164,313 results

1. pYSD063

   Plasmid

   #212916

   Purpose

   Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCR-YFP-GW

   Plasmid

   #209366

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis SCR promoter driving expression of N-terminal YFP fusion protein.

   Depositor

   Lucia Strader

   Article

   Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWOL-YFP-GW

   Plasmid

   #209365

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis WOL promoter driving expression of N-terminal YFP fusion protein.

   Depositor

   Lucia Strader

   Article

   Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-eIF4E-TwStrep

   Plasmid

   #208638

   Purpose

   for E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)

   Depositor

   Quanfu Ma

   Insert

   human eIF4E (ENST00000450253) (EIF4E Human)
   

   Use

   Tags

   6xHis and Twin Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a MDHM_YEAST

   Plasmid

   #204231

   Purpose

   Bacterial expression of yeast mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDHM_YEAST (MDH1 Budding Yeast)
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Does not include transit peptide and includes a M…

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.4_1G11F2-3 heavy chain

   Plasmid

   #198253

   Purpose

   recombinant expression of heavy chain of monoclonal anti-AMP antibody 1G11F2-3

   Depositor

   Aymelt Itzen

   Article

   Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

   Insert

   heavy chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse IgG2b)
   

   Use

   Tags

   IL10 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAc5.1B-lambdaN-HA-HseIF4AI_T

   Plasmid

   #147543

   Purpose

   Insect Expression of HseIF4AI

   Depositor

   Elisa Izaurralde

   Insert

   HseIF4AI (EIF4A1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   3 silent mutations compared to the sequence given…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD649-HAsp-CEACAM1-COMP5AP-AviTag-9xHis

   Plasmid

   #157390

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   CEACAM1 (CEACAM1 Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFRT/TO/HIS/FLAG/HA-PRDX1

   Plasmid

   #38086

   Depositor

   Markus Landthaler

   Article

   Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

   Insert

   PRDX1 peroxiredoxin 1 (PRDX1 Human)
   

   Use

   Tags

   HIS/FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. piRFP670-N1

    Plasmid

    #45457

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    iRFP670
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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