We narrowed to 4,899 results for: phy

1. pRetroX-TRE3G-PLOD2_PGK-GpNLuc

   Plasmid

   #136457

   Purpose

   Expresses Tet/Dox-inducible human PLOD2 in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Sato et al Neoplasia. 2021 Jun 6;23(6):594-606. doi

   Insert

   TRE3G::PLOD2-T2A-Hygromycin (PLOD2 Human)
   

   Use

   Luciferase and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

   Plasmid

   #139097

   Purpose

   Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dxCas9-NG
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

   Plasmid

   #129028

   Purpose

   inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA cloning site
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSH-Csy4-T2A-SaRFN

   Plasmid

   #85755

   Purpose

   Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).

   Depositor

   Lawrence Stanton

   Article

   Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

   Insert

   Csy4-T2A-FokI-SadCas9
   

   Use

   CRISPR

   Tags

   3xHA tag, Csy4, and FokI fusion via 36 amino acid…

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   CAG

   Available since

   Jan. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3 2198

   Plasmid

   #49347

   Purpose

   This is actually plasmid pGL3 2185 from the associated publication, which contains a shorter Bmp2 promoter sequence. also contains luciferase reporter.

   Depositor

   Melissa Rogers, Stephen Harris

   Article

   Feng et al Biochim Biophys Acta. 1997 Jan 3;1350(1)

   Insert

   Bmp2 promoter (shorter sequence) (BMP2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   Bmp2 promoter

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FLAG-KIF5A delta Tail

   Plasmid

   #166955

   Purpose

   Expresses FLAG-tagged KIF5A protein (1-820aa) in pcDNA3.1

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   KIF5A (1-820aa)

   Promoter

   CMV

   Available since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCC_07 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9-NLS-VPR-2A-Puro-WPRE

   Plasmid

   #139092

   Purpose

   Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   dxCas9 3.7-VPR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

   Plasmid

   #129036

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA8 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-Flag-PGC-1alpha2

   Plasmid

   #45502

   Depositor

   Jorge Ruas, Bruce Spiegelman

   Article

   Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

   Insert

   PGC-1alpha Isoform2 (Ppargc1a Mouse)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SgmouseTSDR-1

    Plasmid

    #129053

    Purpose

    targeted DNA demethylation_mouse_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting mouse TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA1 (mouseTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-1

    Plasmid

    #129029

    Purpose

    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA1 targeting human TSDR

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA1 (huTSDR)
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. attB-GFP-Tet1d834-1363-Poly(A)

    Plasmid

    #65560

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Tet1d1054-1363 cDNA into a MIN-tagged locus

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    del(834-1363)

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-iLMO (slGLuc-Mac-EYFP)

    Plasmid

    #114100

    Purpose

    fusion protein of Gaussia luciferase variant superluminescent, Leptosphaeria maculans opsin, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    Gaussia luciferase, superluminescent; Leptosphaeria maculans opsin; EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. attB-GFP-Tet1d834-1053-Poly(A)

    Plasmid

    #65561

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Tet1d834-1053 cDNA into a MIN-tagged locus

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    del(834-1053)

    Promoter

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MCUabbb-Flag-PLYS1

    Plasmid

    #236787

    Purpose

    MCU-MCUB fusion in abbb pattern. Only the initial MCU has a mitochondrial targeting sequence.

    Depositor

    Dipayan Chaudhuri

    Article

    Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

    Insert

    MCUB, MCU mixed tetramer (MCU, MCUB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAG8Ha-EWS_RRM(359-447)

    Plasmid

    #188045

    Purpose

    Expresses His8-EWS_RRM(359-447) with a TEV site for removal of the His8 tag.

    Depositor

    David Libich

    Article

    Selig et al Biopolymers. 2023 May;114(5):e23536. doi

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-358, 446-656

    Promoter

    T7

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAG8Ha-EWS_RRM-RGG(359-513)

    Plasmid

    #188046

    Purpose

    Expresses His8-EWS_RRM-RGG(359-513) with a TEV site for removal of the His8 tag.

    Depositor

    David Libich

    Article

    Selig et al Biopolymers. 2023 May;114(5):e23536. doi

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-358, 514-656

    Promoter

    T7

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEmal10H-MBP-EWSR1-449-513

    Plasmid

    #199439

    Purpose

    Expresses RG2 (the second RG-rich region, 449-513) of EWS

    Depositor

    David Libich

    Article

    Selig et al Biopolymers. 2023 May;114(5):e23536. doi

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    Tags

    His10 and MBP

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-448, 514-656

    Promoter

    T7

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-CMV-scFVDnmt3A_bGHpA

    Plasmid

    #177349

    Purpose

    AAV expression of scFV-fused catalytic domain of Dnmt3a for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    catalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    598–908 aa of mouse Dnmt3a

    Promoter

    CMV

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-CMV-scFVDnmt3L_bGHpA

    Plasmid

    #177350

    Purpose

    AAV expression of scFV-fused C terminal domain of Dnmt3l for targeted DNA methylation editing

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Insert

    C-terminal domain of mouse Dnmt3l (Dnmt3l Mouse)
    

    Use

    AAV

    Tags

    myc and scFV

    Expression

    Mammalian

    Mutation

    194–415 aa

    Promoter

    CMV

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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