We narrowed to 6,654 results for: SIM;

1. pLV-SI-112

   Plasmid

   #131127

   Purpose

   C-to-T base editing reporter

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Mutant EGFP
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. MTK0_051

   Plasmid

   #123948

   Purpose

   Encodes the hAAVS1 homology landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   hAAVS1 landing pad destination vector
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCA15-Ubc-NLS- scFV-tdTomato

   Plasmid

   #199446

   Purpose

   Expression of scFV-tdTomato that binds to the GCN4 peptide from the SunTag System in mammalian cells

   Depositor

   Baohui Chen

   Article

   Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

   Insert

   scFV-tdTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human ubiquitin C (UBC)

   Available since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2

   Plasmid

   #169531

   Purpose

   Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGFP-PTEN

   Plasmid

   #50519

   Purpose

   This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Article

   Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

   Insert

   PTEN (PTEN Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pdUA-lacIN

   Plasmid

   #141079

   Purpose

   Plasmid vector for testing DNA binding proteins by the I-Block assay.

   Depositor

   Antal Kiss

   Article

   Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28

   Type

   Empty backbone

   Use

   Vector for the i-block assay designed to study dn…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Escherichia coli lacIN promoter

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMDJ21 - pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]

   Plasmid

   #159810

   Purpose

   Optimized enzyme for genome editing in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FDIO-dAPEX2

   Plasmid

   #117175

   Purpose

   Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   dAPEX2
   

   Use

   AAV; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-FLJ90652

   Plasmid

   #15359

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

   Insert

   FLJ90652 (INO80E Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQTEV-GYG

    Plasmid

    #31324

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    glycogenin (GYG1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-GAS41

    Plasmid

    #15428

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    GAS41 (Yeats4 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pSI-359

    Plasmid

    #131131

    Purpose

    gRNA expression vector for A-to-G base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    A-to-G reporter activation gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOPINK-TRABID (NZF1, aa 1-33)

    Plasmid

    #65437

    Purpose

    Expresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coli

    Depositor

    David Komander

    Article

    Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

    Insert

    TRABID (ZRANB1 Human)
    

    Use

    Tags

    His6-GST

    Expression

    Bacterial

    Mutation

    only codes for amino acids 1-33 of TRABID, which …

    Promoter

    T7

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

    Plasmid

    #119074

    Purpose

    Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    ubb:MCP-p65-HSF1-2a-Cerulean
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish ubiquitin promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVC-Ds-ubb:Com-KRAB-2a-Cerulean-Ds

    Plasmid

    #119075

    Purpose

    Zebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporter

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    ubb:Com-KRAB-2a-Cerulean
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish ubiquitin promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVC-Ds-DrU6a:sgRNA-com-Ds

    Plasmid

    #119077

    Purpose

    To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loops

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:2xCom-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVC-Ds-DrU6a:sgRNA-MS2-Ds

    Plasmid

    #119076

    Purpose

    To clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loops

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    U6a:2xMS2-sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Zebrafish U6a promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TB:7xSMAD_RE:MiniP

    Plasmid

    #133879

    Purpose

    7 copies the SMAD DNA binding motif

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    7 copies the SMAD DNA binding motif
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMZ827

    Plasmid

    #155321

    Purpose

    Constitutive expression of E-PIF6(1-100) in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-E-PIF6(1-100)-NLS-Tnos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pROF051

    Plasmid

    #155328

    Purpose

    Constitutive expression of SRDX-EL222 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-SRDX-NLS-EL222-Tnos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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