We narrowed to 17,568 results for: bon;

1. pEGFP-C3-PLK4-S305A-3xFLAG

   Plasmid

   #69839

   Purpose

   This plasmid encodes PLK4 isoform 1 carrying an S305A mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

   Depositor

   Michel Bornens

   Article

   Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

   Insert

   PLK4 (PLK4 Human)
   

   Tags

   3xFLAG tag and EGFP

   Expression

   Mammalian

   Mutation

   S305A

   Promoter

   CMV

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT/TO/HIS/FLAG/HA-MYEF2

   Plasmid

   #38069

   Depositor

   Markus Landthaler

   Article

   Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

   Insert

   MYEF2 myelin expression factor 2 (MYEF2 Human)
   

   Tags

   HIS/FLAG/HA

   Expression

   Mammalian

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMS1725

   Plasmid

   #105870

   Purpose

   High copy number plasmid containing iCreERT2-FFrt-KanR-FFrt insert

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   iCreERT2-FFrt-KanR-FFrt
   

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGLTR-S-PURO

   Plasmid

   #58244

   Purpose

   GATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven Puromycin resistance

   Depositor

   Stephan Geley

   Article

   Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

   Inserts

   attR1-Cm(R)-CcdB-attR2
   Puro(R)
   

   Use

   Lentiviral and RNAi; Gateway destination vector

   Expression

   Mammalian

   Promoter

   SFFV and no

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

   Plasmid

   #124660

   Purpose

   reporter protein which emits bright blue fluorescence

   Depositor

   Yutaka Kuroda

   Article

   Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

   Insert

   Gaussia luciferase-C9D-TGinsert
   

   Tags

   IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

   Expression

   Bacterial

   Mutation

   changed glutamic acid 100 to alanine, and glycine…

   Promoter

   T7 promoter

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSK+ mBMPR-1B DN

   Plasmid

   #49530

   Purpose

   cloning vector for dominant negative mouse BMP receptor 1B

   Depositor

   Lee Niswander, Peter ten Dijke

   Article

   Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

   Insert

   BMPR-1B DN (Bmpr1b Mouse)
   

   Expression

   Bacterial

   Mutation

   dominant negative mutation (K231R); also L2V

   Promoter

   T3

   Available Since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-VTKD3

   Plasmid

   #170848

   Purpose

   AAV backbone for Flp-dependent transgene expression of any transgene in cortical interneurons under the control of the Dlx enhancer.

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Frt-hspB9

   Plasmid

   #63100

   Purpose

   Expression of HSPB9 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb9 (HSPB9 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUPD2 PhiC31 integrase (GB1496)

   Plasmid

   #160578

   Purpose

   Streptomyces phage PhiC31 integrase, complete CDS

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   PhiC31
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-C3-PLK4-S305E-3xFLAG

    Plasmid

    #69840

    Purpose

    This plasmid encodes PLK4 isoform 1 carrying an S305E mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    S305E

    Promoter

    CMV

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT

    Plasmid

    #140201

    Purpose

    Fungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology; Cloning of crrna an…

    Tags

    dLbCas12a crRNA scaffold

    Promoter

    PgpdA

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. JPF0535a

    Plasmid

    #124042

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C3-PLK4-S285A/T289A-3xFLAG

    Plasmid

    #69841

    Purpose

    This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    S285A/T289A

    Promoter

    CMV

    Available Since

    Dec. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGAPTrap-GFP

    Plasmid

    #82506

    Purpose

    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH.

    Depositor

    Ed Stanley

    Article

    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEMS2044

    Plasmid

    #79652

    Purpose

    ssAAV genome with Ple255 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    ssAAV Ple255-EmGFP WPRE
    

    Use

    AAV

    Promoter

    PAX6 HS234Z Enhancer

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)

    Plasmid

    #160616

    Purpose

    TU for the constitutive expression of Streptomyces phage PhiC31 integrase.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Frt-hspB6

    Plasmid

    #63097

    Purpose

    Expression of HSPB6 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb6 (HSPB6 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. Frt-hspB4

    Plasmid

    #63095

    Purpose

    Expression of HSPB4 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb4 (CRYAA Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-FLAG-NEMO-C54/347A

    Plasmid

    #27268

    Depositor

    Thomas Gilmore

    Article

    Herscovitch et al Biochem Biophys Res Commun. 2008 Feb 29.

    Insert

    NEMO (IKBKG Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Cys54Ala Cys347Ala

    Available Since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pEMS2049

    Plasmid

    #79657

    Purpose

    ssAAV genome with Ple260 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    ssAAV Ple260-EmGFP WPRE
    

    Use

    AAV

    Promoter

    PAX6 Retinal Enhancer

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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