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Showing: 10301 - 10350 of 167212 results

  1. pENTRY4_ Pi21

    Plasmid
    #126904
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126904-plasmid-map-sequence-id-249827

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  2. HC080

    Plasmid
    #59545
    Purpose
    Expresses yeast Atg13 under the endogenous promoter with a GFP N-terminal tag
    Depositor
    Daniel Klionsky
    Insert
    ATG13 (ATG13 Budding Yeast)
    Tags
    GFP
    Expression
    Bacterial and Yeast
    Mutation
    AvrII site created after start codon
    Promoter
    ATG13
    Available Since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pBS3ndd

    Plasmid
    #97354
    Purpose
    Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, kanamycin resistant
    Depositor
    Jamorn Somana
    Insert
    Nucleoid Disruptive Domain
    Use
    Cloning
    Expression
    Bacterial
    Mutation
    changed methionine 1 to isoleucine
    Promoter
    E. coli lacZ promoter
    Available Since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    97354-plasmid-map-sequence-id-191848

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  5. 5C8G

    Plasmid
    #69968
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Tb427tmp.01.1830:MAC054-B05:C234722
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. cTRE-GFP-miR30_shPten.1523

    Plasmid
    #135667
    Purpose
    Introduce Dox-inducible (TRE promoter) GFP cDNA plus miR30-embedded shPten into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    shPten
    Use
    Mouse Targeting
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135667-plasmid-map-sequence-id-325441

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  7. mEmerald-Talin-ABM-10

    Plasmid
    #54268
    Purpose
    Localization: Talin Actin Binding Domain, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Talin-ABM
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Aactin Binding Module; I/LWEQ = 197 amino acids fā€¦
    Promoter
    CMV
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pAc5.1B-lambdaN-HA-DmUPF3-V5His6_D

    Plasmid
    #146098
    Purpose
    Insect Expression of DmUPF3
    Depositor
    Elisa Izaurralde
    Insert
    DmUPF3 (Upf3 Fly)
    Expression
    Insect
    Mutation
    5 silent and non silent mutation P305S compared tā€¦
    Available Since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    146098-plasmid-map-sequence-id-362924

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  11. pGEX-5X-1 SmB-N

    Plasmid
    #129075
    Purpose
    generation of GST-tagged N-terminal part of SmB protein using bacteria
    Depositor
    Takeshi Tsubata
    Insert
    SmB (SNRPB Human)
    Expression
    Bacterial
    Mutation
    contains amino acids 1-492
    Available Since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    129075-plasmid-map-sequence-id-252121

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  12. pCMV6-XL4 FLAG-TM9SF4-Fc

    Plasmid
    #115770
    Purpose
    Expression of the extracellular domain of TM9SF4 with N-terminal FLAG tag and C-terminal hIgG tag.
    Depositor
    Davide Comoletti
    Insert
    TM9SF4 (TM9SF4 Human)
    Tags
    FLAG and hIgG/Fc
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    115770-plasmid-map-sequence-id-223833

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  13. R777-E205 Hs.PTEN

    Plasmid
    #70489
    Purpose
    Gateway ORF clone of human PTEN [NM_000314.5] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PTEN (PTEN Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70489-plasmid-map-sequence-id-125938

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  15. g6s pDONR

    Plasmid
    #117454
    Purpose
    Gateway Vector for Cyth3
    Depositor
    Richard Kahn
    Insert
    Cyth3 (CYTH3 Human)
    Use
    Gateway shuttling vector
    Available Since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    117454-plasmid-map-sequence-id-226897

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  17. NUDT14A

    Plasmid
    #42485
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT14A
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pRK GFP Vinculin

    Plasmid
    #50531
    Depositor
    Kenneth Yamada
    Insert
    Vinculin (VCL Chicken)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. U6-Lin28aIR

    Plasmid
    #120530
    Purpose
    dsRNA expression
    Depositor
    Petr Svoboda
    Insert
    Lin28 (Lin28a Mouse)
    Use
    RNAi
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120530-plasmid-map-sequence-id-233972

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  21. Nup54-mEGFP494(flex1)

    Plasmid
    #163430
    Purpose
    Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a flexible linker of 6 amino acids at the carboxyl end of the mEGFP
    Depositor
    Sanford Simon
    Insert
    Nup54 (NUP54 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163430-plasmid-map-sequence-id-321672

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  22. Nup54-mEGFP494(1)

    Plasmid
    #163427
    Purpose
    Mammalian expression of Nup54 with a mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of the mEGFP
    Depositor
    Sanford Simon
    Insert
    Nup54 (NUP54 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163427-plasmid-map-sequence-id-321664

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  23. R777-E252 Hs.RASSF1-nostop

    Plasmid
    #70536
    Purpose
    Gateway ORF clone of human RASSF1 [NM_007182.4] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASSF1 (RASSF1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70536-plasmid-map-sequence-id-125985

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  24. mCerulean-Vinculin-23

    Plasmid
    #55396
    Purpose
    Localization: Focal Adhesions, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    Vinculin (VCL Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  25. 4ROJ

