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We narrowed to 16,181 results for: nes

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Showing: 4121 - 4140 of 16181 results

  2. pCMV-ABE8eWQ

    Plasmid
    #161815
    Purpose
    Expresses ABE8eWQ in mammalian cells
    Depositor
    Sangsu Bae
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    161815-plasmid-map-sequence-id-320654

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  7. pPMQAK1-Ptrc-[E*](104)-Cas9-B0015-lacZ-PnrsB-mazF-LVA

    Plasmid
    #190790
    Purpose
    [E*](104) construct. Theophylline inducible Cas9 (S. pyogenes) expression. Two BsaI-sites allow for simultaneous insertion of sgRNA and donor DNA. Includes a nickel-inducible curing system.
    Depositor
    Paul Hudson
    Publication
    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102
    Inserts
    Cas9
    mazF-LVA
    Use
    CRISPR and Synthetic Biology
    Tags
    LVA
    Expression
    Bacterial
    Mutation
    silent mutations of BpiI-sites in original Cas9 g…
    Promoter
    PnrsB and Ptrc-[E*](104)
    Available since
    Feb. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    190790-plasmid-map-sequence-id-384200

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  8. pSH-Csy4-T2A-SpRFN

    Plasmid
    #85754
    Purpose
    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).
    Depositor
    Lawrence Stanton
    Publication
    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy4-T2A-FokI-dCas9
    Use
    CRISPR
    Tags
    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    Jan. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    85754-plasmid-map-sequence-id-170272

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  16. CaTCH Dual-sgRNA_sgBC2-A_sgBC2-C

    Plasmid
    #154197
    Purpose
    Lentiviral expression plasmid encoding two sgRNAs without a target. These can be used as a off-target control for CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.
    Depositor
    Anna Obenauf
    Publication
    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103
    Insert
    sgRNA-BC2-A, sgRNA-BC2-C
    Use
    Lentiviral; Catch barcode activation
    Expression
    Mammalian
    Promoter
    hU6 and mU6
    Available since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154197-plasmid-map-sequence-id-308682

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  17. NRCoC

    Plasmid
    #74300
    Purpose
    Mammalian expression of full-length treacle fused to GFP
    Depositor
    Rita Shiang
    Publication
    Winokur et al Hum Mol Genet. 1998 Nov;7(12):1947-52.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    treacle ribosome biogenesis factor 1 (Tcof1 Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    74300-plasmid-map-sequence-id-173857

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  20. pAAV-UbC-eGFP-F

    Plasmid
    #71545
    Purpose
    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.
    Depositor
    Pantelis Tsoulfas
    Insert
    eGFP-F
    Use
    AAV
    Tags
    contains 20- amino-acid farnesylation signal from…
    Expression
    Mammalian
    Promoter
    UbC
    Available since
    Dec. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    71545-plasmid-map-sequence-id-132622

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Showing: 4121 - 4140 of 16181 results