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L4440-daf-16b

Plasmid

#31504

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16b specific (daf-16 Nematode)

Use

RNAi

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
pSC300

Plasmid

#31820

Depositor

Yossef Av-Gay

Article

Cowley et al Gene. 2001 Feb 21;264(2):225-31.

Insert

GFP (lacking ATG start codon)

Tags

GFP (lacking ATG start codon)

Expression

Bacterial

Promoter

SOD

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pQE-WT-KIF-R5-IRH

Plasmid

#63629

Purpose

K1F-R5-IRH RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

KIF-R5-IRH RNAP

Mutation

L749I, Q754S, R756N, I761V, H772R, and Q786H

Promoter

T5/Lac

Available Since

June 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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L4440-daf-16af

Plasmid

#31506

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16a/f common (daf-16 Nematode)

Use

RNAi

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pET21b-RL018A

Plasmid

#42137

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mCerulean-mCherry

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

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pRSET B-BKAR

Plasmid

#14877

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2005 Feb 18. 280(7):5581-7.

Insert

B kinase activity reporter

Tags

CFP and YFP

Expression

Bacterial

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pME-NLS-tdMCP

Plasmid

#86245

Purpose

Middle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

NLStdMCP

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pBD-0082

Plasmid

#12369

Depositor

Axel Brunger

Article

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP K524A (Vcp Mouse)

Tags

His6

Expression

Bacterial

Mutation

K524A

Available Since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only
pGMV-gSWEET4

Plasmid

#112796

Purpose

Universal BeYDV-derived vector with gRNAs targeting the VviSWEET4 gene

Depositor

Humberto Prieto

Article

Prieto et al In: Cantu D., Walker M. (eds) The Grape

Insert

gRNA pair targeting VviSWEET4

Use

CRISPR

Expression

Plant

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBD-0072

Plasmid

#12361

Depositor

Axel Brunger

Article

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP E578Q (Vcp Mouse)

Tags

His6

Expression

Bacterial

Mutation

E578Q

Available Since

Sept. 8, 2006

Availability

Academic Institutions and Nonprofits only
pFastBac-Giardia Dicer

Plasmid

#89146

Purpose

Expression of Giardia Dicer in Sf9 cells

Depositor

Jennifer Doudna

Article

MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

Insert

Giardia Dicer

Tags

His6-teve

Expression

Insect

Available Since

April 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-MVK mazei

Plasmid

#108947

Purpose

heterologous expression of MVK of M. mazei in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Mevalonate kinase (MVK)

Tags

His6-Tag

Expression

Bacterial

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET21b-RL016A

Plasmid

#42135

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mYPet-mCherry

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

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pSuper.mBeta826

Plasmid

#12549

Depositor

Robert Sobol

Article

Trivedi et al Cancer Res. 2005 Jul 15. 65(14):6394-400

Insert

shRNA to mouse polymerase beta

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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T_antiEGFPsynsrRNA_UA

Plasmid

#185974

Purpose

Expresses antiEGFPsynsrRNA from PlTetO-1 promoter in E.coli Dh5alpha cell. For experiment use Dh5alphaz1.

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biosens Bioelectron. 2020 Nov 1;167:1124

Insert

PlTetO-1 AntiEGFPsynsrRNA

Use

Synthetic Biology

Promoter

PlTetO-1

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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L_EGFP_AC

Plasmid

#185971

Purpose

Expresses EGFP from PlLaco-1 promoter in E.coli Dh5alpha cell. For experiment use Dh5alphaz1

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biosens Bioelectron. 2020 Nov 1;167:1124

Insert

Pl-LacO1-Egfp

Use

Synthetic Biology

Promoter

PlLacO-1

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET21b-RL027A

Plasmid

#42133

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

mCherry-mVenus

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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Gsp_collagen

Plasmid

#107622

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

collagen

Use

RNAi; In situ hybridization

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEx_Hsp70::EGFPpA-ttBtagRFPt

Plasmid

#48877

Purpose

PhiC31 exchange vector with the Hsp70::EGFPpA insert. Selection via EGFP to tagRFPt-color switch in the heart

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al Development. 2013 Sep 18.

Insert

HSP70::EGFPpA

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pBD-0011

Plasmid

#12367

Depositor

Axel Brunger

Article

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP R599K (Vcp Mouse)

Tags

His6

Expression

Bacterial

Mutation

R599K

Available Since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only