    Plasmid
    #64661
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    VAV2:JMC092-C08:C231327 (VAV2 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    May 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. PP-UEV1A

    Plasmid
    #25111
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PFC0255c:C34858
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. BcatMut-Tyr604

    Plasmid
    #24196
    Depositor
    David Rimm
    Insert
    beta-catenin (CTNNB1 Human)
    Expression
    Mammalian
    Mutation
    Y604F
    Available Since
    March 9, 2010
    Availability
    Academic Institutions and Nonprofits only

  30. mEos2-SiT-N-15

    Plasmid
    #57418
    Purpose
    Localization: Golgi TGN, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    SiT (ST6GAL1 Human)
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    aa 1-45 of SiT
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. pORTMAGE302A

    Plasmid
    #128970
    Purpose
    Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with high temperature induction
    Depositor
    George Church
    Inserts
    PapRecT
    mutL E32K
    Expression
    Bacterial
    Mutation
    E32K
    Promoter
    cI-pL
    Available Since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    128970-plasmid-map-sequence-id-252505

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  32. PB CAG-R-3xSV40pA-R-td-sfGFP

    Plasmid
    #177150
    Purpose
    CAG tdGFP dre/rox reporter piggyBac transposon (3x SV40pA stop)
    Depositor
    Mario Capecchi
    Insert
    Tandem dimer superfolder GFP
    Use
    Dre / rox ; piggybac
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    177150-plasmid-map-sequence-id-348857

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  33. pBac[6xP3-Tc-hsp-DsRedExpress-SV40]

    Plasmid
    #86448
    Purpose
    pBAc transposon with transformation marker for Tribolium transgenesis; Drives dsRedExpress (red fluorescent) expression in the eyes (much stronger than usual 3XP3); (Transformation plasmid)
    Depositor
    Gregor Bucher
    Insert
    6xP3-Tc-hsp-DsRedExpress-SV40
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86448-plasmid-map-sequence-id-170875

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  34. 830 pcDNA3 GFP PTEN 1-336

    Plasmid
    #10927
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Amino acids 1-336
    Available Since
    Jan. 3, 2006
    Availability
    Academic Institutions and Nonprofits only

  35. pAW8-aid-3HA-eDSR-Turg1:kanMX6

    Plasmid
    #169363
    Purpose
    C-terminal aid-3HA-eDSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Tags
    3XHA
    Expression
    Yeast
    Available Since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169363-plasmid-map-sequence-id-332979

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  36. pAW8-aid-5Myc-eDSR-Turg1:kanMX6

    Plasmid
    #169364
    Purpose
    C-terminal aid-5Myc-eDSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Tags
    5XMyc
    Expression
    Yeast
    Available Since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169364-plasmid-map-sequence-id-332978

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  37. Abr in pAcG2T

    Plasmid
    #38162
    Depositor
    Nora Heisterkamp
    Insert
    ABR (ABR Human)
    Tags
    GST
    Expression
    Insect
    Promoter
    polyhedron
    Available Since
    Aug. 27, 2012
    Availability
    Academic Institutions and Nonprofits only

  38. pCMV6-XL4 FLAG-TMEFF2-Fc

    Plasmid
    #115774
    Purpose
    Expression of the extracellular domain of TMEFF2 with N-terminal FLAG tag and C-terminal hIgG tag.
    Depositor
    Davide Comoletti
    Insert
    TMEFF2 (TMEFF2 Human)
    Tags
    FLAG and hIgG/Fc
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    115774-plasmid-map-sequence-id-223834

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  39. R777-E312 Hs.SHC3-nostop

    Plasmid
    #70596
    Purpose
    Gateway ORF clone of codon optimized human SHC3 [NM_016848.5] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    SHC3 (SHC3 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70596-plasmid-map-sequence-id-126045

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  40. pTWEEN-miR-224

    Plasmid
    #35683
    Depositor
    George Calin
    Insert
    nsa-mir-224 (MIR224 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  43. R777-E290 Hs.RPS6KA1-nostop

    Plasmid
    #70574
    Purpose
    Gateway ORF clone of human RPS6KA1 [NM_002953.3] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RPS6KA1 (RPS6KA1 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70574-plasmid-map-sequence-id-126023

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  44. mTurquoise-Endo-14

    Plasmid
    #55549
    Purpose
    Localization: Endosomes, Excitation: 434, Emission: 474
    Depositor
    Michael Davidson
    Insert
    Endo (RHOB Human)
    Tags
    mTurquoise
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  48. pJW1328

    Plasmid
    #69488
    Purpose
    sgRNA(F+E) targeting pha-1 (no Cas9); for co-conversion in C. elegans
    Depositor
    Jordan Ward
    Insert
    sgRNA(F+E) targeting pha-1
    Expression
    Worm
    Promoter
    R07E5.16 U6 promoter
    Available Since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    69488-plasmid-map-sequence-id-125217

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Showing: 10301 - 10350 of 167212 results