We narrowed to 5,494 results for: SPI

1. CYFIP2.mCherry

   Plasmid

   #122052

   Purpose

   Expresses human CYFIP2 in mammalian cells.

   Depositor

   Josef Kittler

   Article

   Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

   Insert

   CYFIP2 (CYFIP2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lentiviral shRNA GEF-H1

   Plasmid

   #21477

   Depositor

   Richard Huganir

   Article

   Kang et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

   Insert

   rho/rac guanine nucleotide exchange factor (GEF) 2 (Arhgef2 Rat)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA5-FRT/TO-LacR-TopBP1

   Plasmid

   #31313

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

   Insert

   TopBP1 (TOPBP1 Human)
   

   Use

   Tags

   FLAG and LacR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pYPQ230-RVQ

   Plasmid

   #200187

   Purpose

   Gateway entry clone (attL1 & attR5) for LbCas12a-RVQ mediated genome editing

   Depositor

   Yiping Qi

   Article

   Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

   Insert

   LbCas12a-RVQ
   

   Use

   CRISPR; Gateway compatible lbcas12a-rv entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   G146R, R182V, E795Q

   Promoter

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBLO ddd64.1

   Plasmid

   #123121

   Purpose

   E. coli expression vector for catalytically inactivated deltaproteobacteria CasX

   Depositor

   Benjamin Oakes, Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   CasX
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Mutated amino acids in catalytic triad to alanine…

   Promoter

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-dCas9-MQ1(S317A)-EGFP

   Plasmid

   #89636

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR

   Tags

   3*FLAG and 6*His

   Expression

   Mammalian

   Mutation

   S317A, AGC-->GCC

   Promoter

   CMV

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSP64T-hLbCpf1

   Plasmid

   #107187

   Purpose

   plasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)

   Depositor

   Antonio Giraldez

   Article

   Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

   Insert

   hLbCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mutation

   Promoter

   sp6

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p-mCherry2-sgMUC4

   Plasmid

   #198399

   Purpose

   Expresses a guide RNA against a repetitive locus found in the MUC4 gene of human chromosome 3, with mCherry2 reporter

   Depositor

   Sarah McClelland

   Article

   Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

   Insert

   MUC4 sgRNA (MUC4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA 3.1-nsp7-FLAG

   Plasmid

   #201027

   Purpose

   Expresses C-terminally FLAG tagged SARS-CoV-2 nsp7 in mammalian.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   non-structural protein 7 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   8xHis and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTriEX-Antenna-GDI RhoA

    Plasmid

    #189669

    Purpose

    Expression of Antenna-RhoA

    Depositor

    Klaus Hahn

    Article

    Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CB6-GFP-TAOK2 (K57A)

    Plasmid

    #197114

    Purpose

    Expression of kinase-deficient GFP-tagged TAOK2 (K57A) / PSK1 (K57A) in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

    Insert

    TAOK2 (K57A) (TAOK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed K 57 to A

    Promoter

    CMV

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDH-EFS-FlpO

    Plasmid

    #191509

    Purpose

    Lentiviral Flp Recombinase driven by the EFS promoter

    Depositor

    Eric Snyder

    Article

    Orstad et al Dev Cell. 2022 Aug 8;57(15):1866-1882.e1

    Insert

    flp recombinase (flp )
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/EFS promoter inserted

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SARS_COV2_ORF3b_HA_BASU

    Plasmid

    #153992

    Purpose

    Express Orf3b gene along with HA and BASU

    Depositor

    Paul Khavari

    Article

    Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

    Insert

    orf3b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CoV-2-NSP3-c001

    Plasmid

    #173083

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E1084-E1192, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mCherry-PH-SWAP70

    Plasmid

    #84582

    Purpose

    Expresses mCherry-tagged PH-domain of SWAP70 in mammalian cells, binds to PI(3,4)P2

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

    Insert

    SWAP70 residues 209-306 (SWAP70 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Pleckstrin-homology domain, residues 209-306

    Promoter

    CMV

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. CB6-GFP-TAOK1

    Plasmid

    #197108

    Purpose

    Expression of GFP-tagged TAOK1 / PSK2 in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

    Insert

    TAOK1 (TAOK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Wild Type

    Promoter

    CMV

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pT7-7 asyn S129A

    Plasmid

    #36048

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

    Insert

    alpha-synuclein S129A (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S129A

    Promoter

    T7

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTriEX-Antenna-GDI Cdc42

    Plasmid

    #101681

    Purpose

    "Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al

    Depositor

    Klaus Hahn

    Article

    Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

    Insert

    Cdc42
    

    Use

    Tags

    6xHis and FRET Antenna (mCerulean-cpVenus)

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-dCpf1-BE-YE

    Plasmid

    #107686

    Purpose

    Expresses dLbCpf1-BE-YE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE-YE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y/R126E), dLbCpf1(D832A/E925A/D1148A)

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRetro-XT-PAK2-CFP(puro)-WT

    Plasmid

    #81176

    Purpose

    Retroviral vector for expression of wild-type PAK2 with a CFP fusion

    Depositor

    Angeliki Malliri

    Article

    Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

    Insert

    p21 (RAC1) activated kinase 2 (PAK2 Human)
    

    Use

    Retroviral

    Tags

    CFP

    Expression

    Mutation

    Promoter

    P-tight TRE

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only