We narrowed to 18,467 results for: Met

1. pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9

   Plasmid

   #84261

   Purpose

   Expresses AND gate ABA/GA-inducible VPR-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sp dCas9
   PYL1-VPR-p2a-GID1-ABI
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, GAI, GID1, HA tag, PYL1, p2a, …

   Expression

   Mammalian

   Promoter

   CAG and PGK

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVUHshp53-tTR-KRAB

   Plasmid

   #11654

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for shp53 (hUbiquitin promoter) - 3rd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hUbiquitin C, GFP, tTR-KRAB, shRNA against TP53, no tetO (TP53 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CBh-IgKL-mNG

   Plasmid

   #183467

   Purpose

   To produce AAV with the CBh promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

   Depositor

   Hajime Hirase

   Article

   Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

   Insert

   IgKL-mNeonGreen
   

   Use

   AAV

   Tags

   IgKL secretory signal

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmScarlet-H_Giantin_C1

   Plasmid

   #85049

   Purpose

   In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   Giantin (GOLGB1 Human)
   

   Expression

   Mammalian

   Mutation

   aa 3131-3259

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpA

   Plasmid

   #89874

   Purpose

   expresses DRD2-iTango component in mammalian cells for AAV production

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   DRD2-V2tail-TevN-BLITz1-TetR-VP16
   

   Use

   AAV

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   short isoform of DRD2 (deletion of 242-270)

   Promoter

   Human synapsin

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-eGFP-attP(bxb)-*BsdR

   Plasmid

   #183751

   Purpose

   Bxb1 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   loxP-eGFP-attP-Bsd-lox251
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet-1_VNp-mNeongreen-StefinA

   Plasmid

   #182419

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-mNeongreen-stefin (CSTA Human)
   

   Tags

   Vesicle Nucleating peptide (VNp) & mNeongreen

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXS-IRES-Blast CHKA

   Plasmid

   #218540

   Purpose

   human CHKA cDNA

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   CHKA (CHKA Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBR_attB(bxb)_ccdB_lox

   Plasmid

   #183763

   Purpose

   Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   ccdB
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB1163

    Plasmid

    #210032

    Purpose

    Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas11-P2A-Cas6-T2A-Cas3
    rtta-T2A-blast
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMis1_1B

    Plasmid

    #192079

    Purpose

    A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction

    Depositor

    Markus Buchhaupt

    Article

    Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.

    Inserts

    KanR
    rhaR
    rhaS
    pBBR1 rep
    

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry

    Plasmid

    #35512

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. Pinducer20-BSD-scFv-sfGFP-DNMT3A1

    Plasmid

    #115780

    Purpose

    targeted DNA methylation tool

    Depositor

    Margaret Goodell

    Article

    Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

    Insert

    scFv-sfGFP-DNMT3A1 (DNMT3A Human)
    

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

    Plasmid

    #140018

    Purpose

    Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    EcoTyrRS
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y37L, D182S, F183M, L186A

    Promoter

    EF1

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSF-MjCouRS

    Plasmid

    #229066

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of (7-hydroxy-4-coumarin-yl) ethylglycine (Hco), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

    Insert

    Methanococcus jannaschii tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFP

    Plasmid

    #55642

    Purpose

    sCre-Dependent ChR2-EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    hChR2(H134R)-EYFP
    

    Use

    AAV; Scre-dependent

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Aug. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pECE M2-SH3PXD2A WT

    Plasmid

    #69813

    Purpose

    Expresses M2-SH3PXD2A in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SV40 early promoter

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. 20XUAS IVS ChrimsonR mCherry_tr

    Plasmid

    #111546

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of ChrimsonR mCherry

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR_mCherry_trafficked
    

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-sgp53/Cre

    Plasmid

    #89646

    Purpose

    Expresses a p53-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting p53 (Trp53 Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Bxb1_AJMA

    Plasmid

    #183760

    Purpose

    Bxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Inserts

    ASAP2f
    jRCaMP1b
    miRFP703
    ASAP2f
    

    Tags

    Lck and NES

    Expression

    Mammalian

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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