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AiP12025 - pAAV-3xSP10ins-AiE0367h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2025)

Plasmid

#191716

Purpose

AI plasmid ALIAS: AiP12025. Expression of SYFP2 (yellow fluorescent protein) in striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Mutation

none

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12514 - pAAV-3xSP10ins-AiE0367h_C2-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2514)

Plasmid

#191717

Purpose

AI plasmid ALIAS: AiP12514. Expression of SYFP2 (yellow fluorescent protein) in striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Mutation

none

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMAL-PUM1

Plasmid

#120384

Purpose

Bacterial expression of MBP-Pumillio1 fusion protein

Depositor

Igor Ulitsky

Publication

Tichon et al Nat Commun. 2016 Jul 13;7:12209. doi: 10

Insert

PUM1 (PUM1 Human)

Tags

6xHIS and MBP

Expression

Bacterial

Promoter

LacI

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.2/NS2A

Plasmid

#120818

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Publication

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NS2A ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-Src

Plasmid

#118308

Purpose

Expression of mouse Src

Depositor

Philip Stork

Publication

Schmitt et al Mol Cell. 2002 Jan;9(1):85-94.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Src (Src Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-IKZF2-V2-V5

Plasmid

#107391

Purpose

Mammalian expression of IKZF2-V2-V5

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF2-V2 (IKZF2 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-Rac1 G15A

Plasmid

#69355

Purpose

expression of nucleotide free Rac1 in bacterial cells

Depositor

Rafael Garcia-Mata

Publication

Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

Insert

Rac1 (RAC1 Human)

Tags

GST

Expression

Bacterial

Mutation

G15A

Promoter

tac

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only
Mis12-targted Aurora B FRET sensor

Plasmid

#45231

Depositor

Michael Lampson

Publication

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mis12 targeted Aurora B FRET sensor

Expression

Mammalian

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-p53

Plasmid

#39479

Depositor

Ie-Ming Shih

Publication

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

TP53 (TP53 Human)

Tags

GST

Expression

Bacterial

Mutation

P72R

Available since

Sept. 25, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
DAXX-myc

Plasmid

#1851

Depositor

Philip Leder

Publication

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

DAXX (Daxx Mouse)

Tags

myc

Expression

Bacterial

Available since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
pS148∙CsR

Plasmid

#197858

Purpose

Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria

Depositor

Susanne Häußler

Publication

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

gRNA scaffold - Ap/Cb resistance cassette - incP oriT

Use

CRISPR and Synthetic Biology

Promoter

PEM7

Available since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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CBh-dCas9-Mll3SET

Plasmid

#108452

Purpose

Expression vector of CRISPR/dCas9 fused with SET domain of mouse MLL3 gene, driven by CBh promoter

Depositor

Bing Ren

Publication

Yan et al Cell Res. 2018 Jan 9. pii: cr20181. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9, MLL3 (Kmt2c Mouse)

Expression

Mammalian

Promoter

CBh

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-Halo-Tev-Keap1

Plasmid

#62456

Purpose

His6-halo tagged human Keap1 protein for E.coli expression

Depositor

Yimon Aye

Publication

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Bacterial

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)

Plasmid

#78509

Purpose

Construct for expressing CD45R0 (extracellular domain) His10 in Sf9

Depositor

Ron Vale

Publication

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

CD45R0Ex (PTPRC Human)

Tags

His10 and SNAPf

Expression

Insect

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hAIM2-T7

Plasmid

#51538

Purpose

Express T7-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Publication

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AIM2 (AIM2 Human)

Tags

T7

Expression

Mammalian

Promoter

CMV

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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BDS-2 3x MUT (p53 binding site)

Plasmid

#16516

Depositor

Bert Vogelstein

Publication

Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

Insert

p53 binding site (SFN Human)

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant p53 binding sites

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pTRE-BI-hGRAD

Plasmid

#207837

Purpose

Inducible hGRAD Plasmids for rapid depletion of GFP-tagged proteins in mammalian cells

Depositor

Michaela Mueller-McNicoll

Publication

Arnold et al J Cell Biol. 2024 Feb 5;223(2):e20230403

Inserts

mCherry
hFBXW11 (FBXW11 Human)
VHH-GFP4

Expression

Mammalian

Mutation

215 amino acids of the full length protein

Promoter

TRE3G BI promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJKR-H-acuR

Plasmid

#62567

Purpose

Acrylate biosensor. High copy plasmid that expresses GFP proportional to acrylate concentration.

Depositor

George Church

Publication

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLX307-VHL-3F-BirA

Plasmid

#220147

Purpose

Expresses VHL-BirA for VHL substrate identification by E-STUB

Depositor

William Sellers

Publication

Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

Insert

von Hippel-Lindau tumor suppressor (VHL Human)

Use

Lentiviral

Tags

3F-BirA

Expression

Mammalian

Mutation

codon optimized

Promoter

EF-1a

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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plenti-px330-CRBN-T1-pGK-Pur

Plasmid

#107382

Purpose

Mammalian expression CRISPR/Cas9

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

sgRNA targeting CRBN (CRBN Human)

Use

Lentiviral; Crispr

Expression

Mammalian

Promoter

U6

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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