We narrowed to 12,214 results for: LEA
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Plasmid#181956PurposeGenetically encoded FRET-based cAMP indicator fused to AKAP79.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)
TagsAKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…ExpressionMammalianPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACE WT SOX pACEmam1
Plasmid#192049PurposeExpresses WT SOX (pACEmam1)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertWT SOX
UseUnspecifiedPromoterCMVAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUDP293
Plasmid#204228PurposeEpisomal yeast plasmid expressing the Ercas12a and ErCas12a gRNADepositorInsertsEubacterium rectale cas12a (Ercas12a)
BsaI_GFP drop-out_BsaI
UseSynthetic BiologyTagsNLSMutationcodon optimized for S. cerevisiaePromoterSaccharomyces cerevisiae PGK1 and Saccharomyces c…Available sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
B52_puro_empty_gRNA
Plasmid#197557Purposesites for cloning two gRNA with puromycin cassetteDepositorTypeEmpty backboneUseEmpty grna backbone with puromycin resistance geneExpressionMammalianPromoterU6 for gRNA, CMV for puromycin resistance.Available sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-APEX.NES-P2A-EGFP
Plasmid#182824PurposeCre-dependent expression of cytosolic APEXDepositorInsertAPEX.NES-P2A-EGFP
UseAAVTagsEGFP reporter (P2A)PromoterEF1aAvailable sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-LAP2beta
Plasmid#175112PurposeLentiviral expression of V5-tagged mouse TmpoDepositorAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
TVBB N-term-mTurquoise2
Plasmid#169222PurposeTargeting vector backbone to support a knock-in of mTurquoise2-Linker at the N-terminus of a target locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDouble SapI flanked mTurquoise2
UseTargeting vector backboneAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-LOx-IRES-EGFP
Plasmid#166877PurposeExpress the bacterial lactate oxidase (LOx) enzyme in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLactate oxidase
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB660-CYCD3;1
Plasmid#140751PurposeInducing plant cells to enter mitosisDepositorAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-BCL6
Plasmid#139816Purposedoxycycline-inducible expression of Human BCL6 in mammalian cellsDepositorInsertBCL6 (BCL6 Human)
UseLentiviralExpressionMammalianMutationT190A, E241G, L314M- please see depositor commentsPromoterTRE-mCMVAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a His6Flag2AUF1(p37)
Plasmid#135939PurposeExpresses AUF1p37 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
TagsFlag2 and His6ExpressionBacterialPromoterT7Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-DIO-(STChRger2-TS-EYFP)
Plasmid#129394PurposeSoma Targeted, High photocurrent, low-light sensitive channelrhodopsin (ChRger2) for optogenetic activation with systemic delivery or for low-light activation. Uses the CAG promoter and double floxed.DepositorInsertsoma targeted ChRger2
UseAAV and Cre/LoxTagsKv2.1-TS-EYFPExpressionMammalianPromoterCAGAvailable sinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeA
Plasmid#128943Purposeexpresses PfeA in E. coliDepositorInsertferric enterobactin receptor
TagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75)PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo
Plasmid#112684PurposeAn AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinaseDepositorInsertiRFP713
UseAAVTags2A-Flpo and Nuclear localization signalPromoterCMV-IEAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOG536
Plasmid#105423PurposeSynthetic biologyDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNuclear targeting factor (RanGAP1(WPP)-GFP-BLRP)
UseSynthetic BiologyMutationBsaI/ BpiI restriction sites removedAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprt
Plasmid#92166PurposePlasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vectorDepositorInsertQuinolinate Phosphoribosyltransferase (QPRT Human)
TagsTEV protease -cleavable N-terminal 6HisExpressionBacterialMutationchanged Asp 2 to Asn to create BspHI sitePromoterT7Available sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
NET50 pmRFP-N2 (489)
Plasmid#61983Purposemammalian expression of nuclear envelope transmembrane proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNET50 (DHRS7 Human)
TagsmRFPExpressionMammalianPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DiCre2_59.F2
Plasmid#60209PurposeExpresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)DepositorInsertCre recombinase (CD207 Synthetic, Bacteriophage P1, Human)
TagsFKBP12(F36V) and Nuclear localisation signalExpressionMammalianMutationFKBP12 has the F36V mutation; amino acids 19-59 o…PromoterCMVAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS G_tTA-BFP
Plasmid#58871PurposeMESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorPublicationInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEMS1602
Plasmid#29305PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPle131 (MKI67 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSAvailable sinceNov. 4, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits