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GGE094

Plasmid

#120787

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assembly

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

InsDown_Zeta-SfiI

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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GGE091

Plasmid

#120786

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assembly

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

InsUp_Zeta-SfiI

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBT374_pattB-pCA-GFP-pA-attBSwa

Plasmid

#52553

Purpose

for PhiC31 integrase-mediated cassette exchange

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pCfB8382

Plasmid

#126916

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pTV-Fbx15

Plasmid

#13464

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

IRES-Bgeo-pA cassette flanked by Fbx15 homologous arms

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pDDIT3-donor

Plasmid

#112300

Purpose

CRISPR donor plasmid to tag human transcription factor DDIT3 with GFP

Depositor

Kevin White

Insert

DDIT3 homology arms flanking EGFP-IRES-Neo cassette (DDIT3 Human)

Use

CRISPR

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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GGE254

Plasmid

#120789

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

InsDown_Lip-NotI

Use

Cloning/assembly plasmid

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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GGE255

Plasmid

#120788

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

InsUp_Lip-NotI

Use

Cloning/assembly plasmid

Available Since

Nov. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pHOXB5-donor

Plasmid

#113815

Purpose

CRISPR donor plasmid to tag human transcription factor HOXB5 with GFP

Depositor

Kevin White

Insert

HOXB5 homology arms flanking EGFP-IRES-Neo cassette (HOXB5 Human)

Use

CRISPR

Mutation

rs2288276, rs7406798, rs36049616, rs3087996

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGPS21loxFRTNeo

Plasmid

#27180

Depositor

Yoh Wada

Article

Aoyama et al Nucleic Acids Res. 2005 . 33(5):e52.

Insert

lox/Frt/-PGK/EM7pm-G418/Kan-lox/Frt cassette flanked by Tn7L/R

Use

In vitro transposion

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

Plasmid

#162810

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_Intein_DD

Use

Piggybac

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pOVC1_L3(GSSSSGGSSSG)

Plasmid

#131210

Purpose

Cloning vector containing A-B-C casette with linker L3 in site B

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

GSSSSGGSSSG llinker

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_L2(GSSGGSSG)

Plasmid

#131209

Purpose

Cloning vector containing A-B-C casette with linker L2 in site B

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

GSSGGSSG linker

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_L1(GSSSG)

Plasmid

#131208

Purpose

Cloning vector containing A-B-C casette with linker L1 in site B

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

GSSSG linker

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAEF6

Plasmid

#136303

Purpose

pAEF5 plasmid with two gRNA inserted

Depositor

Gilles Fischer

Article

Agier et al Methods Mol Biol. 2021;2196:181-198. doi

Insert

double cassette coding for gRNA

Use

CRISPR; Template for pcr to insert double grna co…

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCLOCK-donor

Plasmid

#112310

Purpose

CRISPR donor plasmid to tag human transcription factor CLOCK with GFP

Depositor

Kevin White

Insert

CLOCK homology arms flanking EGFP-IRES-Neo cassette (CLOCK Human)

Use

CRISPR

Mutation

hg38:4:55435551:A>G, hg38:4:55435749:G>A

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRC02

Plasmid

#114681

Purpose

sfGFP.Strand10-Haloenzyme

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

sfGFP.Strand10-Haloenzyme

Tags

His10

Expression

Bacterial

Promoter

T7

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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KG#230

Plasmid

#110925

Purpose

Vector for expressing proteins in a subset of 9 DA/ DB cholingergic motor neurons in the C. elegans ventral nerve cord

Depositor

Kenneth Miller

Article

Edwards et al J Cell Biol. 2009 Sep 21;186(6):881-95.

Inserts

unc-129 promoter
unc-54 3' control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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BASIC_10_J23101-RBS32-GFP-B0015

Plasmid

#68144

Purpose

storage vector for BASIC part: GFP expression cassette

Depositor

Geoffrey Baldwin

Article

Storch et al ACS Synth Biol. 2015 Apr 16.

Insert

J23101-RBS32-GFP-B0015

Expression

Bacterial

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pFLIP30

Plasmid

#65499

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-mCer-cMyc

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cyEGFP-spo5DSR

Plasmid

#49867

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pFLIP32

Plasmid

#65500

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-AFPt9-attR1 and attR2-t7CFPt9-cMyc

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFLIP39

Plasmid

#65507

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMyc

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pMD18T-AtU6-HV5

Plasmid

#239470

Purpose

Encodes a gRNA expression cassette driven by the AtU6 promoter.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

U6-gRNA

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMD18T-AtU3b-HV5

Plasmid

#239477

Purpose

Encodes a gRNA expression cassette driven by the AtU3b promoter.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

U3b-gRNA

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMD18T-At7SL-HV5

Plasmid

#239478

Purpose

Encodes a gRNA expression cassette driven by the At7SL promoter.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

7SL-gRNA

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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EKAREN5-CFPonly

Plasmid

#246759

Purpose

Control plasmid for EKAREN5 without YPet cassette (expressing CFP only)

Depositor

Toru Hiratsuka

Article

Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

Insert

EKAREN5-CFPonly

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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EKAREN5-YFPonly

Plasmid

#246760

Purpose

Control plasmid for EKAREN5 without mTurquioise2 cassette (expressing YFP only)

Depositor

Toru Hiratsuka

Article

Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

Insert

EKAREN5-YFPonly

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET-hisPNK

Plasmid

#216670

Purpose

Expresses his-tagged PNK in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

T4 Polynucleotide Kinase (pseT T4 phage)

Tags

his-tag

Expression

Bacterial

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGMF6

Plasmid

#242208

Purpose

35Sp::eGFP:35St cassette in L1 vector backbone. Expresses a nuclear localized eGFP protein for transient plant transformation.

Depositor

Kate Creasey Krainer

Article

Abate et al bioRxiv 2025.11.09.687425

Insert

Level1 35Sp::eGFP:35St

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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