2,246 results
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Plasmid#180012PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; HE1A: BFP
UseSynthetic BiologyAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV_PP1-mCitrine
Plasmid#172472PurposeMammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.DepositorInsertprolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
UseLentiviralTagsmCitrine and prolactin signal sequence (cleaved p…ExpressionMammalianPromoterEF1alphaAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDestTol2-actinb-kcna1a-IRES-EGFP
Plasmid#164965PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFPDepositorInsertkcna1a (kcna1a Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPPromoteractinb (actb2) promoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-EGFP
Plasmid#164940PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminusDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsEGFPMutationStop codon deletedPromoteractinb (actb2) promoterAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3E-EEA1
Plasmid#109560PurposeMultisite gateway vector for 5' tagging with early endosome associated 1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertearly endosome associated 1 (eea1 Zebrafish)
UseZebrafish plasmidsAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Rac1-DN
Plasmid#109530PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative Rac1DepositorAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-Rac1
Plasmid#109529PurposeMuscle specific zebrafish expression of mKate2 fused to Rac1DepositorAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-DysF
Plasmid#109492PurposeMuscle specific zebrafish expression of mKate2 fused to dysferlinDepositorAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-LA-nanoLuc-RA
Plasmid#128692PurposeDonor plasmid that promotes integration of the NanoLuc coding sequence at the apobb.1 locusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertapobb.1 NanoLuc
UseMultisite-gateway assemblyTagsNanoLucAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Cre-cmlc2-TagBFP
Plasmid#117792PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCre
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Cre-cmlc2-eGFP
Plasmid#117794PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCre
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-gcry-eGFP
Plasmid#117785PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmRFP
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-cmlc2-TagBFP
Plasmid#117786PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmRFP
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Stop-gcry-eGFP
Plasmid#117773PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3x3 Stop sequence
UseSynthetic BiologyAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEM-T Easy-Gbait-hs-Cre
Plasmid#122562PurposeIntegration of Cre sequence using the CRISPR/Cas9 system.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGbait-hsp70-Cre-SV40pA
UseCloningAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Halo-Tev- HSPB7 (zebrafish) WT
Plasmid#108916PurposeExpress WT Zebrafish Halo-Tev-HSPB7 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHalo-TEV-HSPB7 (hspb7 Zebrafish)
TagsHalo-TEVExpressionMammalianPromoterSP6Available sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-RFP-miR-223
Plasmid#97148PurposemiR-223 expressionDepositorInsertmiR-223
UseZebrafishAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
hsp70:mKate2-NWASP-CA
Plasmid#105967Purposeheat shock inducible transgenesisDepositorInsertNWASP-CA
TagsmKate2ExpressionBacterialAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;basPEgfp
Plasmid#90169Purposecontains a basal promoter driving expression of eGFPDepositorInsertbasalPEgfp
TagsEGFPExpressionBacterialAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4P1Egfp
Plasmid#90160Purposecontains dll4 specific gene promoter driving expression of eGFPDepositorAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only