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We narrowed to 6,189 results for: cas9 expression plasmid

Showing: 6091 - 6120 of 6189 results

  11. scFv-GCN4-DNMT3a(R887E)-DNMT3l

    Plasmid
    #154141
    Purpose
    Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.
    Depositor
    Albert Jeltsch
    Article
    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i
    Insert
    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    Use
    Tags
    HA and sfGFP
    Expression
    Mammalian
    Mutation
    R887E
    Promoter
    SFFV
    Available since
    Oct. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    154141-plasmid-map-sequence-id-345617

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  12. UbC-blasticidine-P2A-TETon-3G transactivator

    Plasmid
    #200191
    Purpose
    A TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    blasticidine - P2A - TETon-3G transactivator
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    UbC
    Available since
    Aug. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    200191-plasmid-map-sequence-id-405986

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  13. pAAV_ACTB CRISPIE

    Plasmid
    #172853
    Purpose
    Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Inserts
    Actb intron 1 sgRNA
    DRS-2 sgRNA
    CRISPIE designer exon (phase 0-0) encoding mEGFP
    mRuby3
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172853-plasmid-map-sequence-id-341002

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  14. CRISPRoff-mScarletI

    Plasmid
    #217783
    Purpose
    Expresses CRISPRoff-mScarletI (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-mScarletI-KRAB) downstream of the CAG promoter for gene epigenetic silencing
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    CRISPRoff-mScarletI (DNMT3A-DNMT3L-dCas9-mScarletI-KRAB)
    Use
    Tags
    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and mScarletI
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    217783-plasmid-map-sequence-id-468886

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  15. DiLiCre 2.0

    Plasmid
    #198752
    Purpose
    A Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    Cre-PhoCI-ERT2-ERT2
    Use
    Lentiviral
    Tags
    Cre, kept in a photocleavable site, followed by 2…
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    TRE3GV
    Available since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198752-plasmid-map-sequence-id-397647

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  17. pMT1-13X-NOG(AtUBI10)-MTAP-LUC

    Plasmid
    #234371
    Purpose
    T-DNA vector for expression of the the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs, no gRNA included (NOG)
    Depositor
    Michael Smanski
    Article
    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70
    Inserts
    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-13XGP41
    Use
    Synthetic Biology
    Tags
    GB1, GP41, and sfGFP
    Expression
    Plant
    Mutation
    Promoter
    Arabidopsis Ubi10 and MTAP1 (synthetic)
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234371-plasmid-map-sequence-id-469425

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  30. pCRISPR-cBEST-v2-SF14P

    Plasmid
    #209448
    Purpose
    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoter
    Depositor
    Tilmann Weber
    Article
    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021
    Insert
    Codon optimized APOBEC1-nCas9-UGI fusion protein
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PtipA; sgRNA expression from SF14P promoter
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209448-plasmid-map-sequence-id-416625

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Showing: 6091 - 6120 of 6189 results