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pGEL854

Plasmid

#214254

Purpose

Expression eTd-CBE in rice cells

Depositor

Yong Zhang

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Type

Empty backbone

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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B2401_miRFP-FKBP5x

Plasmid

#215118

Purpose

Expresses miRFP-FKBP-FKBP-FKBP-FKBP-FKBP in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

miRFP-FKBP-FKBP-FKBP-FKBP-FKBP

Tags

miRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pJTT002

Plasmid

#204493

Purpose

Reporter circuit

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

TagBFP
EYFP
mKate2

Use

Synthetic Biology

Expression

Bacterial and Mammalian

Promoter

hEF1a

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCfuNca-Mito

Plasmid

#190819

Purpose

Mitochondria targeted GFP for diatoms

Depositor

Peter Kroth

Article

Sprecher et al Algal Research. 2023 May; Vol 72: 103136

Inserts

Diatom mitochondrial targeted GFP
Bleomycin resistance gene

Expression

Bacterial

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-CtDCP2_1-267_X

Plasmid

#148004

Purpose

Bacterial Expression of CtDCP2_1-267

Depositor

Elisa Izaurralde

Article

Valkov et al Nat Struct Mol Biol. 2016 Jun;23(6):574-

Insert

CtDCP2_1-267

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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SL-TREAT

Plasmid

#196918

Purpose

The TREAT reporter containing cap-proximal GC-rich stemloop

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-ShadowY

Plasmid

#193555

Purpose

Expresses mEGFP-ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

ShadowY

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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SpAGS-dGoLoco1-GFP

Plasmid

#185577

Purpose

for overexpression

Depositor

Mamiko Yajima

Article

Poon et al Nat Commun. 2019 Aug 22;10(1):3779. doi:

Insert

AGS-dGoLoco1

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

deletion of GoLoco motif #1 (aa473 DNFFEALSRFQSNR…

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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Rp51a Hyperstabilized 5'SS

Plasmid

#100988

Purpose

pUC18 Hyperstabilized Rp51a 5'SS between HindIII and EcoRI; ATG/GTATGT -> AAG/GTAAGT

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

5'ss mut ATG/GTATGT -> AAG/GTAAGT

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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RP51A-NoBS-NoPseudoBS

Plasmid

#100986

Purpose

RP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BS

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Expression

Bacterial and Yeast

Mutation

BS mutated to GTTAGTG, pseudo BS mutated to GTTTG…

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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C121-E01: rLuc

Plasmid

#162888

Purpose

Standard Gateway entry clone encoding Renilla luciferase

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Renilla luciferase

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD4L1

Plasmid

#153154

Purpose

Expression of Flag tagged human FOXD4L1in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD4L1 (FOXD4L1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE96

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD4L3

Plasmid

#153155

Purpose

Expression of Flag tagged human FOXD4L3 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD4L3 (FOXD4L3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE97

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pSUI-SynP-CAS1sg

Plasmid

#154341

Purpose

Expression of the sgRNA targeting to the E. coli can gene

Depositor

I-Son Ng

Article

Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

Insert

CAS1 sgRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSUI-SynP-CAS2sg

Plasmid

#154342

Purpose

Expression of the sgRNA targeting to the E. coli can gene

Depositor

I-Son Ng

Article

Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

Insert

CAS2 sgRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSUI-SynP-CANTsg

Plasmid

#154343

Purpose

Expression of the sgRNA targeting to the E. coli can gene

Depositor

I-Son Ng

Article

Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

Insert

CANT sgRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSM-dCas9::Ap

Plasmid

#154307

Purpose

Shuttle vector for expression dCas9, mobilization cluster, expresses the tracrRNA and a CRISPR array designed for the easy cloning of new spacers.

Depositor

I-Son Ng

Article

Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSUI-SynP-sfGFP

Plasmid

#154306

Purpose

Expression of sfGFP under Synthetic promoter (SynP) and cloning of the sgRNA with simple screening of positive clone by fluorescence

Depositor

I-Son Ng

Article

Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pSUI-SynP-CAPsg

Plasmid

#154344

Purpose

Expression of the sgRNA targeting to the E. coli can gene

Depositor

I-Son Ng

Article

Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

Insert

CAP sgRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-BRAT

Plasmid

#117251

Purpose

mRNA synthesis

Depositor

Gerald Thomsen

Article

Horb et al Development. 1997 May;124(9):1689-98.

Insert

BRAT (vegt-a Frog)

Use

Xenopus expression

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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