We narrowed to 7,285 results for: tet

1. pFU-AgaIVA-VG

   Plasmid

   #24145

   Depositor

   Ines Ibanez-Tallon

   Article

   Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

   Insert

   Venus-AgatoxinIVA-FLAG-GPI anchor
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pALS3-Ma-sfGFP-TAG150

   Plasmid

   #212121

   Purpose

   Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

   Depositor

   Ryan Mehl

   Inserts

   sfGFP-150TAG
   M. alvus Pyl-tRNA(6)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   N150TAG

   Promoter

   araC and lpp

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-Ec.coaD (pESC106)

   Plasmid

   #50388

   Purpose

   Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

   Depositor

   Tadhg Begley, Erick Strauss

   Article

   Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

   Insert

   PPAT
   

   Tags

   6xHis-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSECRETS-A

   Plasmid

   #196986

   Purpose

   For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).

   Depositor

   Eric Josephs

   Article

   Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   pltetO1

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Inducible tdTomato

   Plasmid

   #183718

   Purpose

   tdTomato expression from dox-inducible promoter; plasmid encodes the TetR inducer

   Depositor

   Jeffrey Gerst

   Article

   Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

   Inserts

   tdTomato
   rTetR
   

   Use

   Lentiviral

   Promoter

   TRE3GV and UbiC

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. MK1283

   Plasmid

   #71428

   Purpose

   This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709

   Depositor

   Howard Salis

   Article

   Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

   Insert

   Mixed Feedback Loop version of the UBER system
   

   Mutation

   T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …

   Available Since

   Feb. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCL66-decoder

   Plasmid

   #159453

   Purpose

   Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.

   Depositor

   Joerg Stelling

   Article

   Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

   Insert

   Pfus1mut-tetR-nls-malE-tCyc1
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   Pfus1mut

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRosa26 R1-ccdB-R2 RexNeo PI-SceI

   Plasmid

   #24418

   Depositor

   Edward Hsiao

   Article

   Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

   Insert

   
   

   Use

   Mouse Targeting

   Available Since

   March 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. Marathon-RT for Split-PE (pEH59)

   Plasmid

   #190108

   Purpose

   Expresses Marathon-RT (human codon optimization)

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   Marathon-RT
   

   Tags

   bpNLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJBL701

    Plasmid

    #140371

    Purpose

    Expresses TetR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-TetR-T
    

    Tags

    6XHis

    Expression

    Bacterial

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSEVA221AID-T7RNAP

    Plasmid

    #167977

    Purpose

    Expresses fusion protein AID-T7RNAP in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

    Insert

    AID-T7RNAP
    

    Tags

    T7 tag and T7RNAP

    Expression

    Bacterial

    Promoter

    TetR-Ptet

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. MK1274

    Plasmid

    #71431

    Purpose

    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 300 and a TetR translation rate of 35149.

    Depositor

    Howard Salis

    Article

    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.

    Insert

    Mixed Feedback Loop version of the UBER system
    

    Mutation

    T7 RBS:AACCGAGCCCAATATAGGACTCAGGGTGCCAAAAAA and T…

    Available Since

    Dec. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUHD-AID

    Plasmid

    #121066

    Purpose

    vector expresses AID-fusion protein under TRE promoter and with puromycin resistance selection

    Depositor

    Randy Poon

    Article

    Ng et al Cell Cycle. 2018 Dec 23. doi: 10.1080/15

    Type

    Empty backbone

    Tags

    AID

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDB007ns2a

    Plasmid

    #99214

    Purpose

    Accessory plasmid for PACE of PylRS variants; negative selection

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
    

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp, PproK, and Ptet

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS306-PTDH3-SUMO10-6SIM

    Plasmid

    #190248

    Purpose

    Expression SUMO10-SIM6 in yeast cells

    Depositor

    Liam Holt

    Article

    Sang et al Mol Cell. 2022 Oct 6;82(19):3693-3711.e1

    Insert

    10SUMO-6SIM
    

    Expression

    Yeast

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAJM.717

    Plasmid

    #108517

    Purpose

    PTet*-YFP Reporter. aTc sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PTet*-YFP
    

    Expression

    Bacterial

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid

    #212122

    Purpose

    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    

    Tags

    His6 and SUMO

    Expression

    Bacterial

    Promoter

    araC and lpp

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMrfp

    Plasmid

    #59773

    Purpose

    Derived from pCM62; ampR, tetR, PBAD - rfp

    Depositor

    Nathan Hillson

    Article

    Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

    Insert

    rfp
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pREVTRE-AID

    Plasmid

    #121067

    Purpose

    Retrovirus vector expresses AID-fusion protein under TRE promoter and with hygromycin resistance selectio

    Depositor

    Randy Poon

    Article

    Ng et al Cell Cycle. 2018 Dec 23. doi: 10.1080/15

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    AID

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKS014

    Plasmid

    #65467

    Purpose

    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidase

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Insert

    Ntag-beta galactosidase
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Promoter

    pl-TetO

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only