162,586 results

1. pAAV-CAG-(n1)QC2-6 FIQ(c4)-P2A-FusionRed

   Plasmid

   #229573

   Purpose

   Expresses (n1)QC2-6 FIQ(c4) and FusionRed in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

   Insert

   (n1)QC2-6 FIQ(c4)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-SOD2 MTS-3xHA-TALE array-Nt.BspD6I(C)-P2A-EGFP-UTR

   Plasmid

   #235204

   Purpose

   Plasmids for constructing mitoBEs v2

   Depositor

   Wensheng Wei

   Article

   Zhang et al Nature. 2025 Jan 22. doi: 10.1038/s41586

   Insert

   SOD2 MTS-3xHA-TALE array-Nt.BspD6I(C)-P2A-EGFP-UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-COX8 MTS-3xFlag-TALE array-TadA8e(V106W, V28F)-P2A-mCherry-UTR

   Plasmid

   #235203

   Purpose

   Plasmids for constructing mitoBEs v2

   Depositor

   Wensheng Wei

   Article

   Zhang et al Nature. 2025 Jan 22. doi: 10.1038/s41586

   Insert

   COX8 MTS-3xFlag-TALE array-TadA8e(V106W, V28F)-P2A-mCherry-UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. NanoLuc-PTPN2 Fusion Vector

   Plasmid

   #237182

   Purpose

   Express NanoLuc(R)-PTPN2 Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   PTPN2 (PTPN2 Human)
   

   Use

   Tags

   NanoLuc (R)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. SmBiT-ADCY8 BiBiT-Ready Vector

   Plasmid

   #237115

   Purpose

   Express SmBiT-ADCY8 Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   ADCY8 (ADCY8 Human)
   

   Use

   Tags

   SmBiT

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. LgBiT-CALM1/SmBiT-ADCY1.1 BiBiT Vector

   Plasmid

   #237118

   Purpose

   Express LgBiT-CALM1/SmBiT-ADCY1.1 Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Inserts

   CALM1 (CALM1 Human)
   ADCY1 (ADCY1 Human)
   

   Use

   Tags

   LgBiT and SmBiT

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pNL [NlucP/AP1-RE/Hygro] Vector

   Plasmid

   #236811

   Purpose

   Express AP1 response element in luciferase reporter assay

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   AP1
   

   Use

   Luciferase

   Tags

   NanoLuc (R)

   Expression

   Mutation

   None

   Promoter

   minP

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pcDNA3.1-HaloAKAR2.1-T2A-EGFP

   Plasmid

   #216489

   Purpose

   Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAKAR2.1
   

   Use

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTYB3-T4gp43(WT)

   Plasmid

   #228203

   Purpose

   Expression in BL21(DE3) of WT T4 DNA polymerase

   Depositor

   Stephen Benkovic

   Insert

   43
   

   Use

   Tags

   Sce VMA intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pStreptagIIx2 Alfatag 3C InsR EABR

    Plasmid

    #234996

    Purpose

    For production of Extracellular Vesicles (EVs), with the Insulin Receptor Strep-tag II labeled on their surface

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    InsR-EABR (INSR Human)
    

    Use

    Tags

    2xStreptag-II, Alfatag, HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pStreptagII 3C SMO

    Plasmid

    #234995

    Purpose

    Expression of a Strep-tag II labeled on its N-terminus receptor, with the Smoothened (Smo) receptor

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    SMO (SMO Human)
    

    Use

    Tags

    Streptag-II, HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHA TST 3C SMO EABR

    Plasmid

    #234989

    Purpose

    For production of Extracellular Vesicles (EVs), with the receptor Smoothened (Smo), Twin-Strep-tag (TST), and an HA tag at its N-terminus on their surface

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    SMO-EABR (SMO Human)
    

    Use

    Tags

    HA, Twin-Strep-Tag, HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEABR NA 3C FLAG SA

    Plasmid

    #234987

    Purpose

    For production of Extracellular Vesicles (EVs), with the transmembrane region of Neuraminidase protein fused to Streptavidin on the vesicles surface

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    EABR-NA
    

    Use

    Tags

    HRV 3C site, FLAG, Strep-Tactin (SA)

    Expression

    Mammalian

    Mutation

    In the Streptavidin coding sequence Glu44Val/Ser4…

    Promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. hNTa2-qgRNA-pYJA5

    Plasmid

    #217780

    Purpose

    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning

    Depositor

    Adriano Aguzzi

    Article

    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

    Insert

    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6, mouse U6, human H1, human 7SK

    Available since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pStreptagII 3C EGFR EABR

    Plasmid

    #234994

    Purpose

    For production of Extracellular Vesicles (EVs), with Epithelial Growth Factor Receptor on their surface, Strep-tag II labeled on its N-terminus

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    EGFR-EABR (EGFR Human)
    

    Use

    Tags

    Streptag-II, HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBS CMV NA 3C FLAG SA

    Plasmid

    #234993

    Purpose

    Expression of transmembrane region of Neuraminidase protein fused to streptavidin protein for displaying on viral like particles (VLPs) when cotranfected with GAG protein

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    NA
    

    Use

    Tags

    HRV 3C site, FLAG, Strep-Tactin (SA)

    Expression

    Mammalian

    Mutation

    In the Streptavidin coding sequence Glu44Val/Ser4…

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNA 3C myc scfvFLAG

    Plasmid

    #234990

    Purpose

    Expression of transmembrane region of Neuraminidase protein fused to an anti FLAG scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG protein

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    NA-3C-myc-scfvFLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHA StreptagII 3C NIS EABR

    Plasmid

    #234988

    Purpose

    For production of Extracellular Vesicles (EVs), with a Strep-tag II labeled Sodium-iodide symporter (NIS), and an HA epitope at its N-terminus on their surface

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    NIS-EABR (SLC5A5 Human)
    

    Use

    Tags

    HA, Streptag-II, HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNA 3C myc scfvHA

    Plasmid

    #234991

    Purpose

    Expression of transmembrane region of Neuraminidase protein fused to an anti HA scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteins

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    NA-3C-myc-scfvHA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. psLIB CMV

    Plasmid

    #229953

    Purpose

    bigMamAct is evolution of biGBac for mammalian cells. Has empty CMV-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

    Depositor

    Martin Pelosse

    Article

    Pelosse et al Synthetic Biology 2025.02.13

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge