We narrowed to 39,988 results for: ANT

1. peGFP C3-SPG20-PAAA

   Plasmid

   #218581

   Purpose

   Expresses GFP-tagged PPAY motif mutant (PAAA) of human SPG20 in mammalian cells; this mutant does not interact with ITCH

   Depositor

   Hemmo Meyer

   Article

   Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

   Insert

   SPG20 (SPART Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   changed Proline 172 to Alanine and Tyrosine 174 t…

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-PDGFRB-V5/HIS

   Plasmid

   #201988

   Purpose

   expression of human PDGFRB receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   PDGFRB receptor tyrosine kinase (PDGFRB Human)
   

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPalmitoyl-mTurquoise2

   Plasmid

   #36209

   Purpose

   In vivo visualization of the membrane (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   p63(1-29) (ARHGEF25 Human)
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKrox24(MapErk)Luc

   Plasmid

   #200112

   Purpose

   Luciferase reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   MapErk sequence in minimal promoter
   

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   5 copies of designed MapErk sequence in minimal p…

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-TRKC-V5/HIS

   Plasmid

   #205615

   Purpose

   Expression of human TRKC receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   TRKC receptor tyrosine kinase (NTRK3 Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1/CALR wt-FLAG

   Plasmid

   #204532

   Purpose

   Mammalian expression of human CALR wt-FLAG

   Depositor

   Norio Komatsu

   Article

   Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

   Insert

   CALR wt-FLAG (CALR Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-UbC

   Plasmid

   #62806

   Purpose

   To express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.

   Depositor

   Pantelis Tsoulfas, Jae Lee

   Article

   Soderblom et al Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/

   Type

   Empty backbone

   Use

   AAV; Eucaryotic

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMP71-tCD19-mGFP

   Plasmid

   #174609

   Purpose

   expresses membrane-associated (palmitoylated) EGFP and extracellular and transmembrane domains of murine CD19

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   CD19 extracellular and transmembrane domain (Cd19 Mouse)
   

   Tags

   palmitoylated EGFP

   Expression

   Mammalian

   Available Since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUSE-CHIg-hG1_AT118-L F47T, Y98F-Fc LALA + PG

   Plasmid

   #222854

   Purpose

   Mammalian expression plasmid for secretion of a non-AT1R binding control AT118-L variant fused to human IgG1 Fc

   Depositor

   Andrew Kruse

   Article

   Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

   Insert

   AT118-L
   

   Tags

   IgG1_Fc

   Expression

   Mammalian

   Mutation

   L234A, L235A, P329G

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCCLc-MND-A0201-SABR-Backbone

    Plasmid

    #119050

    Purpose

    3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MND

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKrox24(MapErk)dTomato

    Plasmid

    #200113

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    MapErk sequence in minimal promoter
    

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    5 copies of designed MapErk sequence, no other pr…

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT1-24X-NOG(AtUBI10)

    Plasmid

    #202016

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Inserts

    dCas9-24XGP41
    NbGP41P-sfGFP-VP64-GB1
    

    Tags

    GB1 and sfGFP

    Expression

    Plant

    Promoter

    AtUBI10

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKrox24(2xD-E)dTomato

    Plasmid

    #200110

    Purpose

    Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    modified EGR1 promoter
    

    Expression

    Mammalian

    Mutation

    highly modified sequence

    Promoter

    2 copies of D-E element, no other promoter elemen…

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-RET-V5/HIS

    Plasmid

    #205606

    Purpose

    Expression of human RET receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    RET receptor tyrosine kinase (RET Human)
    

    Tags

    V5-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. eNS2B47NS3 L30SF31S

    Plasmid

    #155319

    Purpose

    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker L30SF31S mutant

    Depositor

    Dahai Luo

    Article

    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

    Insert

    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    L30 to S, F31 to S

    Promoter

    M13

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. nCas9-PPE

    Plasmid

    #140445

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

    Insert

    nCas9(H840A)-M-MLV
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    H840A for Cas9; D200N, T306K, W313F, T330P and L…

    Promoter

    maize Ubiquitin-1

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5-FLAG-SLC38A9.1

    Plasmid

    #71855

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNA 3C myc scfvHA

    Plasmid

    #234991

    Purpose

    Expression of transmembrane region of Neuraminidase protein fused to an anti HA scFv antibody fragment for displaying on Viral like particles (VLPs) when cotranfected with GAG proteins

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    NA-3C-myc-scfvHA
    

    Expression

    Mammalian

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCEP4-myc-HLA-A24

    Plasmid

    #135507

    Purpose

    Mammalian expression of myc-tagged HLA-A*024:02

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*24:02 (HLA-A Human)
    

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-BCR-ABL-p190-FLAG

    Plasmid

    #205620

    Purpose

    Expression of BCR-ABL oncogene in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

    Insert

    BCR-ABL oncogene, p190 isoform b3a2
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T117M- Please see depositor comments

    Promoter

    CMV

    Available Since

    Sept. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLJM60-FLAG-SLC38A9.1

    Plasmid

    #71858

    Purpose

    lentiviral stable expression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSG5-HA-p300-DY1399

    Plasmid

    #89095

    Purpose

    mammalian expression of full-length human p300 with DY1399 mutation, acetylation mutant, acetyltransferase activity defect

    Depositor

    Elizabeth Wilson

    Article

    Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

    Insert

    p300-D1399Y mutant (EP300 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    p300-DY1399

    Promoter

    SV40

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Cav3-YFP

    Plasmid

    #69507

    Purpose

    Expression of fluorescently tagged Caveolin-3 in mammalian cells

    Depositor

    Rob Parton

    Article

    Pol et al Mol Biol Cell. 2004 Jan;15(1):99-110. Ep

    Insert

    Cav3 (Cav3 Mouse)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pWZ699

    Plasmid

    #208117

    Purpose

    Payload yeast assembly vector with TK-mActb on backbone

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    Synthetic Biology; Copy number inducible

    Expression

    Bacterial, Mammalian, and Y…

    Promoter

    Human PGK1 for thymidine kinase

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. EGFR G719S

    Plasmid

    #11013

    Purpose

    Retroviral construct for expressing human EGFR with G719S mutation in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR G719S (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    G719S

    Available Since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

26. TACR1-DuET

    Plasmid

    #213385

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    TACR1 (TACR1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1/MPL-V5

    Plasmid

    #204531

    Purpose

    Mammalian expression of human MPL-V5

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    MPL-V5 (MPL Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MRGPRX1-DuET

    Plasmid

    #213344

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MRGPRX1 (MRGPRX1 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pWZ403

    Plasmid

    #208113

    Purpose

    mSwAP-In marker cassette 1 with mScarlet-PuroTK

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Insert

    mScarlet-PuroTK
    

    Tags

    P2A

    Expression

    Bacterial and Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRN3P-HA-Suv4-20h2mut

    Plasmid

    #86692

    Purpose

    For transcription of of Suv4-20h2mut mRNA preceded by an HA-tag in 5'

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

    Insert

    histone-lysine N-methyltransferase KMT5C mutant (Kmt5c Mouse)
    

    Tags

    HA

    Mutation

    Mutated asparagine 273 to cysteine 276 (NHDC) int…

    Promoter

    T3

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCKC014

    Plasmid

    #226623

    Purpose

    pLenti wt hTdT template for library 3

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Insert

    Terminal deoxynucleotidyl transferase (DNTT Human)
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDx_mScarlet3-NCwt-SYFP2

    Plasmid

    #189766

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mScarlet3-NCwt-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet3-NCwt-SYFP2
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    encodes the expected internal mutations relative …

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDNR-SpCas9-Cdt1

    Plasmid

    #80426

    Purpose

    Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

    Depositor

    Sara Howden, Melissa Little

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Insert

    SpCas9-Cdt1
    

    Use

    CRISPR

    Tags

    Cdt1

    Mutation

    codon optimized for human

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. SiriusGFP

    Plasmid

    #123201

    Purpose

    We have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.

    Depositor

    Joseph Santos-Sacchi

    Article

    Zhong et al J Neurosci Methods. 2019 Feb 1;313:68-76

    Insert

    SiriusGFP
    

    Use

    Mouse Targeting

    Tags

    N/A

    Expression

    Bacterial and Mammalian

    Mutation

    eGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAGM22082_cRed

    Plasmid

    #117225

    Purpose

    pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

    Insert

    Canthaxanthin Operon
    

    Tags

    34 amino acid N-terminal linker, Hexahistidine Ta…

    Expression

    Bacterial

    Mutation

    removed Type IIS restriction sites within the bac…

    Promoter

    T7 RNA Polymerase

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pWZ854

    Plasmid

    #208116

    Purpose

    mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-Neo

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Insert

    mNeonGreen-HPRT1-Neo
    

    Tags

    T2A

    Expression

    Bacterial and Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pWZ852

    Plasmid

    #208114

    Purpose

    mSwAP-In marker cassette 1 with mScarlet-HygTK

    Depositor

    Jef Boeke

    Article

    Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

    Insert

    mScarlet-HygTK
    

    Tags

    T2A

    Expression

    Bacterial and Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCDNA3-HA-Akt1-K179M

    Plasmid

    #73409

    Purpose

    Expresses kinase-inactive mutant of Akt with HA tag

    Depositor

    Jie Chen

    Article

    Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

    Insert

    AKT1 (AKT1 Human)
    

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    changed K179M to make Kinase inactive mutant

    Promoter

    CMV

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLpA-DN.JNK2

    Plasmid

    #51943

    Purpose

    Adenoviral vector for dominant negative JNK2 expression

    Depositor

    Jeffery Molkentin

    Article

    Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

    Insert

    DN JNK2 (Mapk9 Mouse)
    

    Use

    Adenoviral

    Tags

    HA

    Mutation

    dominant negative: the dual phosphorylation moti…

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pcDNA3.1-RON-V5/HIS

    Plasmid

    #201983

    Purpose

    expression of human RON receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    RON receptor tyrosine kinase (MST1R Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge