We narrowed to 6,070 results for: nls

1. GB1531

   Plasmid

   #215242

   Purpose

   TU for constitutive expression of Streptomyces phage phiC31 integrase. Catalyzes site-specific recombination between phiC31 attB and attP sites. Contains SV40 NLS. N. benthamiana codon optimized.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   PNos:PhiC31:TNos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKL2-138

   Plasmid

   #219413

   Purpose

   Estradiol-inducible expression of 42-10 Zinc finger activator

   Depositor

   Ahmad Khalil

   Article

   Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

   Insert

   {pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Four Arg to Ala mutations in zinc finger backbone

   Promoter

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ262F-ABE8e

   Plasmid

   #199179

   Purpose

   Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e-HF mediated A-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Plant Physiol. 2023 Aug 31;193(1):291-30

   Insert

   ABE8e(V106W)-zSpCas9(D10A)
   

   Use

   CRISPR; Gateway compatible abe8e(v106w)-zspcas9(d…

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSmart-EF1alpha-NlaCas7-p65

   Plasmid

   #207394

   Purpose

   Expresses NlaCas7-p65 activator fusion in mammalian cells

   Depositor

   Yan Zhang

   Article

   Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

   Insert

   EF1a-NlaCas7-p65-bGH polyA
   

   Use

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AV13_pCAG.Cas9.gRNA.NT

   Plasmid

   #199260

   Purpose

   Expression construct encoding SpCas9 nuclease and a control non-targeting guide RNA

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Inserts

   SpCas9 nuclease
   Non-targeting (NT) control gRNA
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter and RNA polymerase III promoter for …

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ265N1

   Plasmid

   #173998

   Purpose

   Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-UNG for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   rAPOBEC1-zCas9(D10A)-UNG
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   D10A

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ265O1

   Plasmid

   #173999

   Purpose

   Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   rAPOBEC1-zCas9(D10A)-rXRCC1
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   D10A

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. ppBAalbNixE1E3E4 PUb-YFP

   Plasmid

   #173666

   Purpose

   transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

   Inserts

   Nix masculinisation gene
   YFP
   

   Use

   Tags

   SV40 NLS

   Expression

   Insect

   Mutation

   Nix isoforms with exons E1, E3, E4

   Promoter

   Aedes aegypti PUb and own

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. EC2_2_dCas9_VP64_sgRNA

   Plasmid

   #163707

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   VP64+SV40 NLS
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJT140_GalL_cCDA1-VRERBE3

    Plasmid

    #145103

    Purpose

    Expressing base editor cCDA1-VRERBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-VRERBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

    Promoter

    GalL

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJT46_GalL_cCDA1-BE3

    Plasmid

    #145039

    Purpose

    Expresses cCDA1-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    cCDA1-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJT176_GalL_eA3A-BE3

    Plasmid

    #145125

    Purpose

    Expressing base editor eA3A-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A(N57G)-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(N57G); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJT171_GalL_A3AΔ178-BE3

    Plasmid

    #145121

    Purpose

    Expressing base editor A3AΔ178-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3AΔ178-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(1-178AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJT170_GalL_A3AΔ182-BE3

    Plasmid

    #145120

    Purpose

    Expressing base editor A3AΔ182-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3AΔ182-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(1-182AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJT169_GalL_A3AΔ186-BE3

    Plasmid

    #145119

    Purpose

    Expressing base editor A3AΔ186-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3AΔ186-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(1-186AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJT166_GalL_A3A-BE3

    Plasmid

    #145116

    Purpose

    Expressing base editor A3A-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJT139_GalL_nCDA1-VRERBE3

    Plasmid

    #145102

    Purpose

    Expressing base editor nCDA1-VRERBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    nCDA1-VRERBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJT122_GalL_cCDA1-VQRBE3

    Plasmid

    #145087

    Purpose

    Expressing base editor cCDA1-VQRBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-VQRBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A, D1135V, R1335Q, T1337R)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJT92_GalL_nCDA1Δ188-BE3

    Plasmid

    #145052

    Purpose

    Expresses nCDA1Δ188-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ188-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-188AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJT90_GalL_nCDA1Δ190-BE3

    Plasmid

    #145050

    Purpose

    Expresses nCDA1Δ190-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ190-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-190AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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