-Hyg-mEGFP

    Plasmid

    #191847

    Purpose

    Expresses the monomeric form of EGFP

    Depositor

    Bertrand Collet

    Article

    Teige et al Front Immunol. 2019 Sep 6;10:2119. doi:

    Insert

    mEGFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFEndoNA2

    Plasmid

    #174667

    Purpose

    Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

    Depositor

    Jukka Finne

    Article

    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

    Insert

    GFP-endoNA2 fusion protein
    

    Tags

    Histag

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs-Stat3-C

    Plasmid

    #13373

    Purpose

    Retroviral expression of dominant active mouse Stat3

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    signal transducer and activator of transcription 3 (dominant active mutant) (Stat3 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Changed Alanine 661 to cysteine and asparagine 66…

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMXS-IP APEX-PEX26

    Plasmid

    #224562

    Purpose

    expresses APEX fused to PEX26 peroxisomal localization sequence in mammalian cells

    Depositor

    Gregory Wyant, William Kaelin

    Article

    Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

    Insert

    PEX26 (PEX26 Human)
    

    Use

    Retroviral

    Tags

    APEX

    Expression

    Mammalian

    Mutation

    PEX26 peroxisomal targeting sequence

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5-FLAG-KPTN

    Plasmid

    #87041

    Purpose

    mammalian expression of KPTN

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    KPTN (KPTN Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAVS1-Puro CAG PSMA nanobody T-CAR

    Plasmid

    #171962

    Purpose

    Donor plasmid for PSMA nanobody T-CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Harris et al J Immunol Regen Med. 2023 May;20:100074.

    Insert

    Synthetic anti-PSMA nanobody CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pRK5-FLAG-C12orf66

    Plasmid

    #87047

    Purpose

    mammalian expression of C12orf66

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    C12orf66 (C12orf66 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRK5-FLAG-ITFG2

    Plasmid

    #87044

    Purpose

    mammalian expression of ITFG2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    ITFG2 (ITFG2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAGM51323

    Plasmid

    #153224

    Purpose

    Level 1 position 2 containing the Arabidopsis Rps5a promoter, Cas9 (Zcasi), and the nos terminator

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    Arabidopsis Rps5a promoter, Cas9 (Zcasi), nos terminator
    

    Use

    Synthetic Biology

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pet28a-His6-Halo-TEV-Nrf2

    Plasmid

    #62455

    Purpose

    Halo-His6 tagged Nrf2 for E.coli expression

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Nrf2 (NFE2L2 Human)
    

    Tags

    His6-Halo-TEV

    Expression

    Bacterial

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCEP4-myc-HLA-B7

    Plasmid

    #135509

    Purpose

    Mammalian expression of myc-tagged HLA-B*07:02

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-B*07:02 (HLA-B Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRK5-myc-Miro1 T18N

    Plasmid

    #47890

    Purpose

    Expresses myc tagged Miro1 T18N dominant negative mutant

    Depositor

    Pontus Aspenström

    Article

    Fransson et al J Biol Chem. 2003 Feb 21;278(8):6495-502

    Insert

    Miro1 T18N (RHOT1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    T18N, dominant negative mutant

    Promoter

    CMV

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S

    Plasmid

    #62458

    Purpose

    expresses his-tagged C151S human Keap1 protein

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Tags

    His-Halo-Tev

    Expression

    Mammalian

    Mutation

    Changed Cysteine 151 of Keap1 to serine

    Promoter

    CMV

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. His-PIMT

    Plasmid

    #34852

    Depositor

    Bruce Downie

    Article

    Dinkins et al Plant J. 2008 Jul;55(1):1-13.

    Insert

    PIMT (PCMT1 Human)
    

    Tags

    6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

30. pET26b_AT118-L-V5-His

    Plasmid

    #222848

    Purpose

    E. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivity

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-L
    

    Tags

    V5-His

    Expression

    Bacterial

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMDC32B-AtMIR390a-B/c

    Plasmid

    #51776

    Purpose

    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor

    Depositor

    James Carrington

    Article

    Carbonell et al Plant Physiol. 2014 Mar 19.

    Insert

    AtMIR390a-B/c
    

    Use

    Plant expression

    Mutation

    A. thaliana MIR390a precursor sequence including …

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. MRGPRD-DuET

    Plasmid

    #213340

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MRGPRD (MRGPRD Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. gWiz_BM40-vGAT-VH-hIgG1-CH1-TS-HIS

    Plasmid

    #218436

    Purpose

    mammalian expression plasmid for the heavy chain fragment of an anti-vGAT Fab domain

    Depositor

    Axel Brunger

    Article

    Gopal et al Curr Res Physiol. 2024 Feb 23;7:100121.

    Insert

    vGAT-Vh-hIgG1-CH1
    

    Use

    Affinity Reagent/ Antibody

    Tags

    BM40 and TwinStrep-6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAGT9050

    Plasmid

    #219429

    Purpose

    PapE Exonuclease fused to linker 4LF2 as N-terminal tag module (AATG_PapE-4*LF2_AGGT)

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    AATG_PapE-4xLF2_AGGT
    

    Use

    Moclo compatible level 0 module

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. NPY1R-DuET

    Plasmid

    #213354

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    NPY1R (NPY1R Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pPLN-hFluc-WT-HA-GFP11-KDEL

    Plasmid

    #177722

    Purpose

    Firefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    hFluc
    

    Tags

    GFP11, HA, KDEL, and Prolactin Signal Sequence

    Expression

    Bacterial and Mammalian

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. BglB in pET29b(+)

    Plasmid

    #55773

    Purpose

    Expresses beta-glucosidase

    Depositor

    Justin Siegel

    Article

    Carlin et al PLoS One. 2016 Jan 27;11(1):e0147596. do

    Insert

    Beta-glucosidase B (bglB Synthetic)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDx_mScarlet-I3-NCwt-SYFP2

    Plasmid

    #189765

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet-I3-NCwt-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-I3-NCwt-SYFP2
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1-ROR2-V5/HIS

    Plasmid

    #205608

    Purpose

    Expression of human ROR2 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    ROR2 receptor tyrosine kinase (ROR2 Human)
    

    Tags

    V5-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. SSTR3-DuET

    Plasmid

    #213378

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    SSTR3 (SSTR3 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge