We narrowed to 16,742 results for: EMB

1. PCUP1-GFP-Vma13-Ura

   Plasmid

   #207026

   Purpose

   Expression of GFP-Vma13 under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   Vma13 ORF
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. P1K-3HA-Atg8∆GR-MET15

   Plasmid

   #207033

   Purpose

   Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Use

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   ∆GR

   Promoter

   pATG8

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PCUP1-mRuby3-Pho8-406

   Plasmid

   #207043

   Purpose

   Expression of mRuby3-Pho8 under CPU1 promoter.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   PHO8
   

   Use

   Tags

   mRuby3

   Expression

   Yeast

   Mutation

   Promoter

   pCUP1

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NBla_1.2_GpA_G83I

   Plasmid

   #207844

   Purpose

   BLaTM-System GpA G83I negative control in NBLa 1.2 vector

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   N-BLa 1.2 fusion protein
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYea5.1_C99_sfGFP

   Plasmid

   #207802

   Purpose

   Yeast 2µ vector for expression of Suc2SP-C99-sfGFP-GAL4

   Depositor

   Dieter Langosch

   Article

   Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

   Insert

   Suc2SP-C100-sfGFP-GAL4 fusion protein
   

   Use

   Tags

   Strep-TagII

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYea5.1_Notch3_sfGFP

   Plasmid

   #207864

   Purpose

   Yeast 2µ vector for expression of Suc2SP-Notch3_100-sfGFP-GAL4

   Depositor

   Dieter Langosch

   Article

   Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

   Insert

   Suc2SP-Notch3_100-sfGFP-GAL4 fusion protein
   

   Use

   Tags

   Strep-TagII

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NBla_1.2_GpA_wt

   Plasmid

   #207842

   Purpose

   BLaTM-System GpA wt positive control in NBLa 1.2 vector

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   N-BLa 1.2 fusion protein
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CBLa_1.2_GpA_wt

   Plasmid

   #207843

   Purpose

   BLaTM-System GpA wt positive control in CBLa 1.2 vector

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   N-BLa 1.2 fusion protein
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CBLa_1.2_GpA_G83I

   Plasmid

   #207845

   Purpose

   BLaTM-System GpA G83I negative control in CBLa 1.2 vector

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   N-BLa 1.2 fusion protein
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. InterTag-1xL

    Plasmid

    #186911

    Purpose

    Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a single GGGGS linker and the other side expressing BFP and puromycin resistance gene

    Depositor

    Taekjip Ha, Allen Liu

    Article

    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

    Insert

    SpyTag003-sfCherry11-(GGGGS)1-PDGFR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRetroX-TRE3G_NINJ1

    Plasmid

    #201181

    Purpose

    Dox-inducible expression of NINJ1 gene in mammalian cells

    Depositor

    Jonathan Kagan

    Article

    Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

    Insert

    Ninjurin 1 (Ninj1 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-NINJ1

    Plasmid

    #201180

    Purpose

    Transient expression of NINJ1 gene in mammalian cells

    Depositor

    Jonathan Kagan

    Article

    Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

    Insert

    Ninjurin 1 (Ninj1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRN3P-NLS-EGFP-Btgh-3NLS

    Plasmid

    #194469

    Purpose

    Encodes for bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

    Depositor

    Mathieu Boulard

    Article

    Formichetti et al Dev Cell 10 Jan 2024

    Insert

    BtGH84
    

    Use

    For in vitro transcription from t3 promoter after…

    Tags

    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTAL-MajSat-FLAG-NLS-VP64

    Plasmid

    #69074

    Purpose

    Expresses TALE for mouse major satellite with VP64 and FLAG under CAG promoter

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

    Insert

    TALE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTAL-MajSat-FLAG-NLS-MCS

    Plasmid

    #69073

    Purpose

    Expresses TALE for mouse major satellite with FLAG tag under CAG promoter

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

    Insert

    TALE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. C1-F-tractin-mCherry

    Plasmid

    #155218

    Purpose

    Monitor global F-actin distribution

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mCherry (Itpka Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PADH1-TIR1-9myc-URA3

    Plasmid

    #207016

    Purpose

    Expresses TIR1-9Myc. TIR1 mediates auxin-induced ubiquitination of auxin-inducible degron (AID).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    TIR1
    

    Use

    Tags

    9xMyc

    Expression

    Yeast

    Mutation

    Promoter

    pADH1

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSCV-NINJ1

    Plasmid

    #201179

    Purpose

    Expression of NINJ1 gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

    Insert

    Ninjurin 1 (Ninj1 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. K44A dynamin 1 pEGFP

    Plasmid

    #34681

    Depositor

    Sandra Schmid

    Article

    Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

    Insert

    dynamin 1ba (DNM1L Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-3xGFP11-mem-3xControlgRNA

    Plasmid

    #224583

    Purpose

    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    3xGFP11
    

    Use

    CRISPR

    Tags

    Membrane Localization Signal

    Expression

    Mutation

    Codon optimized

    Promoter

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8

    Plasmid

    #207031

    Purpose

    Tet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    Pho8
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. C1-F-tractin-mCitrine

    Plasmid

    #155217

    Purpose

    Monitor global F-actin distribution

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TC1316

    Plasmid

    #164883

    Purpose

    CMV-bpNLS-dCas13d-bpNLS-ADARdd-mCherry, ADARdd embeded in loop3

    Depositor

    Tian Chi

    Article

    Liu et al EMBO J. 2020 Nov 16;39(22):e104748. doi:

    Insert

    dcas13d-ADARdd
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas13d L560-G586 deleted

    Promoter

    CMV, T7

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGFP_mEGFP-GluA1c

    Plasmid

    #114136

    Purpose

    Mammalian expression of the cytosolic C terminus of GluA1 N-terminally tagged by mEGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    mEGFP tagged GluA1 C-terminus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET17b-yebST

    Plasmid

    #175804

    Purpose

    Complementation of yebST knockout in E. coli K-12

    Depositor

    Ian Henderson, Gira Bhabha, Damian Ekiert

    Article

    Isom et al Sci Rep. 2017 Aug 17;7(1):8608. doi: 10.

    Insert

    yebST
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    silent mutations to introduce restriction sites

    Promoter

    T7

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. wt dynamin 1 pEGFP

    Plasmid

    #34680

    Depositor

    Sandra Schmid

    Article

    Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

    Insert

    dynamin 1ba (DNM1L Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

27. YFP Gamma 8

    Plasmid

    #36106

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 8 (GNG8 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

28. MSCV-mNINJ1-K45Q-GFP

    Plasmid

    #208777

    Purpose

    MSCV vector expressing K45Q mouse NINJ1

    Depositor

    Petr Broz

    Article

    Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

    Insert

    NINJ1 (Ninj1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Change Lys 45 to Gln (K45Q)

    Promoter

    Available since

    Aug. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. YFP Gamma 2

    Plasmid

    #36102

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein subunit Gamma 2 (GNG2 Bovine)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

30. pRRL CAG NFlag BAF170 ires GFP

    Plasmid

    #24562

    Depositor

    Jerry Crabtree

    Article

    Ho et al Proc Natl Acad Sci U S A. 2009 Mar 31. 1

    Insert

    BAF170 (Smarcc2 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2010

    Availability

    Academic Institutions and Nonprofits only

31. mFGF4

    Plasmid

    #22085

    Depositor

    Gail Martin

    Article

    Hebert et al Dev Biol. 1990 Apr . 138(2):454-63.

    Insert

    FGF4 (Fgf4 Mouse)
    

    Use

    Bacterial

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

32. mcherry Beta 1

    Plasmid

    #36111

    Depositor

    Narasimhan Gautam

    Article

    Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

    Insert

    G protein beta subunit B1 (GNB1 Bovine)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2012

    Availability

    Academic Institutions and Nonprofits only

33. MSCV-mNINJ1-D53A-GFP

    Plasmid

    #208778

    Purpose

    MSCV vector expressing D53A mouse NINJ1

    Depositor

    Petr Broz

    Article

    Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

    Insert

    NINJ1 (Ninj1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mutation

    Changed aspartic acid 53 to alanine (D53A)

    Promoter

    Available since

    Aug. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJAF215

    Plasmid

    #52402

    Purpose

    Expresses Flag-tagged mouse Arf1

    Depositor

    Gregory Pazour

    Article

    Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

    Insert

    Arf1 (Arf1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

35. C1-MPAct(delta1-6)-mRuby3

    Plasmid

    #155223

    Purpose

    F-actin bidning mutant of MPAct

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Mutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Deletion of first 6 AA from F-tractin (9-14 from …

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRRL CAGpNFlag BAF155 ires GFP

    Plasmid

    #24561

    Depositor

    Jerry Crabtree

    Article

    Ho et al Proc Natl Acad Sci U S A. 2009 Mar 31. 1

    Insert

    BAF155 (Smarcc1 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    July 16, 2010

    Availability

    Academic Institutions and Nonprofits only

37. C1-FT-mCitrine-EAAARx8-CaaX

    Plasmid

    #155224

    Purpose

    MPAct with increased search radius

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBTK615

    Plasmid

    #110609

    Purpose

    BTK assembled plasmid - used in Stage 2 assembly to target Cas9 or dCas9

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    sgRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHisAlix

    Plasmid

    #42576

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    ALIX (PDCD6IP Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Bacterial

    Mutation

    silent mutation A1617G (numbering in ALIX gene)

    Promoter

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

40. pJAF211

    Plasmid

    #52407

    Purpose

    Expresses Flag-tagged mouse Arf6

    Depositor

    Gregory Pazour

    Article

    Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

    Insert

    Arf6 (Arf6 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only