We narrowed to 5,415 results for: PEP;

1. pOCC28

   Plasmid

   #118873

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease and MBP, cleavab…

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

   Plasmid

   #183605

   Purpose

   Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Insert

   EGFP-TM
   

   Use

   Tags

   HA, Human PDGFRB transmembrane domain, Mouse IgGK…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF NS34a-msGFP-'S-mito

   Plasmid

   #158765

   Purpose

   Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

   Depositor

   Edwin Chapman

   Article

   Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

   Insert

   NS3/4a protease and msGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Flexible linkers between the protease and msGFP a…

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-BaLgp160-754*

   Plasmid

   #123212

   Purpose

   Mammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncation

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (BaL) Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; Premature stop co…

   Promoter

   CMV

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJRoC30_VP5H

   Plasmid

   #188061

   Purpose

   PROSS-designed versatile peroxidase 5H

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

   Insert

   PROSS-designed versatile peroxidase from Pleurotus ostreatus
   

   Use

   Tags

   S. cerevisiae native α-factor prepro-leader signa…

   Expression

   Yeast

   Mutation

   43 PROSS mutations, described in publication

   Promoter

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 35S::Cel1SP-GeNL/pCAMBIA1301

   Plasmid

   #190069

   Purpose

   Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

   Depositor

   Takeharu Nagai

   Article

   Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

   Insert

   Cel1 signal peptide-Green-enhanced Nano-lantern
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYSD141

   Plasmid

   #212928

   Purpose

   Encodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   mRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. ptdEos-CLC

   Plasmid

   #38009

   Depositor

   Xiaowei Zhuang

   Article

   Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

   Inserts

   Clathrin, light polypeptide (LCa) (Clta Mouse)
   Eos FP, tandem dimer
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOCC267

   Plasmid

   #118887

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tag

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6-MBP, cleavable with 3C protease; HALO tag

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. CD200RCd4d3+4-bio

    Plasmid

    #32406

    Purpose

    A positive control receptor bait (rat CD4d3+4-bio) and prey (rat CD200 receptor) EXPRESs plasmid for The Basic AVEXIS kit

    Depositor

    Gavin Wright

    Article

    Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

    Insert

    CD200 Receptor extracellular domain (Cd200r1 Rat)
    

    Use

    Tags

    Cd4d3+4 and biotinylation peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-3xHAtag-SP-PTPRO

    Plasmid

    #191696

    Purpose

    For expression coding sequencing of PTPRO

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    signal peptide, 3xHAtag and full length coding sequence of PTPRO
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PGRMC2-CD4d3+4-bio

    Plasmid

    #73112

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding PGRMC2 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    PGRMC2 (PGRMC2 Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKG209

    Plasmid

    #45109

    Depositor

    Iain Cheeseman

    Article

    Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

    Insert

    CENP-T (aa1-374) Laci-NLS (CENPT Human)
    

    Use

    Tags

    GFP, Laci-NLS, s peptide, and tev

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENN.AAV.CB7.CI.PM20D1-flag.WPRE.rBG

    Plasmid

    #132682

    Purpose

    AAV plasmid encoding mouse PM20D1 with C-terminal flag tag

    Depositor

    Jonathan Long

    Article

    Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

    Insert

    Peptidase M20 domain containing 1 (Pm20d1 Mouse)
    

    Use

    AAV

    Tags

    6xHIS and Flag

    Expression

    Mutation

    Promoter

    Chicken b-actin

    Available since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSG5-Cyp26a1

    Plasmid

    #13457

    Depositor

    T. Michael Underhill

    Article

    Hoffman et al J Cell Biol. 2006 Jul 3. 174(1):101-13.

    Insert

    Cyp26a1 (Cyp26a1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pET28a-HsSENP3(307-574)

    Plasmid

    #71481

    Purpose

    Encodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).

    Depositor

    Jorge Eduardo Azevedo

    Article

    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

    Insert

    SUMO1/sentrin specific peptidase 3 (SENP3 Human)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1-306 (catalytic domain only)

    Promoter

    T7

    Available since

    Dec. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pQE80L TriCatcher-RGDSP-MMP

    Plasmid

    #112633

    Purpose

    Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    TriCatcher-RGDSP-MMP
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    central matrix metalloproteinase-cleavable sequen…

    Promoter

    T5

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA_DiCre_59.F2

    Plasmid

    #60207

    Purpose

    Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)

    Depositor

    Jean-Paul Herman

    Article

    Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

    Insert

    Cre recombinase (CD207 Human, Bacteriophage P1, Synthetic)
    

    Use

    Tags

    FKBP12 and Nuclear localisation signal

    Expression

    Mammalian

    Mutation

    amino acids 19-59 of the humanized version of Cre…

    Promoter

    CMV

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-SP-CLSTN2-3xHAtag

    Plasmid

    #191693

    Purpose

    For expression coding sequencing of CLSTN2

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    signal peptide, full length coding sequence of CLSTN2 and 3xHAtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOCC167

    Plasmid

    #118909

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HBMss-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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