164,577 results

1. pHH0103_CAPNS1_EF-hand_8+EF-hand_5

   Plasmid

   #109995

   Purpose

   Protein expression and purification of CAPNS1_EF-hand_8+EF-hand_5

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   CAPNS1_EF-hand_8+EF-hand_5 (CAPNS1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRE-Tn7-160

   Plasmid

   #118572

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   TagBFP2
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSC-NGN2-IRES-GFP-T2A-Sox11

   Plasmid

   #90214

   Purpose

   To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with ISL1, LHX3, FGF2 and two small molecules, forskolin and dorsomorphin.

   Depositor

   Chun-Li Zhang

   Article

   Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

   Insert

   NGN2-IRES-GFP-T2A-Sox11 (NEUROG2, Sox11 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Sport6-CD63-pHuji

   Plasmid

   #130902

   Purpose

   Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

   Depositor

   DM Pegtel

   Article

   Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

   Insert

   CD63-pHuji (CD63 synthetic, Human)
   

   Use

   Tags

   pHuji

   Expression

   Mammalian

   Mutation

   pHuji inserted between Gln-36 and Leu-37 in extra…

   Promoter

   CMV

   Available Since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-THT

   Plasmid

   #55790

   Purpose

   GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Kanamycin resistance

   Depositor

   Stephan Geley

   Article

   Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

   Type

   Empty backbone

   Use

   RNAi; Gateway

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   modified H1 RNA gene

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF0346

   Plasmid

   #143397

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MYT1L (MYT1L Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-sgStk11#2/Cre

   Plasmid

   #173653

   Purpose

   Expresses a Stk11-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Stk11 (Stk11 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRE-Tn5-157

   Plasmid

   #118539

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mClover3
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-2xFLAG-2xSTREP-CCND2-IRES-mCherry

   Plasmid

   #172627

   Purpose

   Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D2 and mCherry in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND2 (CCND2 Human)
   

   Use

   Lentiviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PKCalpha.FLAG

    Plasmid

    #10805

    Depositor

    Alex Toker

    Article

    Hodges et al Adv Exp Med Biol. 2002 . 506(Pt A):237-

    Insert

    PKC alpha (PRKCA Bovine)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D332G-please see comments below

    Promoter

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. FKBP1B

    Plasmid

    #73241

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    FKBP1B (FKBP1B Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

12. LD-RA

    Plasmid

    #209863

    Purpose

    Dimerization dependent fluorescent protein RA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddRFP A
    

    Use

    Tags

    Hairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF0861

    Plasmid

    #141740

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR1H4 (NR1H4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCasSA

    Plasmid

    #98211

    Purpose

    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9

    Depositor

    Quanjiang Ji

    Article

    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Human Myc-XIAP

    Plasmid

    #217627

    Purpose

    Mammalian expression of Myc tagged XIAP

    Depositor

    Ethan Lee

    Article

    Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

    Insert

    XIAP (XIAP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLTS

    Plasmid

    #59386

    Purpose

    Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Type

    Empty backbone

    Use

    Genome engineering

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

    Plasmid

    #111925

    Purpose

    Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_110μ
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    E31D, F92W, E104D. D133E at CaM

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.DEST.YFP

    Plasmid

    #32683

    Purpose

    Lentiviral cDNA/shRNA gateway destination vectors

    Depositor

    Ming-Sound Tsao

    Article

    Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. p2R3a-Tq2CFP-OcsT

    Plasmid

    #71268

    Purpose

    Entry clone containing Turqoise2. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    Tq2CFP
    

    Use

    Gateway

    Tags

    4xGly linker and octaline synthase terminator

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. eDrFGFR1

    Plasmid

    #183994

    Purpose

    Optically controlled eDrFGFR1 expression in mammalian cells

    Depositor

    Dmitry Gerashchenko, Vladislav Verkhusha

    Article

    Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

    Insert

    eDrFGFR1 (FGFR1 Human)
    

    Use

    Tags

    Ig kappa secretory signal, mCherry, Deinococcus r…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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