We narrowed to 9,202 results for: sacs

1. p415 Gcs1 R54K AxxA

   Plasmid

   #112649

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Gcs1 R54K AxxA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   R54K & Alanine substitution of C-terminal δL …

   Promoter

   Met25

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p415 Gcs1 Δ3xF AxxA

   Plasmid

   #112650

   Purpose

   Expression S.cerevisiae

   Depositor

   Blanche Schwappach

   Article

   Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

   Insert

   Gcs1 Δ3xF AxxA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   R54K, W304A, W307A, W310A, W349A, F353A

   Promoter

   Met25

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSP569

   Plasmid

   #139499

   Purpose

   Repair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiae

   Depositor

   Serge Pelet

   Article

   Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

   Insert

   pSTL1 24xPP7sl STL1 100 - 600
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   pSTL1

   Available since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS416-PTEF1-MCP-5M-ECM33_intron

   Plasmid

   #127600

   Purpose

   low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter, 5M-ECM33 intron inserted into URA3

   Depositor

   David Bartel

   Article

   Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

   Insert

   ECM33 intron with MS2 hairpins replacing sequence near 5′ end of intron
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. S3_007_026_MV1

   Plasmid

   #134419

   Purpose

   pYTKMV1-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   NLS_FdeR-N_2xNLS
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   none

   Promoter

   pGMP1 and pTDH3

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. S3_008_026_MV1

   Plasmid

   #134420

   Purpose

   pYTKMV1-pRPL18B_ NLS_FdeR_N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   NLS_FdeR-N_2xNLS
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   none

   Promoter

   pGMP1 and pRPL18B

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. S3_009_026_MV1

   Plasmid

   #134421

   Purpose

   pYTKMV1-pREV1_ NLS_FdeR-N_2NLS-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   NLS_FdeR-N_2xNLS
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   none

   Promoter

   pGMP1 and pREV1

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. S3_007_026_MV2

   Plasmid

   #134424

   Purpose

   pYTKMV2-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   NLS_FdeR-N_2xNLS
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   none

   Promoter

   pGMP1 and pTDH3

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. S3_007_026_096

   Plasmid

   #134410

   Purpose

   pYTK096-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

   Depositor

   Mattheos Koffas

   Article

   Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

   Inserts

   mCherry
   NLS_FdeR-N_2xNLS
   

   Use

   Tags

   none

   Expression

   Yeast

   Mutation

   none

   Promoter

   pGPM1 and pTDH3

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. S3_008_026_096

    Plasmid

    #134411

    Purpose

    pYTK096-pRPL18B_ NLS_FdeR_N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    NLS_FdeR-N_2xNLS
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGPM1 and pRPL18B

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pst39-NST-clone14

    Plasmid

    #133421

    Purpose

    S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).

    Depositor

    Gerhard Wagner

    Insert

    NIP1/SUI1/TIF5 (NIP1, TIF5, SUI1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ORF for Nip1 encodes aa 4-156 (not complete ORF),…

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. S3_009_026_096

    Plasmid

    #134412

    Purpose

    pYTK096-pREV1_ NLS_FdeR-N_2NLS-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    NLS_FdeR-N_2xNLS
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGMP1 and pREV1

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. S3_001_026_096

    Plasmid

    #134402

    Purpose

    pYTK096-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    NLS_FdeR-N
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGPM1 and pTDH3

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. S3_004_026_096

    Plasmid

    #134407

    Purpose

    pYTK096-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    FdeR-N_2xNLS
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGPM1 and pTDH3

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. S3_002_026_MV1

    Plasmid

    #134414

    Purpose

    pYTKMV1-pRPL18B_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    NLS_FdeR-N
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGMP1 and pRPL18B

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. S3_006_026_096

    Plasmid

    #134409

    Purpose

    pYTK096-pREV1_FdeR-N_2NLS_tPGK1- pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    FdeR-N_2xNLS
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGPM1 and pREV1

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. S3_006_026_096

    Plasmid

    #134418

    Purpose

    pYTK096-pREV1_FdeR-N_2NLS_tPGK1- pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    FdeR-N_2xNLS
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGMP1 and pREV1

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. S3_004_026_MV2

    Plasmid

    #134423

    Purpose

    pYTKMV2-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    FdeR-N_2xNLS
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGMP1 and pTDH3

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. S3_003_026_096

    Plasmid

    #134406

    Purpose

    pYTK096-pREV1_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    NLS_FdeR-N
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGPM1 and pREV1

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. S3_005_026_MV1

    Plasmid

    #134417

    Purpose

    pYTKMV1-pRPL18B_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    FdeR-N_2xNLS
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGMP1 and pRPL18B

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. S3_003_026_MV1

    Plasmid

    #134415

    Purpose

    pYTKMV1-pREV1_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    NLS_FdeR-N
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGMP1 and pREV1

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. S3_001_026_MV1

    Plasmid

    #134413

    Purpose

    pYTKMV1-pTDH3_NLS_FdeR-N_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    NLS_FdeR-N
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGPM1 and pTDH3

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. S3_005_026_096

    Plasmid

    #134408

    Purpose

    pYTK096-pRPL18B_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

    Depositor

    Mattheos Koffas

    Article

    Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

    Inserts

    mCherry
    FdeR-N_2xNLS
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    none

    Promoter

    pGPM1 and pRPL18B

    Available since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. MTK234_050

    Plasmid

    #123918

    Purpose

    Encodes the saCas9 gRNA GFP dropout expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    SaCas9-sgRNA-GFPDROPOUT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGS-BacA-21222-FLP-3xFLAG

    Plasmid

    #109165

    Purpose

    Encodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Jensen et al Protein J. 2017 Aug;36(4):332-342. doi:

    Insert

    Flp
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRS-ATG1(mut)(406)

    Plasmid

    #122069

    Purpose

    Expressing a mutant ATG1

    Depositor

    Daniel Klionsky

    Article

    Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

    Insert

    ATG1 (ATG1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon changes without changing the amino acid seq…

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRS-ATG7p-ATG1(406)

    Plasmid

    #122071

    Purpose

    Expressing ATG1

    Depositor

    Daniel Klionsky

    Article

    Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

    Insert

    ATG1 (ATG1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The native promoter was replaced by ATG7 promoter…

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRS-Dhh1-PA(D195A,E196A)(405)

    Plasmid

    #122065

    Purpose

    Expressing Dhh1-PA

    Depositor

    Daniel Klionsky

    Article

    Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

    Insert

    Dhh1-PA (DHH1 Budding Yeast)
    

    Use

    Tags

    PA

    Expression

    Yeast

    Mutation

    Aspartic acid 195 to alanine; Glutamic acid 196 t…

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRS-Dhh1-PA(S226A,T228A)(405)

    Plasmid

    #122066

    Purpose

    Expressing Dhh1-PA

    Depositor

    Daniel Klionsky

    Article

    Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

    Insert

    Dhh1-PA (DHH1 Budding Yeast)
    

    Use

    Tags

    PA

    Expression

    Yeast

    Mutation

    Serine 226 to alanine; Threonine 228 to alanine

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGAD-Atg17(∆77-91)

    Plasmid

    #121516

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    deleted amino acids 77-91

    Promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge