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pAc-sgRNA-Cas9.dora_1

Plasmid

#190700

Purpose

Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

dora (CG34401) sgRNA 1

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.dora_2

Plasmid

#190701

Purpose

Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

dora (CG34401) sgRNA 2

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAc-sgRNA-Cas9.dora_3

Plasmid

#190702

Purpose

Expresses sgRNA targeting Dora gene and Cas9-Puro in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

dora (CG34401) sgRNA 3

Use

CRISPR

Expression

Insect

Promoter

Drosophila U6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NLS-QPAS1-VP16

Plasmid

#186187

Purpose

AAV vector packaging QPAS1-VP16, a component of optogenetic system for NIR light-controllable transcriptional activation

Depositor

Vladislav Verkhusha

Article

Kasatkina et al Nat Commun. 2022 May 19;13(1):2813. doi:

Insert

NLS-QPAS1-VP16

Use

AAV

Promoter

CAG

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pL0-NbPT5bPro

Plasmid

#159416

Purpose

Nicotiana benthamiana PT5b promoter sequence (1068 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

NbPT5b Promoter

Use

Puc19-derived

Expression

Bacterial

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pL0-NbBCP1bPro

Plasmid

#159417

Purpose

Nicotiana benthamiana BCP1b promoter sequence (1231 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

NbBCP1b Pro

Use

Puc19-derived

Expression

Bacterial

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pRS314-U6-G96C/A97C/U100C/U101C

Plasmid

#82328

Purpose

U6 telestem mutant (G96C/A97C/U100C/U101C) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314-U6-A34G/A35G/G96C/A97C

Plasmid

#81238

Purpose

U6 telestem mutant (A34G/A35G/G96C/A97C) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with A34G/A35G/G96C/A97C mutation (snR6 Budding Yeast)

Expression

Yeast

Mutation

U6 telestem mutant (A34G/A35G/G96C/A97C)

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0015

Plasmid

#117551

Purpose

Level 1 Golden Gate Cassette: SaCas9 expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + SaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)

Expression

Plant

Promoter

2x35S+5'UTR OMEGA (pICH51288)

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK502

Plasmid

#121457

Purpose

CEN HIS3 CNB1

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Promoter

Endogenous genomic promoter region

Available Since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK513

Plasmid

#121470

Purpose

CEN LEU2 CNB1mutEF1

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF1 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Mutation

Changed aspartic acid 34 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK511

Plasmid

#121468

Purpose

2µm HIS3 CNB1mutEF4

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF4 (CNB1 Budding Yeast)

Use

Yeast high copy 2 micron vector

Mutation

Changed aspartic acid 144 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTJK510

Plasmid

#121465

Purpose

2µm HIS3 CNB1mutEF3

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF3 (CNB1 Budding Yeast)

Use

Yeast high copy 2 micron vector

Mutation

Changed aspartic acid 103 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTJK509

Plasmid

#121464

Purpose

2µm HIS3 CNB1mutEF2

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF2 (CNB1 Budding Yeast)

Use

Yeast high copy 2 micron vector

Mutation

Changed aspartic acid 66 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pTJK508

Plasmid

#121463

Purpose

2µm HIS3 CNB1mutEF1

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF1 (CNB1 Budding Yeast)

Use

Yeast high copy 2 micron vector

Mutation

Changed aspartic acid 34 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTJK516

Plasmid

#121473

Purpose

CEN LEU2 CNB1mutEF4

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF4 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Mutation

Changed aspartic acid 144 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTJK506

Plasmid

#121461

Purpose

CEN HIS3 CNB1mutEF4

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF4 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Mutation

Changed aspartic acid 144 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTJK505

Plasmid

#121460

Purpose

CEN HIS3 CNB1mutEF3

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF2 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Mutation

Changed aspartic acid 103 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTJK504

Plasmid

#121459

Purpose

CEN HIS3 CNB1mutEF2

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF2 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Mutation

Changed aspartic acid 66 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTJK503

Plasmid

#121458

Purpose

CEN HIS3 CNB1mutEF1

Depositor

Tami Kingsbury

Article

Connolly et al Genetics. 2018 Jul;209(3):801-813. doi:

Insert

CNB1mutEF1 (CNB1 Budding Yeast)

Use

Yeast cen plasmid

Mutation

Changed aspartic acid 34 to alanine

Promoter

Endogenous genomic promoter region

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge