We narrowed to 83,595 results for: TRI

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Ube1/PET21d

Plasmid

#34965

Depositor

Cynthia Wolberger

Article

Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

Insert

Ube1 (UBA1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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L1_CsR-Ck1

Plasmid

#136124

Purpose

L1 in position 1 (next to LB in L2 construct), for CsR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35Sx2:mALS_3t35S

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CAP-AAV.FUS.3

Plasmid

#222333

Purpose

AAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with tropism for CNS neurons upon focused ultrasound mediated delivery.

Depositor

Mikhail Shapiro, Jerzy Szablowski

Article

Li et al Nat Commun. 2024 Jun 10;15(1):4924. doi:

Insert

AAV9 modified with 7-amino acid insertion between AA position 588 and 589 of VP1

Use

AAV

Promoter

p41

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCk3_spacer

Plasmid

#136073

Purpose

Use when making even level constructs (L2,...) if pCk3 position (position 3) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-IRES-Gβγ-BERKY3

Plasmid

#158426

Purpose

BRET biosensor for detection of free Gβγ in lentiviral vector pLVX-IRES-Hyg

Depositor

Mikel Garcia-Marcos

Article

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct

Use

Lentiviral

Promoter

CMV

Available Since

Sept. 17, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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ER-RpHLuorin2

Plasmid

#171717

Purpose

Luminal ER expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 targeted to ER

Tags

KDEL ER-retention signal and Signal sequence of h…

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCsA

Plasmid

#136067

Purpose

Acceptor plasmid for even level position 1, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pFG4

Plasmid

#87251

Purpose

gene for resistance to hygromycin (Hyg) inserted next to the parS segments (INT). Also known as pANCH2-Hph

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

Hyg

Tags

parS segments

Expression

Yeast

Promoter

TEF

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-mito-Citroff1-S245

Plasmid

#134306

Purpose

mitochondria-targeted inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 S245A

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_30bpLinker

Plasmid

#157789

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with 30 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_30bp linker

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pFG2

Plasmid

#87250

Purpose

gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT). Also known as pANCH1-Nat

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

Nat

Tags

parS segments

Expression

Yeast

Promoter

TEF

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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MARCKS(1-40)-paGFP

Plasmid

#18695

Depositor

Karel Svoboda

Article

Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

Insert

MARCKS(1-40) (MARCKS Human)

Tags

photoactivatable GFP

Expression

Mammalian

Mutation

A206K mutation in paGFP

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1+ HA-hAO7

Plasmid

#137840

Purpose

N-terminal tagged human AO7 (aa 2-439) for expression in mammalian cells

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 (RNF25 Human)

Tags

HA

Expression

Mammalian

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.Cre

Plasmid

#170367

Purpose

AAV vector expressing Cre under human synapsin promotor

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

Cre

Use

AAV

Promoter

human Synapsin

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-6-TaGRF-GIF-9

Plasmid

#197747

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

TaGRF-GIF chimeric protein CDS

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-Hb9-GFP

Plasmid

#37080

Purpose

3rd generation lentiviral transfer plasmid for Hb9 GFP

Depositor

Fred Gage

Article

Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

Insert

Hb9 GFP

Use

Lentiviral

Promoter

Hb9

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pDSW1728

Plasmid

#120812

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)

Depositor

Craig Ellermeier, David S. Weiss

Article

Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

Insert

red fluorescent protein (mCherry), codon-optimized for C. difficile

Expression

Bacterial

Promoter

Ptet

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_12GLI-FLuc_R4-R3

Plasmid

#113712

Purpose

Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

12GLI-FLuc

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPER-scramble

Plasmid

#118349

Purpose

This plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

scrambled shRNA

Expression

Mammalian

Mutation

WT

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTNT-B18R-6His

Plasmid

#58979

Purpose

expresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

B18R

Tags

His

Expression

Bacterial

Promoter

T7/Sp6

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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