2,162 results

1. Tol2-LyzC-GFP-U6ac-rac2-guides

   Plasmid

   #168241

   Purpose

   "neutrophil specific GFP with ubiquitous rac2 sgRNAs"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   rac2 sgRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2z-tal1

   Plasmid

   #164656

   Purpose

   for the synthesis of zebrafish tal1 mRNA

   Depositor

   Didier Stainier

   Article

   Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

   Insert

   tal1 (tal1 Zebrafish)
   

   Use

   Mrna synthesis

   Tags

   Expression

   Mutation

   Promoter

   SP6

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDest-1.7kbfabp6-eGFP-pA-CrymCherry

   Plasmid

   #159088

   Purpose

   LR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA inserts

   Depositor

   John Rawls

   Article

   Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

   Inserts

   1.7kb fabp6 promoter (fabp6 Zebrafish)
   eGFP
   polyA
   

   Use

   Fluorescent reporter

   Tags

   Expression

   Mutation

   Promoter

   1.7kb fabp6

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. ZP36 Pax2

   Plasmid

   #16906

   Depositor

   Randall Moon

   Insert

   pax2 (pax2a Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pBS-I-Sce1-slc6a3[11kb]:egfp

   Plasmid

   #53240

   Purpose

   allow visualization of pretectal dopaminergic neurons in vivo from day 3 post-fertilization and allow expression of transgenes in these neurons through adulthood

   Depositor

   Edward Burton

   Article

   Bai et al Neuroscience. 2009 Dec 15;164(3):1138-51

   Insert

   DAT-GFP
   

   Use

   Cloning

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2-cryR;tmem88aCE1-P1Egfp

   Plasmid

   #90148

   Purpose

   contains tmem88a enhancer sequence, and tmem88a specific gene promoter driving expression of eGFP

   Depositor

   Nathan Lawson

   Article

   Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

   Insert

   tmem88aE1P1Egfp (tmem88a Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

   Plasmid

   #79887

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMT-myl7-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80060

   Purpose

   Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   Myl7 promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)

   Plasmid

   #140893

   Purpose

   Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cel

   Depositor

   Yasushi Okamura

   Article

   Kawanabe et al J Gen Physiol. 2020 May 4;152(5). pii: 1

   Insert

   CiDr-VSP L223F/C302S-mCherry (vsp, tpte Ciona intestinalis, Zebrafish)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   changed 223L to F in Dr-VSP (zebrafish TPTE), cha…

   Promoter

   Available Since

   May 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SI16-Kit a

    Plasmid

    #58948

    Purpose

    expresses recombinant mouse anti-Kit a receptor (zebrafish) IgG1 antibody in mammalian cells

    Depositor

    Gavin Wright

    Article

    Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

    Insert

    Kit receptor a mouse monoclonal antibody (kita Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pPRISM-Stop-cmlc2-mRFP

    Plasmid

    #117775

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    3x3 Stop sequence
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEM-T ZfHoxc11a

    Plasmid

    #27542

    Depositor

    Victoria Prince

    Insert

    Hoxc11a (hoxc11a Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pAC8-TwinStrep-3C-DrHDAC6 (40-831)

    Plasmid

    #180226

    Purpose

    Baculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)

    Depositor

    Patrick Matthias

    Article

    Langousis et al Methods Mol Biol. 2023;2589:467-480. doi

    Insert

    Histone deacetylase 6 (hdac6 Zebrafish)
    

    Use

    Tags

    TwinStrep-3C

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEM-T Easy-Gbait-hs-QF2

    Plasmid

    #122563

    Purpose

    Integration of QF2 sequence using the CRISPR/Cas9 system.

    Depositor

    Marnie Halpern

    Article

    Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

    Insert

    Gbait-hsp70-QF2-SV40pA
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. ZP16 Wnt-1

    Plasmid

    #16925

    Depositor

    Randall Moon

    Insert

    wnt-1 (wnt1 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pT2KXIGdeltaIn-MCS-birA-EGFP

    Plasmid

    #58377

    Purpose

    tol2 transgenesis for biotin based TRAP - BirA ligase

    Depositor

    Didier Stainier

    Article

    Housley et al Development. 2014 Oct;141(20):3988-93. d

    Insert

    birA EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2 you

    Plasmid

    #11084

    Depositor

    William Talbot

    Article

    Woods et al PLoS Biol. 2005 Mar . 3(3):e66.

    Insert

    you (scube2 Zebrafish)
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 8, 2005

    Availability

    Academic Institutions and Nonprofits only

18. ZE15 pCS2P+ wnt8 ORF2

    Plasmid

    #17049

    Depositor

    Randall Moon

    Insert

    wnt8 (wnt8a Zebrafish)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

19. p5E-6xgata2aECE

    Plasmid

    #132969

    Purpose

    gata2a endothelial enhancer (x6) in p5E Gateway vector

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    gata2a endothelial enhancer (x6)
    

    Use

    Gateway vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPRISM-Fuse-gcry-mRFP

    Plasmid

    #117784

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    mRFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge