We narrowed to 562 results for: OAT

1. pAAV-U6-racRNA-D

   Plasmid

   #200827

   Purpose

   AAV transfer plasmid encoding a circularized RNA barcode D under U6 promoter and a subcellular localization protein binder under hSyn promoter

   Depositor

   Xiao Wang

   Article

   Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

   Inserts

   U6-racRNA
   V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far
   

   Use

   AAV

   Tags

   C-Far, Flag, and V5

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGLUE-SHLD2

   Plasmid

   #114118

   Purpose

   Expresses N-terminally tagged Streptag-HA-CBP-SHLD2 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD2 (SHLD2 Human)
   

   Use

   Tags

   HA tag, Streptavidin-binding tag (Streptag), and …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pDEST-FRT/TO-eGFP-REV7

   Plasmid

   #114128

   Purpose

   Expresses N-terminally tagged eGFP-REV7 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   REV7 (MAD2L2 Human)
   

   Use

   Genomic integration, tetracycline inducible

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available since

   Aug. 15, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pDEST-FRT/TO-3xFLAG-REV7

   Plasmid

   #114127

   Purpose

   Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   REV7 (MAD2L2 Human)
   

   Use

   Genomic integration, tetracycline inducible

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available since

   Aug. 16, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCW-eGFP-FHA-SHLD2-m1

   Plasmid

   #114122

   Purpose

   Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2, m1 mutant; 53BP1-independent recruitment to damaged chromatin

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD2 (m1 mutant) (SHLD2 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   RNF8 FHA domain (aa 2-160) and eGFP

   Expression

   Mammalian

   Mutation

   W489A, F494A, W495A mutations introduced

   Promoter

   TRE promoter, Tet ON

   Available since

   Aug. 28, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pHIV-NAT-FLAG-CIP2A ∆NES (del561-625)

   Plasmid

   #222625

   Purpose

   Lentiviral vector that expresses Flag-tagged CIP2A NES mutant in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

   Insert

   CIP2A (CIP2A Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deletion of the residues 561-625 (ΔNES)

   Promoter

   EF-1-alpha promoter

   Available since

   July 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pET283xFlagPP7CPHis

   Plasmid

   #28174

   Depositor

   Kathleen Collins

   Insert

   PP7 coat protein optimized
   

   Use

   Tags

   6xhistidine and triple FLAG peptide

   Expression

   Bacterial

   Mutation

   C68A,C73A

   Promoter

   Available since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. P22_wtCP_SP141his

   Plasmid

   #122651

   Purpose

   Expression of P22 virus like particle in bacteria

   Depositor

   Trevor Douglas

   Insert

   P22 Coat: WT / SP: 6xHis-SP141
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   truncated SP, P120H in the capsid scaffold (see …

   Promoter

   Available since

   Aug. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PHB1

   Plasmid

   #212153

   Purpose

   For PHB production in E. coli (PT7-pMB1).

   Depositor

   Kang Zhou

   Insert

   poly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PT7

   Available since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCSDest-ENTR-MCP-ADAR2-DD(E488Q/T490A)-V5

    Plasmid

    #192714

    Purpose

    Mammalian expression vector containing MS2 coat protein fused to ADAR2 catalytic domain (E488Q/T490A) with V5 tag

    Depositor

    Summer Thyme

    Insert

    MCP-ADAR2-DD(E488Q/T490A)-V5
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    E488Q/T490A

    Promoter

    CMV

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTwist-ENTR-MCP-ADAR2-DD(E488Q/T490A)-V5

    Plasmid

    #192713

    Purpose

    Gateway entry vector containing MS2 coat protein fused to ADAR2 catalytic domain (E488Q/T490A) with V5 tag

    Depositor

    Summer Thyme

    Insert

    MCP-ADAR2-DD(E488Q/T490A)-V5
    

    Use

    Gateway entry vector

    Tags

    V5

    Expression

    Mutation

    E488Q/T490A

    Promoter

    None

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTU1-D-spsCD

    Plasmid

    #112788

    Purpose

    Downstream homology arm, SpsC is involved in spore coat polysaccharide synthesis

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    spsCD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTU1-A-spsCU

    Plasmid

    #112787

    Purpose

    Upstream homology arm, SpsC is involved in spore coat polysaccharide synthesis

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    spsCU
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET22HT-PP7delFG

    Plasmid

    #31863

    Depositor

    Robert Singer

    Article

    Chao et al Nat Struct Mol Biol. 2008 Jan;15(1):103-

    Insert

    PP7delFG Coat Protein
    

    Use

    Tags

    His6-tag and TEV site

    Expression

    Mutation

    deleted FG loop

    Promoter

    Available since

    Aug. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. phage - ubc - nls - 2xmcp - mCherry - BirA*

    Plasmid

    #131136

    Purpose

    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

    Depositor

    Ralf Jansen

    Insert

    NLS-HA-2xMCP-mCherry-BirA*
    

    Use

    Lentiviral

    Tags

    NLS, HA

    Expression

    Mammalian

    Mutation

    BirA* = BIrA >R118G

    Promoter

    human ubiquitin C (UBC)

    Available since

    Aug. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMS2-1

    Plasmid

    #220627

    Purpose

    Plasmid used to place the RNA sequence of interest next to two MS2 coat protein recognition sites.

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. phage - ubc - nls - mcp - egfp - BirA*

    Plasmid

    #131134

    Purpose

    BirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

    Depositor

    Ralf Jansen

    Insert

    NLS-HA-MCP-EGFP-BirA*
    

    Use

    Lentiviral

    Tags

    NLS, HA

    Expression

    Mammalian

    Mutation

    BirA* = BIrA >R118G

    Promoter

    human ubiquitin C (UBC)

    Available since

    Aug. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMS2-2

    Plasmid

    #220628

    Purpose

    Plasmid allows you to place the RNA sequence of interest next to two MS2 coat protein recognition sites.

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. phage - ubc - nls - 2xmcp - egfp - cMycBirA*

    Plasmid

    #131133

    Purpose

    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

    Depositor

    Ralf Jansen

    Insert

    NLS-HA-2xMCP-EGFP-cMycBirA*
    

    Use

    Lentiviral

    Tags

    NLS, HA, cMyc (N-terminal of BirA*)

    Expression

    Mammalian

    Mutation

    BirA* = BIrA >R118G

    Promoter

    human ubiquitin C (UBC)

    Available since

    Aug. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTEF1_MS2

    Plasmid

    #220625

    Purpose

    Source for TEF1 MS2 coat fusion protein fusion

    Depositor

    Marvin Wickens

    Article

    Preston et al Nat Methods. 2019 May;16(5):437-445. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge