We narrowed to 70,757 results for: LAS

1. pMC_mClover3_BSDminus_F0

   Plasmid

   #184165

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mClover3
   bsd
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRR-ERalpha-5Z

   Plasmid

   #23061

   Depositor

   Charles Miller

   Article

   Miller et al Plasmid. 2009 Dec 3. ():.

   Insert

   ERalpha (ESR1 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pSW002-PpsbA_mTurquoise2

   Plasmid

   #108237

   Purpose

   Broad host bacterial expression vector with constitutive PsbA promoter. Expresses mTurquoise2; codon optimized for P. fluorescens.

   Depositor

   Rosemarie Wilton

   Article

   Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

   Insert

   PsbA promoter - mTurquoise2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT85_SpdCas9_pmcDA1

   Plasmid

   #204614

   Purpose

   CRISPR cytosine deaminase integrative plasmid for Mycoplasma bovis

   Depositor

   Pascal Sirand-Pugnet

   Article

   Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMC_mClover3_BSDminus_F2

   Plasmid

   #184167

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mClover3
   bsd
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMC_mClover3_BSDminus_F1

   Plasmid

   #184166

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mClover3
   bsd
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-Myo 1g

   Plasmid

   #134991

   Purpose

   Expression of mouse Myo 1g in mammalian cells.

   Depositor

   Martin Bähler

   Article

   Olety et al FEBS Lett. 2010 Feb 5;584(3):493-9. doi:

   Insert

   Myo 1g (Myo1g Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. YSEHPTFTSQY_epitope

   Plasmid

   #165016

   Purpose

   Expression of CMV UL83 (363-373) fused to IL-2 signal sequence

   Depositor

   Matthew Meyerson

   Article

   Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

   Insert

   CMV UL83 (363-373)
   

   Use

   Lentiviral

   Tags

   human IL2 signal sequence at N-term

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-EGFP-C1-HsNot11_K

   Plasmid

   #146776

   Purpose

   Mammalian Expression of HsNot11

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

   Insert

   HsNot11 (CNOT11 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAtCHYb1Δ129N

    Plasmid

    #53367

    Purpose

    Contains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

    Insert

    chyB1 (BETA-OHASE 1 Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deleted codons for the first 129 amino acids

    Promoter

    Trc

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti HsATP10B_WT-T2A-His-flag-TMEM30A variant1

    Plasmid

    #191675

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP10B WT and His/Flag tagged Hs TMEM30A

    Depositor

    Veerle Baekelandt

    Inserts

    ATP10B (ATP10B Human)
    TMEM30A (TMEM30A Human)
    

    Use

    Lentiviral

    Tags

    6X his and flag

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CRISPR_TRAC

    Plasmid

    #164993

    Purpose

    Expression of gRNA targeting TCRalpha constant locus

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against TRAC
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLG1-puro Non-targeting sgRNA 1

    Plasmid

    #109002

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJYDN3p

    Plasmid

    #162457

    Purpose

    Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.

    Depositor

    Gideon Schreiber

    Article

    Zahradník et al bioRxiv Preprint 2020

    Insert

    Aga2p-eUnaG2 (AGA2 )
    

    Use

    Shuttle vector bacteria/yeast

    Tags

    ALFA tag, HA and myc tags, and eUnaG2 fluorescent…

    Expression

    Mutation

    Promoter

    GAL1

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-Tal1

    Plasmid

    #60670

    Purpose

    PiggyBac expression vector encoding murine Tal1 downstream of a doxycycline inducible promoter

    Depositor

    James Thomson

    Article

    Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

    Insert

    Tal1 (Tal1 Mouse)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Pprm1-luciferase; his-3

    Plasmid

    #137728

    Purpose

    Communication-activated luciferase in Neurospora crassa

    Depositor

    Louise Glass

    Article

    Fischer et al Genetics. 2019 Apr;211(4):1255-1267. doi

    Inserts

    Luciferase
    Pprm-1 Promoter
    

    Use

    Luciferase; Neurospora crassa expression

    Tags

    Expression

    Mutation

    Codon-optimized for N. crassa

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCngA2-ISH

    Plasmid

    #15855

    Depositor

    Peter Mombaerts

    Article

    Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

    Insert

    Cyclic Nucleotide Gated Channel Alpha 2 (Cnga2 Mouse)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pSW002-PpsbA

    Plasmid

    #108236

    Purpose

    Broad host bacterial expression vector with constitutive PsbA promoter

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    PsbA promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Blat-BSD

    Plasmid

    #163791

    Purpose

    The plasmid expresses human codon-optimized BlatCas9, gRNA expression elements and blasticidin resistence gene.

    Depositor

    Yongming Wang

    Article

    Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Geph CR3m shRNA

    Plasmid

    #121069

    Purpose

    Negative control shRNA for plasmid 121068: shRNA targeting the coding region of the gephyrin mRNA

    Depositor

    Angel de Blas

    Article

    Yu et al Mol Cell Neurosci. 2007 Dec;36(4):484-50

    Insert

    GPHN shRNA (Gphn Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. CRISPR_TRBC

    Plasmid

    #164994

    Purpose

    Expression of gRNA targeting TCRbeta constant locus

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against TRBC
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pARNTL.1.0-gDNA

    Plasmid

    #112480

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ARNTL

    Depositor

    Kevin White

    Insert

    ARNTL (BMAL1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. VYAIFIFQL_epitope

    Plasmid

    #165018

    Purpose

    Expression of CMV US20 (29-37) fused to IL-2 signal sequence

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    CMV US20 (29-37)
    

    Use

    Lentiviral

    Tags

    human IL2 signal sequence at N-term

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAM/CBA-eGFP-miRaSyn(mouse)x3-WPRE-bGHpA

    Plasmid

    #194248

    Purpose

    Expresses 3 miRNAs targeting mouse alpha-Synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    miRaSynuclein(mouse) (Snca Mouse)
    

    Use

    AAV and RNAi

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET28a-Ec.coaBC (pCLK1210)

    Plasmid

    #50448

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

    Depositor

    Tadhg Begley, Erick Strauss

    Article

    Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

    Insert

    PPCS PPCDC
    

    Use

    Tags

    6XHIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAT15483_e-ABE8e

    Plasmid

    #174121

    Purpose

    Expresses ABE8e fused with eCas9

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    eABE8e
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. PB-Pitx2

    Plasmid

    #60668

    Purpose

    PiggyBac expression vector encoding murine Pitx2 downstream of a doxycycline inducible promoter

    Depositor

    James Thomson

    Article

    Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

    Insert

    Pitx2 (Pitx2 Mouse)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAT15001-AncBE4max

    Plasmid

    #163016

    Purpose

    plasmid expressing AncBE4max

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    AncBE4max
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AAV-SYN-PIK3CD

    Plasmid

    #203730

    Purpose

    AAV vector plasmid expressing human phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) under the human synapsin (SYN) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    PIK3CD (PIK3CD Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human synapsin promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHEY1.1.0-gDNA

    Plasmid

    #112393

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor HEY1

    Depositor

    Kevin White

    Insert

    HEY1 (HEY1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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