We narrowed to 5,566 results for: SPI

1. 4xnrUAS:MLS-KillerRed in pTol2pA2-acrys-EGFP-

   Plasmid

   #115516

   Purpose

   Plasmid driving expression of membrane bound KillerRed fluorophore under UAS response element, with a green lens reporter

   Depositor

   Marco Morsch

   Article

   Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

   Insert

   MLS-KillerRed
   

   Use

   Gateway cloning

   Tags

   MLS

   Expression

   Mutation

   Promoter

   UAS

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-mEGFP-CaMKIIa

   Plasmid

   #127389

   Purpose

   Fluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alpha

   Depositor

   Ryohei Yasuda

   Article

   Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-UBC-hAAT-IRES-ZsGreen-W

   Plasmid

   #126702

   Purpose

   lentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreen

   Depositor

   Darrell Kotton

   Article

   Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

   Insert

   Alpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   UBC

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEGFP-HRas G12V

   Plasmid

   #18666

   Depositor

   Karel Svoboda

   Article

   Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

   Insert

   H-Ras (HRAS Human)
   

   Use

   Tags

   monomeric EGFP

   Expression

   Mammalian

   Mutation

   G12V mutation in HRas

   Promoter

   Available since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-hACE2-LgBiT

   Plasmid

   #215837

   Purpose

   Expresses hACE2 receptor with LgBiT tag in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   hACE2-LgBiT (ACE2 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-hSYN-eGFP

   Plasmid

   #162512

   Purpose

   AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (hSYN) promoter

   Depositor

   Bart Nieuwenhuis

   Article

   Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSYN

   Available since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ230-RRV

   Plasmid

   #188543

   Purpose

   Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing

   Depositor

   Yiping Qi

   Article

   Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

   Insert

   LbCas12a-RRV
   

   Use

   CRISPR; Gateway compatible lbcas12a-rrv entry clo…

   Tags

   NLS

   Expression

   Plant

   Mutation

   G146R, D156R, R182V

   Promoter

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD_sfGFP3TCG4TAG

   Plasmid

   #174518

   Purpose

   Recoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.

   Depositor

   Jason W Chin

   Article

   Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

   Insert

   sfGFP
   

   Use

   Tags

   6 x His

   Expression

   Bacterial

   Mutation

   Recoded. TCG and TAG codons were introduced in po…

   Promoter

   araBAD

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Stargazin-GFP-LOVpep

   Plasmid

   #80406

   Purpose

   Membrane localized GFP-tagged LOVpep. Expresses in mammalian cells

   Depositor

   Michael Glotzer

   Article

   Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

   Insert

   Stargazin-GFP-LOVpep (CACNG2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP PICH (Nigg CB62)

    Plasmid

    #41163

    Depositor

    Erich Nigg

    Article

    Baumann et al Cell. 2007 Jan 12;128(1):101-14.

    Insert

    PICH (ERCC6L Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. T/Brachyury-eGFP Rex Neo

    Plasmid

    #21222

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Brachyury promoter
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. BDNF-SEP

    Plasmid

    #83955

    Purpose

    BDNF with pH sensitive fluorophore for visualization of release

    Depositor

    Ryohei Yasuda

    Article

    Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

    Insert

    Brain derived neurotrophic factor (Bdnf Mouse)
    

    Use

    Tags

    SEP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. mouse CENP-B-EGFP

    Plasmid

    #107264

    Purpose

    mouse CENP-B-EGFP in pIVT vector for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Akera et al Science. 2017 Nov 3;358(6363):668-672. d

    Insert

    CENP-B-EGFP (Cenpb Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQCXIP EGFP-ER [TM(SAC1)]

    Plasmid

    #186475

    Purpose

    Expression of the Endoplasmic Reticulum marker GFP-ER [TM(SAC1)] in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

    Insert

    SAC1 (SACM1L Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C-terminal transmembrane region of the protein: 5…

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST15 hSlp4-a

    Plasmid

    #40046

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp4-a (SYTL4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

16. EF1a-LCB1_v1.3-PDGFR-T2A-mCherry (pZYW048)

    Plasmid

    #194231

    Purpose

    Lentiviral expression vector expressing LCB1 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    EF1a-LCB1-PDGFR TMD-T2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. ePop

    Plasmid

    #50953

    Purpose

    Expresses E lysis protein that causes in subpopulation death when threshold cell density is reached, resulting in oscillations

    Depositor

    Lingchong You

    Article

    Marguet et al PLoS One. 2010 Jul 30;5(7):e11909. doi:

    Inserts

    E lysis protein
    LuxI
    LuxR
    

    Use

    Tags

    Expression

    Mutation

    Early stop codon introduced in mutated luxR resul…

    Promoter

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET 2C-T10-dCasX

    Plasmid

    #126418

    Purpose

    E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    DpbCasX
    

    Use

    CRISPR

    Tags

    His-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-NT-3/D15A

    Plasmid

    #73035

    Purpose

    Express an NT-3-BDNF hybrid neurotrophic factor in mammalian cells

    Depositor

    Pantelis Tsoulfas

    Article

    Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

    Insert

    NT-3/D15A (NTF3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Mutated Aspartic acid in position 15 of the matur…

    Promoter

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAT003

    Plasmid

    #171628

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-TRAC
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1-a

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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