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We narrowed to 14,223 results for: Cas9

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Showing: 4121 - 4140 of 14223 results

  3. B-HypaSpCas9

    Plasmid
    #126763
    Purpose
    Expression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-HypaSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N692A; M694A; Q695A; H698A; amino acids 1005-1013…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126763-plasmid-map-sequence-id-410968

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  5. CGBE1-NG (pBM1246)

    Plasmid
    #140256
    Purpose
    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eUNG-BE4max(R33A)_NG-ΔUGI
    Expression
    Mammalian
    Mutation
    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140256-plasmid-map-sequence-id-291778

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  7. dSaCas9-VPR

    Plasmid
    #205251
    Purpose
    In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495
    Depositor
    Rasmus Bak
    Publication
    Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d
    Insert
    dSaCas9-VPR
    Use
    CRISPR
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    205251-plasmid-map-sequence-id-408439

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  9. pCMV_eA3A-NG-nCas9

    Plasmid
    #163536
    Purpose
    Mammalian CG-to-GC base editing
    Depositor
    David Liu
    Publication
    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eA3A-NG-nCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…
    Available since
    Dec. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    163536-plasmid-map-sequence-id-320176

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  15. pLenti gRNA KO OCLNx2 spCas9-T2A-iRFP670-P2A-puro

    Plasmid
    #208399
    Purpose
    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, iRFP670 fluorescent protein and puromycin resistance
    Depositor
    Raphael Gaudin
    Publication
    Pradel et al Autophagy. 2024 Aug;20(8):1825-1836. doi
    Insert
    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    Use
    CRISPR and Lentiviral
    Tags
    iRFP670
    Expression
    Mammalian
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    208399-plasmid-map-sequence-id-417652

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  16. pLenti gRNA KO OCLNx2 spCas9-T2A-Crimson-P2A-puro

    Plasmid
    #208398
    Purpose
    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, E2-Crimson fluorescent protein and puromycin resistance
    Depositor
    Raphael Gaudin
    Publication
    Pradel et al Autophagy. 2024 Aug;20(8):1825-1836. doi
    Insert
    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    Use
    CRISPR and Lentiviral
    Tags
    E2-Crimson
    Expression
    Mammalian
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    208398-plasmid-map-sequence-id-417651

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  17. pLenti gRNA KO OCLNx2 spCas9-T2A-mNeonGreen-P2A-puro

    Plasmid
    #208400
    Purpose
    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, mNeonGreen fluorescent protein and puromycin resistance
    Depositor
    Raphael Gaudin
    Publication
    Pradel et al Autophagy. 2024 Aug;20(8):1825-1836. doi
    Insert
    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    Use
    CRISPR and Lentiviral
    Tags
    mNeonGreen
    Expression
    Mammalian
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    208400-plasmid-map-sequence-id-417650

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Showing: 4121 - 4140 of 14223 results